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Literature summary extracted from

  • Zhang, Y.; Stevens, J.M.; Ferguson, S.J.
    Substrate recognition of holocytochrome c synthase: N-terminal region and CXXCH motif of mitochondrial cytochrome c (2014), FEBS Lett., 588, 3367-3374.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.17 coexpression of the wild-type enzyme with periplasmic targeting sequence and addition of the Pseudomonas aeruginosa cytochrome c551/552 pre-sequence, and mutant enzymes with Equus caballus heart cytochrome c or Strep-tagged Saccharomyces cerevisiaecytochrome c in Escherichia coli strain BL21(DE3) Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
4.4.1.17 C15A site-directed mutagenesis, cytoplasm-targeted mutant, the mutant is matured and undergoes heme attachment like the wild-type enzyme Saccharomyces cerevisiae
4.4.1.17 C18A site-directed mutagenesis, cytoplasm-targeted mutant, the mutant does not undergo heme attachment Saccharomyces cerevisiae
4.4.1.17 F15A site-directed mutagenesis, periplasm-targeted mutant, the mutant variant does not produce holocytochrome c Saccharomyces cerevisiae
4.4.1.17 F15E site-directed mutagenesis, periplasm-targeted mutant, the mutant variant does not produce holocytochrome c Saccharomyces cerevisiae
4.4.1.17 F15I site-directed mutagenesis, periplasm-targeted mutant, the mutant shows reduced holocytochrome c production compared to the wild-type enzyme Saccharomyces cerevisiae
4.4.1.17 F15W site-directed mutagenesis, periplasm-targeted mutant, the mutant shows reduced holocytochrome c production compared to the wild-type enzyme Saccharomyces cerevisiae
4.4.1.17 F15Y site-directed mutagenesis, periplasm-targeted mutant, the mutant shows unaltered holocytochrome c production like the wild-type enzyme Saccharomyces cerevisiae
4.4.1.17 H19K site-directed mutagenesis, cytoplasm-targeted mutant, the mutant does not undergo heme attachment Saccharomyces cerevisiae
4.4.1.17 H19M site-directed mutagenesis, cytoplasm-targeted mutant, the mutant does not undergo heme attachment Saccharomyces cerevisiae
4.4.1.17 H19R site-directed mutagenesis, cytoplasm-targeted mutant, the mutant does not undergo heme attachment Saccharomyces cerevisiae
4.4.1.17 additional information construction of T17, K16, G29, H45, and K60 deletion mutants and of a mutant with periplasmic targeting sequence Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.4.1.17 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.17 Apocytochrome c + heme Saccharomyces cerevisiae
-
 Holocytochrome c
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.17 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.17 Apocytochrome c + heme
-
 Saccharomyces cerevisiae Holocytochrome c
-
 ?
4.4.1.17 Apocytochrome c + heme Equus caballus heart cytochrome c or Strep-tagged Saccharomyces cerevisiae cytochrome c are coexpressed in Escherichia coli strain BL21(DE3) with Saccharomyces cerevisiae holocytochrome c synthase. No product synthesis from truncated cytochrome c1 mutants G29X, H45X and K60X, with the X indicating the position of the inserted stop codon Saccharomyces cerevisiae Holocytochrome c
-
 ?
4.4.1.17 additional information the CXXCH motif and the N-terminus of the apocytochrome polypeptide are important protein-protein recognition motifs in enzyme-substrate interaction, the N-terminal region of the mitochondrial cytochrome c sequence is critical for attachment of heme to apocytochrome c Saccharomyces cerevisiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.17 HCC synthase
-
 Saccharomyces cerevisiae
4.4.1.17 HCCS
-
 Saccharomyces cerevisiae
4.4.1.17 holocytochrome c synthase
-
 Saccharomyces cerevisiae