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Literature summary extracted from

  • Watanabe, S.; Tanimoto, Y.; Yamauchi, S.; Tozawa, Y.; Sawayama, S.; Watanabe, Y.
    Identification and characterization of trans-3-hydroxy-L-proline dehydratase and DELTA(1)-pyrroline-2-carboxylate reductase involved in trans-3-hydroxy-L-proline metabolism of bacteria (2014), FEBS Open Bio, 4, 240-250.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.49 2.79
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, cosubstrate: NADH Colwellia psychrerythraea
1.5.1.49 5.9
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, cosubstrate: NADPH Colwellia psychrerythraea
1.5.1.49 18.3
-
 L-proline pH 10.5, 30°C, cosubstrate: NADP+ Colwellia psychrerythraea
1.5.1.49 18.8
-
 L-proline pH 10.5, 30°C, cosubstrate: NAD+ Colwellia psychrerythraea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.49 DELTA1-pyrroline-2-carboxylate + NADH + H+ Colwellia psychrerythraea the enzyme is part of the trans-3-hydroxy-L-proline pathway L-proline + NAD+
-
 ?
1.5.1.49 DELTA1-pyrroline-2-carboxylate + NADPH + H+ Colwellia psychrerythraea the enzyme is part of the trans-3-hydroxy-L-proline pathway L-proline + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.49 Colwellia psychrerythraea Q485S0
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.49 0.00451
-
 L-proline, pH 10.5, 30°C, cosubstrate: NAD+ Colwellia psychrerythraea
1.5.1.49 0.63
-
 L-proline, pH 10.5, 30°C, cosubstrate: NADP+ Colwellia psychrerythraea
1.5.1.49 4.76
-
 DELTA1-pyrroline-2-carboxylate + NADH, pH 6.5, 30°C Colwellia psychrerythraea
1.5.1.49 30.2
-
 DELTA1-pyrroline-2-carboxylate + NADPH, pH 6.5, 30°C Colwellia psychrerythraea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.49 DELTA1-pyrroline-2-carboxylate + NADH + H+ the enzyme is part of the trans-3-hydroxy-L-proline pathway Colwellia psychrerythraea L-proline + NAD+
-
 ?
1.5.1.49 DELTA1-pyrroline-2-carboxylate + NADH + H+ preference of the reaction equilibrium to the direction toward NADPH dependent reduction. The kcat/Km value for DELTA1-pyrroline-2-carboxylate in the presence of NADH is 5.6fold lower than that in the presence of NADPH, mainly caused by the 12fold lower kcat value Colwellia psychrerythraea L-proline + NAD+
-
 ?
1.5.1.49 DELTA1-pyrroline-2-carboxylate + NADPH + H+ the enzyme is part of the trans-3-hydroxy-L-proline pathway Colwellia psychrerythraea L-proline + NADP+
-
 ?
1.5.1.49 DELTA1-pyrroline-2-carboxylate + NADPH + H+ preference of the reaction equilibrium to the direction toward NADPH dependent reduction. The preferred cofactor is NADPH, lower activity with NADH. The kcat/Km value for DELTA1-pyrroline-2-carboxylate in the presence of NADH is 5.6fold lower than that in the presence of NADPH, mainly caused by the 12fold lower kcat value Colwellia psychrerythraea L-proline + NADP+
-
 ?
1.5.1.49 L-proline + NAD+ preference of the reaction equilibrium to the direction toward NADH dependent reduction. In the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1 Colwellia psychrerythraea DELTA1-pyrroline-2-carboxylate + NADH + H+
-
 r
1.5.1.49 L-proline + NADP+ preference of the reaction equilibrium to the direction toward NADH dependent reduction. In the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1 Colwellia psychrerythraea DELTA1-pyrroline-2-carboxylate + NADPH + H+
-
 r
1.5.1.49 additional information no significant activity with DELTA1-piperideine-2-carboxylate or L-pipecolate. The kcat/Km values for DELTA1-piperideine-2-carboxylate and L-pipecolate in the presence of NADP+(H) are 111fold and 223fold lower than those for DELTA1-pyrroline-2-carboxylate and L-proline, mainly caused by 90fold and 51fold lower kcat values, respectively Colwellia psychrerythraea ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.49 LhpH
-
 Colwellia psychrerythraea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.49 30
-
 assay at Colwellia psychrerythraea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.49 0.0069
-
 L-proline pH 10.5, 30°C, cosubstrate: NAD+ Colwellia psychrerythraea
1.5.1.49 1.01
-
 L-proline pH 10.5, 30°C, cosubstrate: NADP+ Colwellia psychrerythraea
1.5.1.49 10.35
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, cosubstrate: NADH Colwellia psychrerythraea
1.5.1.49 124.5
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, cosubstrate: NADPH Colwellia psychrerythraea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.49 6.5
-
 assay at, reduction of DELTA1-pyrroline-2-carboxylate Colwellia psychrerythraea
1.5.1.49 10.5
-
 assay at, oxidation of L-proline Colwellia psychrerythraea

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.49 NAD+ in the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1 Colwellia psychrerythraea
1.5.1.49 NADH the preferred cofactor is NADPH, lower activity with NADH. The kcat/Km value for DELTA1-pyrroline-2-carboxylate in the presence of NADH is 5.6fold lower than that in the presence of NADPH, mainly caused by the 12fold lower kcat value Colwellia psychrerythraea
1.5.1.49 NADP+ in the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1 Colwellia psychrerythraea
1.5.1.49 NADPH the preferred cofactor is NADPH, lower activity with NADH. The kcat/Km value for DELTA1-pyrroline-2-carboxylate in the presence of NADH is 5.6fold lower than that in the presence of NADPH, mainly caused by the 12fold lower kcat value Colwellia psychrerythraea

General Information

EC Number General Information Comment Organism
1.5.1.49 metabolism the enzyme is part of the trans-3-hydroxy-L-proline pathway Colwellia psychrerythraea

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.1.49 0.000367
-
 L-proline pH 10.5, 30°C, cosubstrate: NAD+ Colwellia psychrerythraea
1.5.1.49 0.05519
-
 L-proline pH 10.5, 30°C, cosubstrate: NADP+ Colwellia psychrerythraea
1.5.1.49 3.7
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, cosubstrate: NADH Colwellia psychrerythraea
1.5.1.49 21.1
-
 DELTA1-pyrroline-2-carboxylate pH 6.5, 30°C, cosubstrate: NADPH Colwellia psychrerythraea