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Literature summary extracted from

  • Huy, N.D.; Thiyagarajan, S.; Kim, D.H.; Park, S.M.
    Cloning and characterization of a novel bifunctional acetyl xylan esterase with carbohydrate binding module from Phanerochaete chrysosporium (2013), J. Biosci. Bioeng., 115, 507-513.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.72 gene axe2, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Pichia pastoris Phanerodontia chrysosporium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.72 CO2 50% inhibition at 1 mM Phanerodontia chrysosporium
3.1.1.72 Cu2+ 50% inhibition at 1 mM Phanerodontia chrysosporium
3.1.1.72 Fe2+ strong, over 98% inhibition at 1 mM Phanerodontia chrysosporium
3.1.1.72 Mn2+ 50% inhibition at 1 mM Phanerodontia chrysosporium
3.1.1.72 additional information weak inhibition by PMSF, Tween20 and Tween80 Phanerodontia chrysosporium
3.1.1.72 Ni2+ 50% inhibition at 1 mM Phanerodontia chrysosporium
3.1.1.72 SDS 78.11% inhibition Phanerodontia chrysosporium
3.1.1.72 Triton X-100 61.42% inhibition Phanerodontia chrysosporium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.72 Ca2+ 9.6% activation at 1 mM Phanerodontia chrysosporium
3.1.1.72 Mg2+ 11.9% activation at 1 mM Phanerodontia chrysosporium
3.1.1.72 additional information no or poor effects by Fe3+, K+, and Zn2+ Phanerodontia chrysosporium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.72 37000
-
 x * 63000, recombinant His-tagged enzyme, SDS-PAGE, x * 55000, partially deglycosylated His-tagged enzyme, SDS-PAGE, x * 37000, about, sequence calculation Phanerodontia chrysosporium
3.1.1.72 55000
-
 x * 63000, recombinant His-tagged enzyme, SDS-PAGE, x * 55000, partially deglycosylated His-tagged enzyme, SDS-PAGE, x * 37000, about, sequence calculation Phanerodontia chrysosporium
3.1.1.72 63000
-
 x * 63000, recombinant His-tagged enzyme, SDS-PAGE, x * 55000, partially deglycosylated His-tagged enzyme, SDS-PAGE, x * 37000, about, sequence calculation Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.72 Phanerodontia chrysosporium H2ESB9 gene axe2
-
3.1.1.72 Phanerodontia chrysosporium BKM-F-1767 H2ESB9 gene axe2
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.72 glycoprotein the recombinant His-tagged enzyme is hyperglycosylated and contains two potential N-glycosylation sites and several O-glycosylation sites Phanerodontia chrysosporium

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.72 recombinant His-tagged enzyme from Pichia pastoris by nickel affinity chromatography and dialysis Phanerodontia chrysosporium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.72 39.86
-
 purified recombinant enzyme, pH 7.0, 30°C Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.72 4-nitrophenyl acetate + H2O
-
 Phanerodontia chrysosporium 4-nitrophenol + acetate
-
 ?
3.1.1.72 4-nitrophenyl acetate + H2O
-
 Phanerodontia chrysosporium BKM-F-1767 4-nitrophenol + acetate
-
 ?
3.1.1.72 hardwood acetyl glucuronoxylan xylan is composed of a beta-1,4-xylosyl backbone with arabinofuranose, glucuronic acid, methylglucuronic acid and acetyl side groups. Approximately 60-70% of xylose residues in hardwood xylan are substituted with acetyl groups in the O-2 or O-3 position Phanerodontia chrysosporium ?
-
 ?
3.1.1.72 hardwood acetyl glucuronoxylan xylan is composed of a beta-1,4-xylosyl backbone with arabinofuranose, glucuronic acid, methylglucuronic acid and acetyl side groups. Approximately 60-70% of xylose residues in hardwood xylan are substituted with acetyl groups in the O-2 or O-3 position Phanerodontia chrysosporium BKM-F-1767 ?
-
 ?
3.1.1.72 additional information the enzyme Axe2 also exhibits a xylanase activity, EC 3.2.1.8. Synergistic action of the enzyme with rPcXynC on birchwood xylan, beechwood xylan and wheat arabinoxylan Phanerodontia chrysosporium ?
-
 ?
3.1.1.72 additional information the enzyme Axe2 also exhibits a xylanase activity, EC 3.2.1.8. Synergistic action of the enzyme with rPcXynC on birchwood xylan, beechwood xylan and wheat arabinoxylan Phanerodontia chrysosporium BKM-F-1767 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.72 ? x * 63000, recombinant His-tagged enzyme, SDS-PAGE, x * 55000, partially deglycosylated His-tagged enzyme, SDS-PAGE, x * 37000, about, sequence calculation Phanerodontia chrysosporium

Synonyms

EC Number Synonyms Comment Organism
3.1.1.72 acetyl xylan esterase
-
 Phanerodontia chrysosporium
3.1.1.72 acetyl xylan esterase 2
-
 Phanerodontia chrysosporium
3.1.1.72 PcAxe2
-
 Phanerodontia chrysosporium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.72 30 35
-
 Phanerodontia chrysosporium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.72 10 45 activity range, overview Phanerodontia chrysosporium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.72 30
-
 purified recombinant enzyme, pH 7.0, stable up to Phanerodontia chrysosporium
3.1.1.72 38
-
 about, purified recombinant enzyme, pH 7.0, 50% reduced activity Phanerodontia chrysosporium
3.1.1.72 50
-
 purified recombinant enzyme, pH 7.0, 20% activity remaining Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.72 7
-
-
 Phanerodontia chrysosporium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.72 6 8.5 activity range, overview Phanerodontia chrysosporium

General Information

EC Number General Information Comment Organism
3.1.1.72 metabolism white rot fungus Phanerochaete chrysosporium is capable of degrading woody biomass by enzymatic processes. Biomass degradation by this fungus is accomplished through a complex mixture of cellulases, hemicellulases and ligninases. Its putative xylan-degrading system involves xylanases, acetyl xylan esterases, alpha-L-arabinofuranosidases, and glucuronyl esterases (alpha-D-glucuronidase) Phanerodontia chrysosporium