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Literature summary extracted from

  • Vorontsov, I.I.; Wu, Y.; DeLucia, M.; Minasov, G.; Mehrens, J.; Shuvalova, L.; Anderson, W.F.; Ahn, J.
    Mechanisms of allosteric activation and inhibition of the deoxyribonucleoside triphosphate triphosphohydrolase from Enterococcus faecalis (2014), J. Biol. Chem., 289, 2815-2824.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.5.B1 dATP both dATP and dGTP are co-activators for hydrolysis of dTTP, dATP might bind at the secondary allosteric site Enterococcus faecalis
3.1.5.B1 dCTP allosteric activator, less potent than dGTP and dATP Enterococcus faecalis
3.1.5.B1 dGTP the tetrameric enzyme EF1143 contains four additional secondary allosteric sites adjacent to the previously identified dGTP-binding primary regulatory sites. dGTP binding to the first allosteric site, with nanomolar affinity, is a prerequisite for substrate docking and hydrolysis. Then, the presence of a particular dNTP in the second site either enhances or inhibits the dNTPase activity Enterococcus faecalis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.5.B1 crystal structure in complex with dGTP and dTTP, to 2.35 A resolution. The tetrameric enzyme EF1143 contains four additional secondary allosteric sites adjacent to the previously identified dGTP-binding primary regulatory sites. dGTP binding to the first allosteric site, with nanomolar affinity, is a prerequisite for substrate docking and hydrolysis. Then, the presence of a particular dNTP in the second site either enhances or inhibits the dNTPase activity Enterococcus faecalis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.5.B1 H114A no residual activity Enterococcus faecalis
3.1.5.B1 H129A less than 5% residual activity Enterococcus faecalis
3.1.5.B1 K14A less than 5% residual activity Enterococcus faecalis
3.1.5.B1 K330A no residual activity Enterococcus faecalis
3.1.5.B1 N36A no residual activity Enterococcus faecalis
3.1.5.B1 Q241A about 10% residual activity Enterococcus faecalis
3.1.5.B1 Q41A less than 5% residual activity Enterococcus faecalis
3.1.5.B1 R17A no residual activity Enterococcus faecalis
3.1.5.B1 R17Q about 5% residual activity Enterococcus faecalis
3.1.5.B1 R206A/R209A no residual activity Enterococcus faecalis
3.1.5.B1 R206Q about 30% residual activity Enterococcus faecalis
3.1.5.B1 R209S about 70% residual activity Enterococcus faecalis
3.1.5.B1 R326A about 40% residual activity Enterococcus faecalis
3.1.5.B1 Y199A no residual activity Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
3.1.5.B1 Enterococcus faecalis Q836G9
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3.1.5.B1 Enterococcus faecalis ATCC 700802 Q836G9
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-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.5.B1 additional information enzyme EF1143 fails to hydrolyze any of the dNTPs when a sole dNTP is present in the reaction mixture. Among the four dNTPs, only dGTP can function as an activator of dATP and dCTP hydrolysis. dGTP hydrolysis is clearly enhanced by the presence of dATP or dCTP, but not dTTP, with dATP being the more potent activator. Further, dTTP hydrolysis is virtually nonexistent when any one other dNTP is combined with dTTP in the reaction mixture. With all four dNTPs present at equal concentrations, the decreasing order of dNTP hydrolysis is dGTP, dCTP, dATP, dTTP Enterococcus faecalis ?
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?
3.1.5.B1 additional information enzyme EF1143 fails to hydrolyze any of the dNTPs when a sole dNTP is present in the reaction mixture. Among the four dNTPs, only dGTP can function as an activator of dATP and dCTP hydrolysis. dGTP hydrolysis is clearly enhanced by the presence of dATP or dCTP, but not dTTP, with dATP being the more potent activator. Further, dTTP hydrolysis is virtually nonexistent when any one other dNTP is combined with dTTP in the reaction mixture. With all four dNTPs present at equal concentrations, the decreasing order of dNTP hydrolysis is dGTP, dCTP, dATP, dTTP Enterococcus faecalis ATCC 700802 ?
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?

Synonyms

EC Number Synonyms Comment Organism
3.1.5.B1 EF_1143
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Enterococcus faecalis
3.1.5.B1 HD domain protein
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Enterococcus faecalis