EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.233 | expressed in Escherichia coli BL21(DE3) cells | Streptomyces coelicolor |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.233 | C138A | inactive with malonyl-CoA | Streptomyces coelicolor |
2.3.1.233 | C138S | inactive with malonyl-CoA | Streptomyces coelicolor |
2.3.1.233 | Y224A | the mutant shows 3% activity with malonyl-CoA compared to the wild type enzyme | Streptomyces coelicolor |
2.3.1.233 | Y224C | the mutant shows 50% activity with malonyl-CoA compared to the wild type enzyme | Streptomyces coelicolor |
2.3.1.233 | Y224F | the mutant shows 70% activity with malonyl-CoA compared to the wild type enzyme | Streptomyces coelicolor |
2.3.1.233 | Y224G | inactive with malonyl-CoA but capable of accepting acetoacetyl-CoA and acetyl-CoA | Streptomyces coelicolor |
2.3.1.233 | Y224H | inactive with malonyl-CoA but capable of accepting acetoacetyl-CoA and acetyl-CoA | Streptomyces coelicolor |
2.3.1.233 | Y224L | the mutant shows 65% activity with malonyl-CoA compared to the wild type enzyme | Streptomyces coelicolor |
2.3.1.233 | Y224L | the mutant shows strongly reduced catalytic efficiency with malonyl-CoA compared to the wild type enzyme | Streptomyces griseus |
2.3.1.233 | Y224M | the mutant shows 15% activity with malonyl-CoA compared to the wild type enzyme | Streptomyces coelicolor |
2.3.1.233 | Y224S | inactive with malonyl-CoA but capable of accepting acetoacetyl-CoA and acetyl-CoA | Streptomyces coelicolor |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.233 | 0.0011 | - |
malonyl-CoA | wild type enzyme, at pH 7.4 and 30°C | Streptomyces griseus | |
2.3.1.233 | 0.0039 | - |
malonyl-CoA | wild type enzyme, at pH 7.4 and 30°C | Streptomyces coelicolor | |
2.3.1.233 | 0.0095 | - |
malonyl-CoA | mutant enzyme Y224F, at pH 7.4 and 30°C | Streptomyces coelicolor | |
2.3.1.233 | 0.0111 | - |
malonyl-CoA | mutant enzyme Y224L, at pH 7.4 and 30°C | Streptomyces griseus | |
2.3.1.233 | 0.012 | - |
malonyl-CoA | mutant enzyme Y224C, at pH 7.4 and 30°C | Streptomyces coelicolor | |
2.3.1.233 | 0.0171 | - |
malonyl-CoA | mutant enzyme Y224M, at pH 7.4 and 30°C | Streptomyces coelicolor | |
2.3.1.233 | 0.0234 | - |
malonyl-CoA | mutant enzyme Y224L, at pH 7.4 and 30°C | Streptomyces coelicolor |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.233 | 43000 | - |
x * 43000, SDS-PAGE | Streptomyces coelicolor |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.233 | 5 malonyl-CoA | Streptomyces coelicolor | - |
1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O | - |
? | |
2.3.1.233 | 5 malonyl-CoA | Streptomyces griseus | - |
1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O | - |
? | |
2.3.1.233 | 5 malonyl-CoA | Streptomyces coelicolor A3 (2) | - |
1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.233 | Streptomyces coelicolor | - |
- |
- |
2.3.1.233 | Streptomyces coelicolor A3 (2) | - |
- |
- |
2.3.1.233 | Streptomyces griseus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.233 | nickel-nitrilotriacetic acid-agarose column chromatography | Streptomyces coelicolor |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.233 | 5 malonyl-CoA | - |
Streptomyces coelicolor | 1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O | - |
? | |
2.3.1.233 | 5 malonyl-CoA | - |
Streptomyces griseus | 1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O | - |
? | |
2.3.1.233 | 5 malonyl-CoA | - |
Streptomyces coelicolor A3 (2) | 1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O | - |
? | |
2.3.1.233 | acetoacetyl-CoA | - |
Streptomyces griseus | 4-hydroxy-6-methyl-2-pyrone + CoA + CO2 + H2O | - |
? | |
2.3.1.233 | acetoacetyl-CoA + 2 malonyl-CoA | - |
Streptomyces coelicolor | 4-hydroxy-6-methyl-2-pyrone + CoA + CO2 + H2O | - |
? | |
2.3.1.233 | acetoacetyl-CoA + 2 malonyl-CoA | - |
Streptomyces coelicolor A3 (2) | 4-hydroxy-6-methyl-2-pyrone + CoA + CO2 + H2O | - |
? | |
2.3.1.233 | acetyl-CoA | - |
Streptomyces griseus | ? + CoA + CO2 + H2O | - |
? | |
2.3.1.233 | acetyl-CoA + 2 malonyl-CoA | - |
Streptomyces coelicolor | ? + CoA + CO2 + H2O | - |
? | |
2.3.1.233 | acetyl-CoA + 2 malonyl-CoA | - |
Streptomyces coelicolor A3 (2) | ? + CoA + CO2 + H2O | - |
? | |
2.3.1.233 | benzoyl-CoA | - |
Streptomyces griseus | ? + CoA + CO2 + H2O | - |
? | |
2.3.1.233 | benzoyl-CoA + 2 malonyl-CoA | - |
Streptomyces coelicolor | 4-hydroxy-6-phenyl-2H-pyran-2-one + CoA + CO2 + H2O | - |
? | |
2.3.1.233 | benzoyl-CoA + 2 malonyl-CoA | - |
Streptomyces coelicolor A3 (2) | 4-hydroxy-6-phenyl-2H-pyran-2-one + CoA + CO2 + H2O | - |
? | |
2.3.1.233 | hexanoyl-CoA | - |
Streptomyces griseus | ? + CoA + CO2 + H2O | - |
? | |
2.3.1.233 | hexanoyl-CoA + 2 malonyl-CoA | - |
Streptomyces coelicolor | 4-hydroxy-6-pentyl-2H-pyran-2-one + CoA + CO2 + H2O | - |
? | |
2.3.1.233 | hexanoyl-CoA + 2 malonyl-CoA | - |
Streptomyces coelicolor A3 (2) | 4-hydroxy-6-pentyl-2H-pyran-2-one + CoA + CO2 + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.233 | ? | x * 43000, SDS-PAGE | Streptomyces coelicolor |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.233 | RppA | - |
Streptomyces coelicolor |
2.3.1.233 | RppA | - |
Streptomyces griseus |
2.3.1.233 | THN synthase | - |
Streptomyces coelicolor |
2.3.1.233 | THN synthase | - |
Streptomyces griseus |
2.3.1.233 | THNS | - |
Streptomyces coelicolor |
2.3.1.233 | THNS | - |
Streptomyces griseus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.233 | 0.0003 | - |
malonyl-CoA | mutant enzyme Y224L, at pH 7.4 and 30°C | Streptomyces griseus | |
2.3.1.233 | 0.002 | - |
malonyl-CoA | mutant enzyme Y224M, at pH 7.4 and 30°C | Streptomyces coelicolor | |
2.3.1.233 | 0.004 | - |
malonyl-CoA | mutant enzyme Y224L, at pH 7.4 and 30°C | Streptomyces coelicolor | |
2.3.1.233 | 0.005 | - |
malonyl-CoA | mutant enzyme Y224C, at pH 7.4 and 30°C | Streptomyces coelicolor | |
2.3.1.233 | 0.005 | - |
malonyl-CoA | wild type enzyme, at pH 7.4 and 30°C | Streptomyces griseus | |
2.3.1.233 | 0.018 | - |
malonyl-CoA | mutant enzyme Y224F, at pH 7.4 and 30°C | Streptomyces coelicolor | |
2.3.1.233 | 0.022 | - |
malonyl-CoA | wild type enzyme, at pH 7.4 and 30°C | Streptomyces coelicolor |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.233 | 0.133 | - |
malonyl-CoA | mutant enzyme Y224M, at pH 7.4 and 30°C | Streptomyces coelicolor | |
2.3.1.233 | 0.175 | - |
malonyl-CoA | mutant enzyme Y224L, at pH 7.4 and 30°C | Streptomyces coelicolor | |
2.3.1.233 | 0.298 | - |
malonyl-CoA | mutant enzyme Y224L, at pH 7.4 and 30°C | Streptomyces griseus | |
2.3.1.233 | 0.396 | - |
malonyl-CoA | mutant enzyme Y224C, at pH 7.4 and 30°C | Streptomyces coelicolor | |
2.3.1.233 | 1.93 | - |
malonyl-CoA | mutant enzyme Y224F, at pH 7.4 and 30°C | Streptomyces coelicolor | |
2.3.1.233 | 5.74 | - |
malonyl-CoA | wild type enzyme, at pH 7.4 and 30°C | Streptomyces coelicolor | |
2.3.1.233 | 44.58 | - |
malonyl-CoA | wild type enzyme, at pH 7.4 and 30°C | Streptomyces griseus |