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Literature summary extracted from

  • Navarro-Gonzalez, I.; Sanchez-Ferrer, A.; Garcia-Carmona, F.
    Molecular characterization of a novel arylesterase from the wine-associated acetic acid bacterium Gluconobacter oxidans 621H (2012), J. Agric. Food Chem., 60, 10789-10795.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.2 expression in Escherichia coli Gluconobacter oxydans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.2 diethyldicarbonate 0.5 mM, complete loss of activity Gluconobacter oxydans
3.1.1.2 eserine 0.5 mM, 42% residual activity Gluconobacter oxydans
3.1.1.2 paraoxon 0.5 mM, complete loss of activity Gluconobacter oxydans
3.1.1.2 Phenylglyoxal 0.5 mM, 77% residual activity Gluconobacter oxydans
3.1.1.2 pyridoxal 5'-phosphate 0.5 mM, 62% residual activity Gluconobacter oxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.2 0.0052
-
 3-naphthyl acetate pH 7.0, 25° Gluconobacter oxydans
3.1.1.2 0.0079
-
 2-naphthyl acetate pH 7.0, 25° Gluconobacter oxydans
3.1.1.2 0.0088
-
 3-naphthyl butanoate pH 7.0, 25° Gluconobacter oxydans
3.1.1.2 0.01
-
 phenyl acetate pH 7.0, 25° Gluconobacter oxydans
3.1.1.2 0.0128
-
 2-naphthyl butanoate pH 7.0, 25° Gluconobacter oxydans
3.1.1.2 0.0174
-
 4-nitrophenyl acetate pH 7.0, 25° Gluconobacter oxydans
3.1.1.2 0.174
-
 4-nitrophenyl butanoate pH 7.0, 25° Gluconobacter oxydans
3.1.1.2 7.2
-
 S-methyl thiobutanoate pH 8.0, 25° Gluconobacter oxydans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.2 35000
-
 2 * 35000, SDS-PAGE Gluconobacter oxydans
3.1.1.2 76800
-
 gel filtration Gluconobacter oxydans

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.2 Acetone 10% v/v, 93% residual activity Gluconobacter oxydans
3.1.1.2 acetonitrile 10% v/v, 95% residual activity Gluconobacter oxydans
3.1.1.2 dimethyl sulfoxide 10% v/v, 90% residual activity, 40% v/v, 45% residual activity Gluconobacter oxydans
3.1.1.2 Ethanol 10% v/v, 92% residual activity Gluconobacter oxydans
3.1.1.2 Methanol 10% v/v, 90% residual activity, 40% v/v, 57% residual activity Gluconobacter oxydans
3.1.1.2 propanol 10% v/v, 90% residual activity Gluconobacter oxydans

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.2 Gluconobacter oxydans Q5FSJ2
-
-
3.1.1.2 Gluconobacter oxydans 621H Q5FSJ2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.2
-
 Gluconobacter oxydans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.2 214
-
 pH 7.0, 25°C Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.2 2-naphthyl acetate + H2O
-
 Gluconobacter oxydans 2-naphthol + acetate
-
 ?
3.1.1.2 2-naphthyl acetate + H2O
-
 Gluconobacter oxydans 621H 2-naphthol + acetate
-
 ?
3.1.1.2 2-naphthyl butanoate + H2O
-
 Gluconobacter oxydans 2-naphthol + butanoate
-
 ?
3.1.1.2 3-naphthyl acetate + H2O
-
 Gluconobacter oxydans 3-naphthol + acetate
-
 ?
3.1.1.2 3-naphthyl butanoate + H2O
-
 Gluconobacter oxydans 3-naphthol + butanoate
-
 ?
3.1.1.2 4-nitrophenyl acetate + H2O
-
 Gluconobacter oxydans 4-nitrophenol + acetate
-
 ?
3.1.1.2 4-nitrophenyl acetate + H2O
-
 Gluconobacter oxydans 621H 4-nitrophenol + acetate
-
 ?
3.1.1.2 4-nitrophenyl butanoate + H2O
-
 Gluconobacter oxydans 4-nitrophenol + butanoate
-
 ?
3.1.1.2 4-nitrophenyl butanoate + H2O
-
 Gluconobacter oxydans 621H 4-nitrophenol + butanoate
-
 ?
3.1.1.2 additional information substrate specificity of this arylesterase is higher toward short chain pnitrophenyl esters (C2 to C4) and also toward aromatic esters, such as phenyl acetate. enzyme is a serine esterase, the catalytic triad is composed of residues Ser163, Asp263, and His293 in the active site Gluconobacter oxydans ?
-
 ?
3.1.1.2 additional information substrate specificity of this arylesterase is higher toward short chain pnitrophenyl esters (C2 to C4) and also toward aromatic esters, such as phenyl acetate. enzyme is a serine esterase, the catalytic triad is composed of residues Ser163, Asp263, and His293 in the active site Gluconobacter oxydans 621H ?
-
 ?
3.1.1.2 phenyl acetate + H2O
-
 Gluconobacter oxydans phenol + acetate
-
 ?
3.1.1.2 phenyl acetate + H2O
-
 Gluconobacter oxydans 621H phenol + acetate
-
 ?
3.1.1.2 S-methyl thiobutanoate + H2O
-
 Gluconobacter oxydans methanol + thiobutanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.2 dimer 2 * 35000, SDS-PAGE Gluconobacter oxydans

Synonyms

EC Number Synonyms Comment Organism
3.1.1.2 Est0881
-
 Gluconobacter oxydans
3.1.1.2 gox0881
-
 Gluconobacter oxydans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.2 40
-
-
 Gluconobacter oxydans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.2 7
-
 2-naphthyl butanoate pH 7.0, 25° Gluconobacter oxydans
3.1.1.2 16.4
-
 3-naphthyl butanoate pH 7.0, 25° Gluconobacter oxydans
3.1.1.2 20
-
 S-methyl thiobutanoate pH 8.0, 25° Gluconobacter oxydans
3.1.1.2 55.2
-
 2-naphthyl acetate pH 7.0, 25° Gluconobacter oxydans
3.1.1.2 97.2
-
 4-nitrophenyl butanoate pH 7.0, 25° Gluconobacter oxydans
3.1.1.2 99
-
 3-naphthyl acetate pH 7.0, 25° Gluconobacter oxydans
3.1.1.2 185
-
 4-nitrophenyl acetate pH 7.0, 25° Gluconobacter oxydans
3.1.1.2 231
-
 phenyl acetate pH 7.0, 25° Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.2 7
-
-
 Gluconobacter oxydans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.2 5 8
-
 Gluconobacter oxydans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.2 2.8
-
 S-methyl thiobutanoate pH 8.0, 25° Gluconobacter oxydans
3.1.1.2 540
-
 2-naphthyl butanoate pH 7.0, 25° Gluconobacter oxydans
3.1.1.2 560
-
 4-nitrophenyl butanoate pH 7.0, 25° Gluconobacter oxydans
3.1.1.2 1900
-
 3-naphthyl butanoate pH 7.0, 25° Gluconobacter oxydans
3.1.1.2 6900
-
 2-naphthyl acetate pH 7.0, 25° Gluconobacter oxydans
3.1.1.2 10600
-
 4-nitrophenyl acetate pH 7.0, 25° Gluconobacter oxydans
3.1.1.2 19100
-
 3-naphthyl acetate pH 7.0, 25° Gluconobacter oxydans
3.1.1.2 23100
-
 phenyl acetate pH 7.0, 25° Gluconobacter oxydans