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Literature summary extracted from

  • Tanaka, T.; Kida, T.; Imai, H.; Morishige, J.; Yamashita, R.; Matsuoka, H.; Uozumi, S.; Satouchi, K.; Nagano, M.; Tokumura, A.
    Identification of a sphingolipid-specific phospholipase D activity associated with the generation of phytoceramide-1-phosphate in cabbage leaves (2013), FEBS J., 280, 3797-3809.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.4 EDTA complete inhibition Brassica oleracea
3.1.4.4 additional information no inhibiton by EDTA Brassica oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.4 membrane
-
 Brassica oleracea 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.4 Ca2+ absolutely dependent on, no activity without Ca2+ Brassica oleracea
3.1.4.4 Ca2+ the enzyme is activated by Ca2+, in absence of Ca2+ it exhibits 70% of maximal activity Brassica oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.4 phosphatidylcholine + H2O Brassica oleracea
-
 choline + phosphatidate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Arabidopsis thaliana
-
-
-
3.1.4.4 Brassica oleracea
-
-
-
3.1.4.4 Capsella bursa-pastoris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.4 native enzyme partially by preparation of the 13000 rpm fraction Arabidopsis thaliana
3.1.4.4 native enzyme partially by preparation of the 13000 rpm fraction Capsella bursa-pastoris
3.1.4.4 native enzyme partially by preparation of the membrane fraction, solubilization with Triton X-100, ammonium sulfate fractionation, and diethylaminoethyl-cellulose chromatography Brassica oleracea
3.1.4.4 native enzyme partially from cabbage leaves by acetone precipitation Brassica oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.4 leaf
-
 Arabidopsis thaliana
-
3.1.4.4 leaf
-
 Brassica oleracea
-
3.1.4.4 leaf
-
 Capsella bursa-pastoris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.4 0.0005
-
 partially purified enzyme, pH 7.4, 30°C Capsella bursa-pastoris
3.1.4.4 0.0011
-
 partially purified enzyme, pH 7.4, 30°C Arabidopsis thaliana
3.1.4.4 0.03 0.04 partially purifed enzyme, pH 7.4, 30°C Brassica oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 glycosylinositol phosphoceramide + H2O
-
 Arabidopsis thaliana phytoceramide-1-phosphate + glycosylinositol phytoceramide-1-phosphate with an alpha-hydroxy fatty acid ?
3.1.4.4 glycosylinositol phosphoceramide + H2O
-
 Brassica oleracea phytoceramide-1-phosphate + glycosylinositol phytoceramide-1-phosphate with an alpha-hydroxy fatty acid ?
3.1.4.4 glycosylinositol phosphoceramide + H2O
-
 Capsella bursa-pastoris phytoceramide-1-phosphate + glycosylinositol phytoceramide-1-phosphate with an alpha-hydroxy fatty acid ?
3.1.4.4 additional information the enzyme does not hydrolyze glycosylinositol phosphoceramide Brassica oleracea ?
-
 ?
3.1.4.4 additional information the enzyme hydrolyzes glycosylinositol phosphoceramide specifically, but not glycerophospholipids, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, or sphingomyelin, MALDI-TOF mass spectrometry product identification, overview Arabidopsis thaliana ?
-
 ?
3.1.4.4 additional information the enzyme hydrolyzes glycosylinositol phosphoceramide specifically, but not glycerophospholipids, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, or sphingomyelin, MALDI-TOF mass spectrometry product identification, overview Brassica oleracea ?
-
 ?
3.1.4.4 additional information the enzyme hydrolyzes glycosylinositol phosphoceramide specifically, but not glycerophospholipids, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, or sphingomyelin, MALDI-TOF mass spectrometry product identification, overview Capsella bursa-pastoris ?
-
 ?
3.1.4.4 phosphatidylcholine + H2O
-
 Brassica oleracea choline + phosphatidate
-
 ?
3.1.4.4 phosphatidylcholine + H2O from egg yolk Brassica oleracea choline + phosphatidate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.4 GIPC-PLD
-
 Arabidopsis thaliana
3.1.4.4 GIPC-PLD
-
 Brassica oleracea
3.1.4.4 GIPC-PLD
-
 Capsella bursa-pastoris
3.1.4.4 PC-PLD
-
 Brassica oleracea
3.1.4.4 PLD
-
 Arabidopsis thaliana
3.1.4.4 PLD
-
 Brassica oleracea
3.1.4.4 PLD
-
 Capsella bursa-pastoris
3.1.4.4 sphingolipid-specific phospholipase D
-
 Arabidopsis thaliana
3.1.4.4 sphingolipid-specific phospholipase D
-
 Brassica oleracea
3.1.4.4 sphingolipid-specific phospholipase D
-
 Capsella bursa-pastoris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.4 30
-
 assay at Arabidopsis thaliana
3.1.4.4 30
-
 assay at Brassica oleracea
3.1.4.4 30
-
 assay at Capsella bursa-pastoris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.4 5.5
-
-
 Brassica oleracea
3.1.4.4 7.4
-
 assay at Arabidopsis thaliana
3.1.4.4 7.4
-
 assay at Capsella bursa-pastoris
3.1.4.4 7.5
-
-
 Brassica oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.4 4.5 8.5 activity range Brassica oleracea
3.1.4.4 4.5 7 activity range Brassica oleracea