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Literature summary extracted from

  • Moore, R.L.; Stevens, J.M.; Ferguson, S.J.
    Mitochondrial cytochrome c synthase: CP motifs are not necessary for heme attachment to apocytochrome c (2011), FEBS Lett., 585, 3415-3419.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.17 recombinant expression of His-tagged wild-type enzyme and mutants in Escherichia coli strain BL21(DE3) cytoplasm, coexpression with Equus caballus cytochrome c Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
4.4.1.17 C26A site-directed mutagenesis of CP-motif 1 of the enzyme, the mutant shows activity similar to the wild-type enzyme Saccharomyces cerevisiae
4.4.1.17 C26A/C42A site-directed mutagenesis of both CP-motifs of the enzyme, the mutant shows activity similar to the wild-type enzyme Saccharomyces cerevisiae
4.4.1.17 C26A/P27A site-directed mutagenesis of CP-motif 1 of the enzyme, the mutant shows activity similar to the wild-type enzyme Saccharomyces cerevisiae
4.4.1.17 C42A site-directed mutagenesis of CP-motif 2 of the enzyme, the mutant shows activity similar to the wild-type enzyme Saccharomyces cerevisiae
4.4.1.17 C42A/P43A site-directed mutagenesis of CP-motif 2 of the enzyme, the mutant shows activity similar to the wild-type enzyme Saccharomyces cerevisiae
4.4.1.17 E133A site-directed mutagenesis, the mutant shows highly reduced activity compared with the wild-type enzyme Saccharomyces cerevisiae
4.4.1.17 E133K site-directed mutagenesis, the mutant shows highly reduced activity compared with the wild-type enzyme Saccharomyces cerevisiae
4.4.1.17 R199A site-directed mutagenesis, almost inactive mutant Saccharomyces cerevisiae
4.4.1.17 R199C site-directed mutagenesis, almost inactive mutant Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.17 Apocytochrome c + heme Saccharomyces cerevisiae
-
 Holocytochrome c
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.17 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.17 Apocytochrome c + heme
-
 Saccharomyces cerevisiae Holocytochrome c
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.17 HCCS
-
 Saccharomyces cerevisiae
4.4.1.17 heme lyase
-
 Saccharomyces cerevisiae
4.4.1.17 holocytochrome c synthase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.17 37
-
 assay at Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
4.4.1.17 additional information the CP motifs, commonly found in heme-binding proteins, are not required for enzyme activity of te Saccharomyyces cerevisiae enzyme, while mutations in the human enzyme on the C-terminal side of the CP motifs cause disease states of microphthalmia with linear skin defects due to abolished or drastically attenuated holocytochrome c production Saccharomyces cerevisiae