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Literature summary extracted from

  • Zhao, T.; No, d.a..S.; Kim, B.H.; Garcia, H.S.; Kim, Y.; Kim, I.H.
    Immobilized phospholipase A1-catalyzed modification of phosphatidylcholine with n-3 polyunsaturated fatty acid (2014), Food Chem., 157, 132-140.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.32 additional information enzyme immobilization using Lewatit VP OC 1600 as immobilization carrier, method optimization, n-3 polyunsaturated fatty acids-enriched phosphatidylcholine is successfully produced with fatty acid from fish oil and phosphatidylcholine from soybean by immobilized phospholipase A1-catalyzed acidolysis, best at ratio of fatty acid to phophatidylcholine of 8:1 mol/mol, overview. The yield of phosphatidylcholine increases significantly by application of vacuum, even though a slight decrease of n-3 polyunsaturated fatty acid incorporation is observed Fusarium oxysporum
3.1.1.32 additional information enzyme immobilization using Lewatit VP OC 1600 as immobilization carrier, method, n-3 polyunsaturated fatty acids-enriched phosphatidylcholine is successfully produced with fatty acid from fish oil and phosphatidylcholine from soybean by immobilized phospholipase A1-catalyzed acidolysis, overview. The yield of phosphatidylcholine increases significantly by application of vacuum, even though a slight decrease of n-3 polyunsaturated fatty acid incorporation is observed Thermomyces lanuginosus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.32 Fusarium oxysporum
-
-
-
3.1.1.32 Thermomyces lanuginosus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.32 phosphatidylcholine + H2O substrate from egg yolk Fusarium oxysporum 2-acylglycerophosphocholine + a carboxylate
-
 ?
3.1.1.32 phosphatidylcholine + H2O substrate from egg yolk Thermomyces lanuginosus 2-acylglycerophosphocholine + a carboxylate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.32 PLA1
-
 Fusarium oxysporum
3.1.1.32 PLA1
-
 Thermomyces lanuginosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.32 55
-
 immobilized enzyme Fusarium oxysporum
3.1.1.32 55
-
 immobilized enzyme Thermomyces lanuginosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.32 35 65 activity range, immobilized enzyme Fusarium oxysporum
3.1.1.32 35 65 activity range, immobilized enzyme Thermomyces lanuginosus