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Literature summary extracted from

  • Bagheri, A.; Khodarahmi, R.; Mostafaie, A.
    Purification and biochemical characterisation of glucoamylase from a newly isolated Aspergillus niger: relation to starch processing (2014), Food Chem., 161, 270-278.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.3 Al3+ 5 mM, about 30% residual activity Aspergillus niger
3.2.1.3 Fe3+ 5 mM, about 40% residual activity Aspergillus niger
3.2.1.3 Hg2+ 5 mM, about 30% residual activity Aspergillus niger
3.2.1.3 additional information not inhibitory at 5 mM: Ag2+, Ca2+, Zn2+, Mg2+ and Cd2+ and EDTA Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 additional information
-
 starch Km value is 0.33 mg/ml, pH 5.0, 40°C Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 57151
-
 x * 62000, SDS-PAGE, x * 57151, mass spectrometry Aspergillus niger
3.2.1.3 62000
-
 x * 62000, SDS-PAGE, x * 57151, mass spectrometry Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3
-
 Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 95
-
 pH 5.0, 40°C Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Aspergillus niger D-glucose + 4-nitrophenol
-
 ?
3.2.1.3 starch + H2O
-
 Aspergillus niger D-glucose + ? sole product ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 ? x * 62000, SDS-PAGE, x * 57151, mass spectrometry Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 70
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 60
-
 half-life 20-25 min Aspergillus niger
3.2.1.3 80
-
 half-life less than 3 min Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.3 158.3
-
 starch pH 5.0, 40°C Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 5
-
-
 Aspergillus niger

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.3 Aspergillus niger isoelectric focusing
-
 4.4