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Literature summary extracted from
- Active site characterization and molecular cloning of Tenebrio molitor midgut trehalase and comments on their insect homologs (2013), Insect Biochem. Mol. Biol., 43, 768-780.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.28 | - |
Tenebrio molitor |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.28 | amygdalin | i.e. glucose-beta-1,6-glucose-beta-mandelonitrile, linear competitive inhibitor | Tenebrio molitor |  |
| 3.2.1.28 | diethyldicarbonate | the compound modifies a His residue that results in a less active enzyme. After 40 min with 40 mM diethylpyrocarbonate at 30°C pH 6.0, trehalase activity decreases to about 50% of the initial activity | Tenebrio molitor | |
| 3.2.1.28 | glucono-delta-lactone | linear competitive inhibitor | Tenebrio molitor | |
| 3.2.1.28 | mandelonitrile | mixed-type non-competitive inhibitor | Tenebrio molitor | |
| 3.2.1.28 | methyl-alpha-D-mannoside | linear competitive inhibitor | Tenebrio molitor | |
| 3.2.1.28 | additional information | EDTA, polyaminopropyl biguanide, tetranitromethane, N-bromosuccinamide, gentiobiose (glucose-beta-1,6-glucose), methyl alpha-D-glucoside, 1,10-phenanthroline, and Tris (pH 6.0) do not inhibit the trehalase | Tenebrio molitor | |
| 3.2.1.28 | phloretin | linear competitive inhibitor | Tenebrio molitor | |
| 3.2.1.28 | phlorizin | i.e. glucose-phloretin, linear competitive inhibitor | Tenebrio molitor | |
| 3.2.1.28 | prunasin | i.e glucose-beta-mandelonitrile, linear competitive inhibitor | Tenebrio molitor | |
| 3.2.1.28 | Salicin | linear competitive inhibitor | Tenebrio molitor | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.28 | 0.43 | - |
alpha,alpha-trehalose | at pH 6.0 and 30°C | Tenebrio molitor | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.28 | soluble | - |
Tenebrio molitor | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 58000 | - |
x * 58000, SDS-PAGE | Tenebrio molitor |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.28 | alpha,alpha-trehalose + H2O | Tenebrio molitor | - |
beta-D-glucose + alpha-D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.28 | Tenebrio molitor | P32359 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.28 | Econo-Pac methyl hydrophobic interaction column chromatography, Resource Q column chromatography, and Superdex 75 gel filtration | Tenebrio molitor |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.28 | epithelium | - |
Tenebrio molitor | - |
| 3.2.1.28 | midgut | - |
Tenebrio molitor | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 0.226 | - |
supernatant, at pH 6.0 and 30°C | Tenebrio molitor |
| 3.2.1.28 | 16.67 | - |
after 74fold purification, at pH 6.0 and 30°C | Tenebrio molitor |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.28 | alpha,alpha-trehalose + H2O | - |
Tenebrio molitor | beta-D-glucose + alpha-D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.28 | ? | x * 58000, SDS-PAGE | Tenebrio molitor |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.28 | glucose alpha-1,1-glucose hydrolase | - |
Tenebrio molitor |
| 3.2.1.28 | TRE1 | - |
Tenebrio molitor |
| 3.2.1.28 | trehalase | - |
Tenebrio molitor |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.28 | 5.3 | - |
- |
Tenebrio molitor |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.28 | 0.008 | - |
phloretin | at pH 6.0 and 30°C | Tenebrio molitor | |
| 3.2.1.28 | 0.043 | - |
methyl-alpha-D-mannoside | at pH 6.0 and 30°C | Tenebrio molitor | |
| 3.2.1.28 | 0.22 | - |
amygdalin | at pH 6.0 and 30°C | Tenebrio molitor | |
| 3.2.1.28 | 0.43 | - |
prunasin | at pH 6.0 and 30°C | Tenebrio molitor | |
| 3.2.1.28 | 0.5 | - |
phlorizin | at pH 6.0 and 30°C | Tenebrio molitor | |
| 3.2.1.28 | 1.4 | - |
glucono-delta-lactone | at pH 6.0 and 30°C | Tenebrio molitor | |
| 3.2.1.28 | 190 | - |
Salicin | at pH 6.0 and 30°C | Tenebrio molitor | |
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