Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zheng, Y.; Brash, A.R.
    Dioxygenase activity of epidermal lipoxygenase-3 unveiled: typical and atypical features of its catalytic activity with natural and synthetic polyunsaturated fatty acids (2010), J. Biol. Chem., 285, 39866-39875.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.58 13S-HPODE
-
 Homo sapiens
4.2.1.152 (13S)-HPODE
-
 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.58
-
 Homo sapiens
4.2.1.152
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.58 A451G the mutant reacts well with arachidonic acid even in the absence of fatty acid hydroperoxides Homo sapiens
4.2.1.152 A451G unlike wild type enzyme, the mutant reacts well with arachidonic acid even in the absence of fatty acid hydroperoxides, yet only after an unusually long lag phase of about 20 min Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.58 0.05
-
 9E,11Z,14Z-20:3omega6 at pH 7.5 and 22°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.58 Fe2+ contains Fe2+ Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.58 Homo sapiens
-
-
-
4.2.1.152 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.58
-
 Homo sapiens
4.2.1.152
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.58 (9E,11Z,14Z)-icosa-9,11,14-trienoic acid + O2 good substrate Homo sapiens (9S,11Z,14Z)-9-hydroperoxyicosa-11,14-dienoic acid
-
 ?
1.13.11.58 AA/Lyso-PA + O2
-
 Homo sapiens 15-HPETE/lyso-PA + 13-HPETE/lyso-PA + 15-HPETE/lyso-PA + 11-HPETE/lyso-PA + 5-HPETE/lyso-PA 36%, 22%, 21% and 13% yield, respectively ?
1.13.11.58 arachidonic acid + O2
-
 Homo sapiens 5-HPETE + 7-HPETE + 9-HPETE 22%, 25% and 29% yield, respectively ?
4.2.1.152 (9E,11Z,14Z)-20:3omega6 good substrate Homo sapiens (9S)-hydroperoxy-(10E,12E,14Z)-eicosatrienoic acid + H2O
-
 ?
4.2.1.152 arachidonic acid + O2 in the absence of fatty acid hydroperoxides, the enzyme shows only weak and slow activity with arachidonic acid Homo sapiens 5-HPETE + 7-HPETE + 9-HPETE 22%, 25%, and 29% yield, respectively ?
4.2.1.152 arachidonoyl-lysophosphatidic acid
-
 Homo sapiens 15-HPETE-lysophosphatidic acid + 13-HPETE-lysophosphatidic acid + 11HPETE-lysophosphatidic acid + 5-HPETE-lysophosphatidic acid + H2O 36%, 22%, 21%, and 13% yield, respectively ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.58 eLOX3
-
 Homo sapiens
1.13.11.58 epidermal lipoxygenase-3
-
 Homo sapiens
4.2.1.152 eLOX3
-
 Homo sapiens
4.2.1.152 epidermal lipoxygenase-3
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.58 1.6
-
 9E,11Z,14Z-20:3omega6 at pH 7.5 and 22°C Homo sapiens