EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.73 | acetone | strong inhibition | Pleurotus eryngii | |
3.1.1.73 | acetonitrile | strong inhibition | Pleurotus eryngii | |
3.1.1.73 | Co2+ | inhibitory at 5 mM | Pleurotus eryngii | |
3.1.1.73 | diethyl ether | strong inhibition | Pleurotus eryngii | |
3.1.1.73 | DMF | strong inhibition | Pleurotus eryngii | |
3.1.1.73 | DMSO | strong inhibition | Pleurotus eryngii | |
3.1.1.73 | ethanol | strong inhibition | Pleurotus eryngii | |
3.1.1.73 | Hg2+ | inhibitory at 5 mM | Pleurotus eryngii | |
3.1.1.73 | isobutyl alcohol | strong inhibition | Pleurotus eryngii | |
3.1.1.73 | Li+ | inhibitory at 5 mM | Pleurotus eryngii | |
3.1.1.73 | methanol | complete inhibition | Pleurotus eryngii | |
3.1.1.73 | Mg2+ | inhibitory at 5 mM | Pleurotus eryngii | |
3.1.1.73 | Mn2+ | inhibitory at 5 mM | Pleurotus eryngii | |
3.1.1.73 | additional information | no inhibition by EDTA | Pleurotus eryngii | |
3.1.1.73 | n-heptane | - |
Pleurotus eryngii | |
3.1.1.73 | n-hexane | - |
Pleurotus eryngii | |
3.1.1.73 | PMSF | 50-100% inhibition at 1 mM dependent on the buffer system | Pleurotus eryngii | |
3.1.1.73 | SDS | 95% inhibition at 5 mM | Pleurotus eryngii | |
3.1.1.73 | tert-butyl methyl ether | strong inhibition | Pleurotus eryngii | |
3.1.1.73 | Toluene | strong inhibition | Pleurotus eryngii | |
3.1.1.73 | Zn2+ | inhibitory at 5 mM | Pleurotus eryngii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.73 | 0.15 | - |
methyl ferulate | pH 5.0, 37°C | Pleurotus eryngii |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.73 | extracellular | - |
Pleurotus eryngii | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.73 | Ca2+ | addition of 50 mM increases the initial activity by 10-20% | Pleurotus eryngii | |
3.1.1.73 | K+ | addition of 50 mM increases the initial activity by 10-20% | Pleurotus eryngii | |
3.1.1.73 | Li+ | addition of 50 mM increases the initial activity by 10-20% | Pleurotus eryngii | |
3.1.1.73 | Mg2+ | addition of 50 mM increases the initial activity by 10-20% | Pleurotus eryngii | |
3.1.1.73 | additional information | no or poor effects by Fe2+, Fe3+, Cu2+, Ni2+, Ca2+, K+ at 5 mM | Pleurotus eryngii | |
3.1.1.73 | NaCl | in the presence of 3 M activity of the enzyme increases by 28% | Pleurotus eryngii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 59200 | - |
1 * 67000, SDS-PAGE, 1 * 59200, about, sequence calculation | Pleurotus eryngii |
3.1.1.73 | 67000 | - |
gel filtration | Pleurotus eryngii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.73 | additional information | Pleurotus eryngii | the enzyme alone releases only little ferulic acid from destarched wheat bran, whereas after addition of Trichoderma viride xylanase the concentration of ferulic acid increases more than 20fold | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.73 | Pleurotus eryngii | V6BP73 | type A feruloyl esterase | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.1.73 | glycoprotein | four potential N-glycosylation sites at positions 132, 360, 396, and 521 are identified and the presence of at least one glycopeptide with mainly high mannose type glycans by ESI-MS/MS confirmed | Pleurotus eryngii |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.73 | native extracellular enzyme by ultrafiltration, cation exchange and hydrophobic interaction chromatography, and gel filtration to homogeneity | Pleurotus eryngii |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.73 | ethyl 4-hydroxybenzoate + H2O | low activity | Pleurotus eryngii | ethanol + 4-hydroxybenzoate | - |
? | |
3.1.1.73 | ethyl ferulate + H2O | - |
Pleurotus eryngii | ferulate + ethanol | - |
? | |
3.1.1.73 | gallic acid + H2O | low activity | Pleurotus eryngii | ? | - |
? | |
3.1.1.73 | methyl 4-coumarate + H2O | - |
Pleurotus eryngii | 4-coumarate + methanol | - |
? | |
3.1.1.73 | methyl 4-hydroxybenzoate + H2O | low activity | Pleurotus eryngii | methanol + 4-hydroxybenzoate | - |
? | |
3.1.1.73 | methyl ferulate + H2O | - |
Pleurotus eryngii | ferulate + methanol | - |
? | |
3.1.1.73 | methyl sinapinate + H2O | - |
Pleurotus eryngii | sinapate + methanol | - |
? | |
3.1.1.73 | additional information | the enzyme alone releases only little ferulic acid from destarched wheat bran, whereas after addition of Trichoderma viride xylanase the concentration of ferulic acid increases more than 20fold | Pleurotus eryngii | ? | - |
? | |
3.1.1.73 | propyl 4-hydroxybenzoate + H2O | low activity | Pleurotus eryngii | propanol + 4-hydroxybenzoate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.73 | monomer | 1 * 67000, SDS-PAGE, 1 * 59200, about, sequence calculation | Pleurotus eryngii |
3.1.1.73 | More | peptide mapping, overview | Pleurotus eryngii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.73 | FAE | - |
Pleurotus eryngii |
3.1.1.73 | PeFaeA | - |
Pleurotus eryngii |
3.1.1.73 | type A FAE | - |
Pleurotus eryngii |
3.1.1.73 | type A feruloyl esterase | - |
Pleurotus eryngii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 50 | - |
- |
Pleurotus eryngii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.73 | 0.85 | - |
methyl ferulate | pH 5.0, 37°C | Pleurotus eryngii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 5 | - |
- |
Pleurotus eryngii |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.73 | 3.5 | 7 | activity range with 50% of maximal activity within this range, rapid decrease in activity above pH 8.0 | Pleurotus eryngii |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.1.73 | Pleurotus eryngii | isoelectric focussing | - |
5.2 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.1.73 | additional information | the catalytic triad of Ser-Glu-His is unique among feruloyl esterases, the enzyme has a G-X-S-X-G motif | Pleurotus eryngii |