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Literature summary extracted from

  • Takahashi, Y.; Moiseyev, G.; Ma, J.X.
    Identification of key residues determining isomerohydrolase activity of human RPE65 (2014), J. Biol. Chem., 289, 6743-26751.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.64 recombinant expression of wild-type and mutant enzymes in HEK-293A-LRAT cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.64 C106Y site-directed mutagenesis, the mutant exhibits 16% reduced activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 C106Y/K297G site-directed mutagenesis, the mutant exhibits 26% reduced activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 C330T site-directed mutagenesis, the mutant exhibits 27% reduced activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 I220M site-directed mutagenesis, the mutant exhibits 38% reduced activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 K297G site-directed mutagenesis, the mutant exhibits 1.7fold increased activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 K297G/L510M site-directed mutagenesis, the mutant exhibits 45% increased activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 K297G/N302I site-directed mutagenesis, the mutant exhibits 7.7% increased activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 K297G/S533A site-directed mutagenesis, the mutant exhibits 18% increased activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 L510M site-directed mutagenesis, the mutant exhibits 29% reduced activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 additional information mutant enzymes' recombinant expression levels, overview Homo sapiens
3.1.1.64 N170K site-directed mutagenesis, the mutant exhibits 1.6fold increased activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 N170K/C330T site-directed mutagenesis, the mutant exhibits 15% reduced activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 N170K/I220M site-directed mutagenesis, the mutant exhibits 69% reduced activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 N170K/K297G site-directed mutagenesis, the mutant exhibits 67% increased activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 N170K/Q497P site-directed mutagenesis, the mutant exhibits 16% increased activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 N302I site-directed mutagenesis, the mutant exhibits 25% increased activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 Q497P site-directed mutagenesis, the mutant exhibits 26% reduced activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 S533A site-directed mutagenesis, the mutant exhibits 23% increased activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 T39R site-directed mutagenesis, the mutant exhibits 22% increased activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 T39R/N170K site-directed mutagenesis, the mutant exhibits 39% increased activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 T39R/N170K/C330T site-directed mutagenesis, the mutant exhibits 8.4% increased activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 T39R/N170K/C330T/Q497P site-directed mutagenesis, the mutant exhibits 23% reduced activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 T39R/N170K/I220M site-directed mutagenesis, the mutant exhibits 10% increased activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 T39R/N170K/I220M/Q497P site-directed mutagenesis, the mutant exhibits 8.6% increased activity compared to the wild-type enzyme Homo sapiens
3.1.1.64 T39R/N170K/Q497P site-directed mutagenesis, the mutant exhibits 11% increased activity compared to the wild-type enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.64 0.00104
-
 an all-trans-retinyl ester pH and temperature not specified in the publication Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.64 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.64 an all-trans-retinyl ester + H2O the enzyme generates predominantly 11-cis-retinol (72.2%) and a minor amount of 13-cis-retinol (27.8%), from all-trans-retinyl ester Homo sapiens 11-cis-retinol + a fatty acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.64 isomerohydrolase
-
 Homo sapiens
3.1.1.64 retinoid isomerohydrolase
-
 Homo sapiens
3.1.1.64 Rpe65
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
3.1.1.64 metabolism the enzyme catalyzes the key reaction in the retinoid visual cycle Homo sapiens