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Literature summary extracted from

  • Wang, L.; Ma, Z.; Du, F.; Wang, H.; Ng, T.B.
    Feruloyl esterase from the edible mushroom Panus giganteus: a potential dietary supplement (2014), J. Agric. Food Chem., 62, 7822-7827.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.73 Al3+ 40% inhibition at 5 mM Pleurotus giganteus
3.1.1.73 Ca2+ 60% inhibition at 5 mM Pleurotus giganteus
3.1.1.73 Cd2+ 60% inhibition at 5 mM Pleurotus giganteus
3.1.1.73 Cu2+ 40% inhibition at 5 mM Pleurotus giganteus
3.1.1.73 K+ 40% inhibition at 5 mM Pleurotus giganteus
3.1.1.73 Mg2+ 40% inhibition at 5 mM Pleurotus giganteus
3.1.1.73 Mn2+ 60% inhibition at 5 mM Pleurotus giganteus
3.1.1.73 Pb2+ 20% inhibition at 5 mM Pleurotus giganteus
3.1.1.73 Zn2+ 40% inhibition at 5 mM Pleurotus giganteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.36
-
 methyl ferulate pH 5.0, 37°C Pleurotus giganteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 61000
-
 x * 61000, SDS-PAGE Pleurotus giganteus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.73 additional information Pleurotus giganteus the single purified enzyme brings about the release of ferulic acid from wheat bran and corn bran, and the amount released from wheat bran rises to 51.9% (of the total amount) by the synergistic action of xylanase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Pleurotus giganteus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73 native enzyme 130.8fold from fruiting bodies by two different steps of anion exchange chromatography, and gel filtration Pleurotus giganteus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.73 fruit body fresh Pleurotus giganteus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 170
-
 purified enzyme, pH 5.0, 37°C Pleurotus giganteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 4-nitrophenyl acetate + H2O
-
 Pleurotus giganteus 4-nitrophenol + acetate
-
 ?
3.1.1.73 4-nitrophenyl butyrate + H2O
-
 Pleurotus giganteus 4-nitrophenol + butyrate
-
 ?
3.1.1.73 4-nitrophenyl caproate + H2O
-
 Pleurotus giganteus 4-nitrophenol + caproate
-
 ?
3.1.1.73 methyl 4-coumarate + H2O
-
 Pleurotus giganteus 4-coumarate + methanol
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Pleurotus giganteus caffeic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Pleurotus giganteus ferulate + methanol
-
 ?
3.1.1.73 additional information the single purified enzyme brings about the release of ferulic acid from wheat bran and corn bran, and the amount released from wheat bran rises to 51.9% (of the total amount) by the synergistic action of xylanase Pleurotus giganteus ?
-
 ?
3.1.1.73 additional information no activity with 4-nitrophenyl palmitate and 4-nitrophenyl stearate Pleurotus giganteus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 ? x * 61000, SDS-PAGE Pleurotus giganteus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FAE
-
 Pleurotus giganteus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 40
-
-
 Pleurotus giganteus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.73 20 90 68% of maximal activity at 20°C, 45% at 90°C, inactivation at 100°C Pleurotus giganteus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 100
-
 inactivation Pleurotus giganteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 4
-
-
 Pleurotus giganteus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 2 7 60% of maximal activity at pH 2.0, maximal activity at pH 4.0, very low activity at pH 7.0, inactive at pH 8.0 Pleurotus giganteus