EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.5.B1 | dATP | both dATP and dGTP are co-activators for hydrolysis of dTTP, dATP might bind at the secondary allosteric site | Enterococcus faecalis | |
3.1.5.B1 | dCTP | allosteric activator, less potent than dGTP and dATP | Enterococcus faecalis | |
3.1.5.B1 | dGTP | the tetrameric enzyme EF1143 contains four additional secondary allosteric sites adjacent to the previously identified dGTP-binding primary regulatory sites. dGTP binding to the first allosteric site, with nanomolar affinity, is a prerequisite for substrate docking and hydrolysis. Then, the presence of a particular dNTP in the second site either enhances or inhibits the dNTPase activity | Enterococcus faecalis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.5.B1 | crystal structure in complex with dGTP and dTTP, to 2.35 A resolution. The tetrameric enzyme EF1143 contains four additional secondary allosteric sites adjacent to the previously identified dGTP-binding primary regulatory sites. dGTP binding to the first allosteric site, with nanomolar affinity, is a prerequisite for substrate docking and hydrolysis. Then, the presence of a particular dNTP in the second site either enhances or inhibits the dNTPase activity | Enterococcus faecalis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.5.B1 | H114A | no residual activity | Enterococcus faecalis |
3.1.5.B1 | H129A | less than 5% residual activity | Enterococcus faecalis |
3.1.5.B1 | K14A | less than 5% residual activity | Enterococcus faecalis |
3.1.5.B1 | K330A | no residual activity | Enterococcus faecalis |
3.1.5.B1 | N36A | no residual activity | Enterococcus faecalis |
3.1.5.B1 | Q241A | about 10% residual activity | Enterococcus faecalis |
3.1.5.B1 | Q41A | less than 5% residual activity | Enterococcus faecalis |
3.1.5.B1 | R17A | no residual activity | Enterococcus faecalis |
3.1.5.B1 | R17Q | about 5% residual activity | Enterococcus faecalis |
3.1.5.B1 | R206A/R209A | no residual activity | Enterococcus faecalis |
3.1.5.B1 | R206Q | about 30% residual activity | Enterococcus faecalis |
3.1.5.B1 | R209S | about 70% residual activity | Enterococcus faecalis |
3.1.5.B1 | R326A | about 40% residual activity | Enterococcus faecalis |
3.1.5.B1 | Y199A | no residual activity | Enterococcus faecalis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.5.B1 | Enterococcus faecalis | Q836G9 | - |
- |
3.1.5.B1 | Enterococcus faecalis ATCC 700802 | Q836G9 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.5.B1 | additional information | enzyme EF1143 fails to hydrolyze any of the dNTPs when a sole dNTP is present in the reaction mixture. Among the four dNTPs, only dGTP can function as an activator of dATP and dCTP hydrolysis. dGTP hydrolysis is clearly enhanced by the presence of dATP or dCTP, but not dTTP, with dATP being the more potent activator. Further, dTTP hydrolysis is virtually nonexistent when any one other dNTP is combined with dTTP in the reaction mixture. With all four dNTPs present at equal concentrations, the decreasing order of dNTP hydrolysis is dGTP, dCTP, dATP, dTTP | Enterococcus faecalis | ? | - |
? | |
3.1.5.B1 | additional information | enzyme EF1143 fails to hydrolyze any of the dNTPs when a sole dNTP is present in the reaction mixture. Among the four dNTPs, only dGTP can function as an activator of dATP and dCTP hydrolysis. dGTP hydrolysis is clearly enhanced by the presence of dATP or dCTP, but not dTTP, with dATP being the more potent activator. Further, dTTP hydrolysis is virtually nonexistent when any one other dNTP is combined with dTTP in the reaction mixture. With all four dNTPs present at equal concentrations, the decreasing order of dNTP hydrolysis is dGTP, dCTP, dATP, dTTP | Enterococcus faecalis ATCC 700802 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.5.B1 | EF_1143 | - |
Enterococcus faecalis |
3.1.5.B1 | HD domain protein | - |
Enterococcus faecalis |