BRENDA - Enzyme Database

Ornithine cyclodeaminase/mu-crystallin homolog from the hyperthermophilic archaeon Thermococcus litoralis functions as a novel DELTA(1)-pyrroline-2-carboxylate reductase involved in putative trans-3-hydroxy-L-proline metabolism

Watanabe, S.; Tozawa, Y.; Watanabe, Y.; FEBS Open Bio 4, 617-626 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.5.1.B5
His-tagged protein is expressed in Escherichia coli BL21. TlLhpI is a fusion of OCC_00362 and OCC_00367 genes by PCR. The gene from Thermococcus litoralis DSM 5473 consists of two separate open-reading frames (ORFs): OCC_00362 and OCC_00367. When a single A is inserted between A227646 and T227647 in the original genome sequence of T. litoralis DSM 5473, the separate ORFs fuse into a single ORF, and the putative amino acid sequence of is homologous to that of the Thermococcus sibiricus TSIB_0634 gene. Although there is a possibility that this OCD-like protein from Thermococcus litoralis DSM 5473 forms a heterooligomeric structure, the TlLhpI gene is synthesized as a single ORF by sequential steps of PCR using sense and antisense primers and genome DNA of Thermococcus litoralis DSM 5473 as a template
Thermococcus litoralis
4.2.1.77
-
Thermococcus litoralis
Engineering
EC Number
Protein Variants
Commentary
Organism
1.5.1.B5
L52E/V224D/A228K
DELTA1-pyrroline-2-carboxylate reductase activity is the same as in wild-type enzyme, no alanine dehydrogenase activity
Thermococcus litoralis
1.5.1.B5
L52R/V224D/A228K
DELTA1-pyrroline-2-carboxylate reductase activity is the same as in wild-type enzyme, no alanine dehydrogenase activity
Thermococcus litoralis
1.5.1.B5
additional information
TlLhpI is a fusion of OCC_00362 and OCC_00367 genes by PCR. The gene from Thermococcus litoralis DSM 5473 consistsof two separate open-reading frames (ORFs): OCC_00362 and OCC_00367. When a single A is inserted between A227646 and T227647 in the original genome sequence of T. litoralis DSM 5473, the separate ORFs fuse into a single ORF, and the putative amino acid sequence of is homologous to that of the Thermococcus sibiricus TSIB_0634 gene. Although there is a possibility that this OCD-like protein from Thermococcus litoralis DSM 5473 forms a heterooligomeric structure, the TlLhpI gene is synthesized as a single ORF by sequential steps of PCR using sense and antisense primers and genome DNA of Thermococcus litoralis DSM 5473 as a template
Thermococcus litoralis
1.5.1.B5
V224D/A228K
12fold enhancement of DELTA1-pyrroline-2-carboxylate reductase activity, 6.7fold enhancement for the oxidization activity for L-proline, no alanine dehydrogenase activity
Thermococcus litoralis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.5.1.B5
0.444
-
L-proline
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
1.5.1.B5
0.608
-
L-pipecolate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.5.1.B5
0.944
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.5.1.B5
1.12
-
L-proline
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.5.1.B5
2.77
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.5.1.B5
40000
-
2 * 40000, SDS-PAGE
Thermococcus litoralis
1.5.1.B5
72000
-
gel filtration
Thermococcus litoralis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.5.1.B5
DELTA1-pyrroline-2-carboxylate + NADH + H+
Thermococcus litoralis
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
L-proline + NAD+
-
-
?
1.5.1.B5
DELTA1-pyrroline-2-carboxylate + NADH + H+
Thermococcus litoralis DSM 5473
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
L-proline + NAD+
-
-
?
4.2.1.77
trans-3-hydroxy-L-proline
Thermococcus litoralis
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
1-pyrroline 2-carboxylate + H2O
-
-
?
4.2.1.77
trans-3-hydroxy-L-proline
Thermococcus litoralis DSM 5473
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
1-pyrroline 2-carboxylate + H2O
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.5.1.B5
Thermococcus litoralis
H3ZMH3 and H3ZMH4
TlLhpI is a fusion of OCC_00362 and OCC_00367 genes by PCR. The gene from Thermococcus litoralis DSM 5473 consists of two separate open-reading frames (ORFs): OCC_00362 and OCC_00367. When a single A is inserted between A227646 and T227647 in the original genome sequence of T. litoralis DSM 5473, the separate ORFs fuse into a single ORF, and the putative amino acid sequence of is homologous to that of the Thermococcus sibiricus TSIB_0634 gene. Although there is a possibility that this OCD-like protein from Thermococcus litoralis DSM 5473 forms a heterooligomeric structure, the TlLhpI gene is synthesized as a single ORF by sequential steps of PCR using sense and antisense primers and genome DNA of Thermococcus litoralis DSM 5473 as a template
-
1.5.1.B5
Thermococcus litoralis DSM 5473
H3ZMH3 and H3ZMH4
TlLhpI is a fusion of OCC_00362 and OCC_00367 genes by PCR. The gene from Thermococcus litoralis DSM 5473 consists of two separate open-reading frames (ORFs): OCC_00362 and OCC_00367. When a single A is inserted between A227646 and T227647 in the original genome sequence of T. litoralis DSM 5473, the separate ORFs fuse into a single ORF, and the putative amino acid sequence of is homologous to that of the Thermococcus sibiricus TSIB_0634 gene. Although there is a possibility that this OCD-like protein from Thermococcus litoralis DSM 5473 forms a heterooligomeric structure, the TlLhpI gene is synthesized as a single ORF by sequential steps of PCR using sense and antisense primers and genome DNA of Thermococcus litoralis DSM 5473 as a template
-
4.2.1.77
Thermococcus litoralis
H3ZMH8
-
-
4.2.1.77
Thermococcus litoralis DSM 5473
H3ZMH8
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.5.1.B5
-
Thermococcus litoralis
4.2.1.77
-
Thermococcus litoralis
Reaction
EC Number
Reaction
Commentary
Organism
Reaction ID
1.5.1.B5
L-pipecolate + NAD+ = DELTA1-piperideine-2-carboxylate + NADH + H+
(1)
Thermococcus litoralis
1.5.1.B5
L-proline + NAD+ = DELTA1-pyrroline-2-carboxylate + NADH + H+
(2)
Thermococcus litoralis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.5.1.B5
cis-4-hydroxy-L-proline + NAD+
activity is 85% compared to the activity with L-proline
729750
Thermococcus litoralis
?
-
-
-
?
1.5.1.B5
cis-4-hydroxy-L-proline + NAD+
activity is 85% compared to the activity with L-proline
729750
Thermococcus litoralis DSM 5473
?
-
-
-
?
1.5.1.B5
DELTA1-pyrroline-2-carboxylate + NADH + H+
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
729750
Thermococcus litoralis
L-proline + NAD+
-
-
-
?
1.5.1.B5
DELTA1-pyrroline-2-carboxylate + NADH + H+
preference of the reaction equilibrium in the direction toward NADH-dependent reduction
729750
Thermococcus litoralis
L-proline + NAD+
-
-
-
r
1.5.1.B5
DELTA1-pyrroline-2-carboxylate + NADH + H+
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
729750
Thermococcus litoralis DSM 5473
L-proline + NAD+
-
-
-
?
1.5.1.B5
DELTA1-pyrroline-2-carboxylate + NADH + H+
preference of the reaction equilibrium in the direction toward NADH-dependent reduction
729750
Thermococcus litoralis DSM 5473
L-proline + NAD+
-
-
-
r
1.5.1.B5
L-pipecolate + NAD+
activity is 49% compared to the activity with L-proline
729750
Thermococcus litoralis
DELTA1-piperideine-2-carboxylate + NADH + H+
-
-
-
?
1.5.1.B5
L-pipecolate + NAD+
activity is 49% compared to the activity with L-proline
729750
Thermococcus litoralis DSM 5473
DELTA1-piperideine-2-carboxylate + NADH + H+
-
-
-
?
1.5.1.B5
L-proline + NAD+
preference of the reaction equilibrium in the direction toward NADH-dependent reduction
729750
Thermococcus litoralis
DELTA1-pyrroline-2-carboxylate + NADH + H+
-
-
-
r
1.5.1.B5
additional information
the enzyme also shows alanine dehydrogenase activity (EC 1.4.1.1) and N-methyl-L-alanine dehydrogenase activity (EC 1.4.1.17)
729750
Thermococcus litoralis
?
-
-
-
-
1.5.1.B5
additional information
the enzyme also shows alanine dehydrogenase activity (EC 1.4.1.1) and N-methyl-L-alanine dehydrogenase activity (EC 1.4.1.17)
729750
Thermococcus litoralis DSM 5473
?
-
-
-
-
1.5.1.B5
trans-4-hydroxy-L-proline + NAD+
activity is 70% compared to the activity with L-proline
729750
Thermococcus litoralis
?
-
-
-
?
4.2.1.77
trans-3-hydroxy-L-proline
-
729750
Thermococcus litoralis
1-pyrroline 2-carboxylate + H2O
-
-
-
?
4.2.1.77
trans-3-hydroxy-L-proline
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
729750
Thermococcus litoralis
1-pyrroline 2-carboxylate + H2O
-
-
-
?
4.2.1.77
trans-3-hydroxy-L-proline
-
729750
Thermococcus litoralis DSM 5473
1-pyrroline 2-carboxylate + H2O
-
-
-
?
4.2.1.77
trans-3-hydroxy-L-proline
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
729750
Thermococcus litoralis DSM 5473
1-pyrroline 2-carboxylate + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.5.1.B5
dimer
2 * 40000, SDS-PAGE
Thermococcus litoralis
Synonyms
EC Number
Synonyms
Commentary
Organism
1.5.1.B5
DELTA1-pyrroline-2-carboxylate reductase
misleading
Thermococcus litoralis
1.5.1.B5
Pyr2C reductase
-
Thermococcus litoralis
1.5.1.B5
TlLhpI
-
Thermococcus litoralis
4.2.1.77
T3LHyp dehydratase
-
Thermococcus litoralis
4.2.1.77
TlLhpH
-
Thermococcus litoralis
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.5.1.B5
50
-
assay at
Thermococcus litoralis
4.2.1.77
80
100
-
Thermococcus litoralis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.5.1.B5
0.2
-
L-pipecolate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.5.1.B5
0.23
-
L-proline
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.5.1.B5
0.61
-
L-proline
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
1.5.1.B5
16.6
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.5.1.B5
558
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.5.1.B5
4
6
substrates: DELTA1-pyrroline-2-carboxylate + NADH + H+
Thermococcus litoralis
1.5.1.B5
11
-
substrates: L-proline + NAD+
Thermococcus litoralis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.5.1.B5
5
11
pH 5.0: about 40% of maximal activity, pH 11.0: about 80% of maximal activity
Thermococcus litoralis
1.5.1.B5
10
12
pH 10.0: about 75% of maximal activity, pH 12.0: about 85% of maximal activity
Thermococcus litoralis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.5.1.B5
NAD+
the kcat/Km value for proline in the presence of NADP+ is about 50fold lower than that in the presence of NAD+
Thermococcus litoralis
1.5.1.B5
NADH
no activity with NADPH
Thermococcus litoralis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.5.1.B5
His-tagged protein is expressed in Escherichia coli BL21. TlLhpI is a fusion of OCC_00362 and OCC_00367 genes by PCR. The gene from Thermococcus litoralis DSM 5473 consists of two separate open-reading frames (ORFs): OCC_00362 and OCC_00367. When a single A is inserted between A227646 and T227647 in the original genome sequence of T. litoralis DSM 5473, the separate ORFs fuse into a single ORF, and the putative amino acid sequence of is homologous to that of the Thermococcus sibiricus TSIB_0634 gene. Although there is a possibility that this OCD-like protein from Thermococcus litoralis DSM 5473 forms a heterooligomeric structure, the TlLhpI gene is synthesized as a single ORF by sequential steps of PCR using sense and antisense primers and genome DNA of Thermococcus litoralis DSM 5473 as a template
Thermococcus litoralis
4.2.1.77
-
Thermococcus litoralis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.5.1.B5
NAD+
the kcat/Km value for proline in the presence of NADP+ is about 50fold lower than that in the presence of NAD+
Thermococcus litoralis
1.5.1.B5
NADH
no activity with NADPH
Thermococcus litoralis
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
1.5.1.B5
L52E/V224D/A228K
DELTA1-pyrroline-2-carboxylate reductase activity is the same as in wild-type enzyme, no alanine dehydrogenase activity
Thermococcus litoralis
1.5.1.B5
L52R/V224D/A228K
DELTA1-pyrroline-2-carboxylate reductase activity is the same as in wild-type enzyme, no alanine dehydrogenase activity
Thermococcus litoralis
1.5.1.B5
additional information
TlLhpI is a fusion of OCC_00362 and OCC_00367 genes by PCR. The gene from Thermococcus litoralis DSM 5473 consistsof two separate open-reading frames (ORFs): OCC_00362 and OCC_00367. When a single A is inserted between A227646 and T227647 in the original genome sequence of T. litoralis DSM 5473, the separate ORFs fuse into a single ORF, and the putative amino acid sequence of is homologous to that of the Thermococcus sibiricus TSIB_0634 gene. Although there is a possibility that this OCD-like protein from Thermococcus litoralis DSM 5473 forms a heterooligomeric structure, the TlLhpI gene is synthesized as a single ORF by sequential steps of PCR using sense and antisense primers and genome DNA of Thermococcus litoralis DSM 5473 as a template
Thermococcus litoralis
1.5.1.B5
V224D/A228K
12fold enhancement of DELTA1-pyrroline-2-carboxylate reductase activity, 6.7fold enhancement for the oxidization activity for L-proline, no alanine dehydrogenase activity
Thermococcus litoralis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.5.1.B5
0.444
-
L-proline
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
1.5.1.B5
0.608
-
L-pipecolate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.5.1.B5
0.944
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.5.1.B5
1.12
-
L-proline
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.5.1.B5
2.77
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.5.1.B5
40000
-
2 * 40000, SDS-PAGE
Thermococcus litoralis
1.5.1.B5
72000
-
gel filtration
Thermococcus litoralis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.5.1.B5
DELTA1-pyrroline-2-carboxylate + NADH + H+
Thermococcus litoralis
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
L-proline + NAD+
-
-
?
1.5.1.B5
DELTA1-pyrroline-2-carboxylate + NADH + H+
Thermococcus litoralis DSM 5473
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
L-proline + NAD+
-
-
?
4.2.1.77
trans-3-hydroxy-L-proline
Thermococcus litoralis
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
1-pyrroline 2-carboxylate + H2O
-
-
?
4.2.1.77
trans-3-hydroxy-L-proline
Thermococcus litoralis DSM 5473
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
1-pyrroline 2-carboxylate + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.5.1.B5
-
Thermococcus litoralis
4.2.1.77
-
Thermococcus litoralis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.5.1.B5
cis-4-hydroxy-L-proline + NAD+
activity is 85% compared to the activity with L-proline
729750
Thermococcus litoralis
?
-
-
-
?
1.5.1.B5
cis-4-hydroxy-L-proline + NAD+
activity is 85% compared to the activity with L-proline
729750
Thermococcus litoralis DSM 5473
?
-
-
-
?
1.5.1.B5
DELTA1-pyrroline-2-carboxylate + NADH + H+
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
729750
Thermococcus litoralis
L-proline + NAD+
-
-
-
?
1.5.1.B5
DELTA1-pyrroline-2-carboxylate + NADH + H+
preference of the reaction equilibrium in the direction toward NADH-dependent reduction
729750
Thermococcus litoralis
L-proline + NAD+
-
-
-
r
1.5.1.B5
DELTA1-pyrroline-2-carboxylate + NADH + H+
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
729750
Thermococcus litoralis DSM 5473
L-proline + NAD+
-
-
-
?
1.5.1.B5
DELTA1-pyrroline-2-carboxylate + NADH + H+
preference of the reaction equilibrium in the direction toward NADH-dependent reduction
729750
Thermococcus litoralis DSM 5473
L-proline + NAD+
-
-
-
r
1.5.1.B5
L-pipecolate + NAD+
activity is 49% compared to the activity with L-proline
729750
Thermococcus litoralis
DELTA1-piperideine-2-carboxylate + NADH + H+
-
-
-
?
1.5.1.B5
L-pipecolate + NAD+
activity is 49% compared to the activity with L-proline
729750
Thermococcus litoralis DSM 5473
DELTA1-piperideine-2-carboxylate + NADH + H+
-
-
-
?
1.5.1.B5
L-proline + NAD+
preference of the reaction equilibrium in the direction toward NADH-dependent reduction
729750
Thermococcus litoralis
DELTA1-pyrroline-2-carboxylate + NADH + H+
-
-
-
r
1.5.1.B5
additional information
the enzyme also shows alanine dehydrogenase activity (EC 1.4.1.1) and N-methyl-L-alanine dehydrogenase activity (EC 1.4.1.17)
729750
Thermococcus litoralis
?
-
-
-
-
1.5.1.B5
additional information
the enzyme also shows alanine dehydrogenase activity (EC 1.4.1.1) and N-methyl-L-alanine dehydrogenase activity (EC 1.4.1.17)
729750
Thermococcus litoralis DSM 5473
?
-
-
-
-
1.5.1.B5
trans-4-hydroxy-L-proline + NAD+
activity is 70% compared to the activity with L-proline
729750
Thermococcus litoralis
?
-
-
-
?
4.2.1.77
trans-3-hydroxy-L-proline
-
729750
Thermococcus litoralis
1-pyrroline 2-carboxylate + H2O
-
-
-
?
4.2.1.77
trans-3-hydroxy-L-proline
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
729750
Thermococcus litoralis
1-pyrroline 2-carboxylate + H2O
-
-
-
?
4.2.1.77
trans-3-hydroxy-L-proline
-
729750
Thermococcus litoralis DSM 5473
1-pyrroline 2-carboxylate + H2O
-
-
-
?
4.2.1.77
trans-3-hydroxy-L-proline
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
729750
Thermococcus litoralis DSM 5473
1-pyrroline 2-carboxylate + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.5.1.B5
dimer
2 * 40000, SDS-PAGE
Thermococcus litoralis
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.5.1.B5
50
-
assay at
Thermococcus litoralis
4.2.1.77
80
100
-
Thermococcus litoralis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.5.1.B5
0.2
-
L-pipecolate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.5.1.B5
0.23
-
L-proline
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.5.1.B5
0.61
-
L-proline
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
1.5.1.B5
16.6
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.5.1.B5
558
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.5.1.B5
4
6
substrates: DELTA1-pyrroline-2-carboxylate + NADH + H+
Thermococcus litoralis
1.5.1.B5
11
-
substrates: L-proline + NAD+
Thermococcus litoralis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.5.1.B5
5
11
pH 5.0: about 40% of maximal activity, pH 11.0: about 80% of maximal activity
Thermococcus litoralis
1.5.1.B5
10
12
pH 10.0: about 75% of maximal activity, pH 12.0: about 85% of maximal activity
Thermococcus litoralis
General Information
EC Number
General Information
Commentary
Organism
1.5.1.B5
metabolism
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
Thermococcus litoralis
4.2.1.77
metabolism
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
Thermococcus litoralis
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.5.1.B5
metabolism
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
Thermococcus litoralis
4.2.1.77
metabolism
the enzyme is involved in the trans-3-hydroxy-L-proline metabolism
Thermococcus litoralis
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.5.1.B5
0.2
-
L-proline
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.5.1.B5
0.33
-
L-pipecolate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.5.1.B5
1.4
-
L-proline
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
1.5.1.B5
17.6
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.5.1.B5
201
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.5.1.B5
0.2
-
L-proline
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.5.1.B5
0.33
-
L-pipecolate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.5.1.B5
1.4
-
L-proline
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis
1.5.1.B5
17.6
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, wild-type enzyme
Thermococcus litoralis
1.5.1.B5
201
-
DELTA1-pyrroline-2-carboxylate
pH 6.0, 50°C, mutant enzyme V224D/A228K
Thermococcus litoralis