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Literature summary extracted from
- Purification and characterisation of thermo-alkaline pectinase enzyme from Hylocereus polyrhizus (2014), Eur. Food Res. Technol., 239, 21-29.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.2.10 | L-cystine | 5 mM, 142% of initial activity | Hylocereus polyrhizus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.2.10 | Al3+ | 10 mM, 5% residual activity | Hylocereus polyrhizus | |
| 4.2.2.10 | Fe2+ | 10 mM, no residual activity | Hylocereus polyrhizus | |
| 4.2.2.10 | N-bromosuccinimide | 5 mM, no residual activity | Hylocereus polyrhizus | |
| 4.2.2.10 | Ni2+ | 10 mM, 24% residual activity | Hylocereus polyrhizus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.2.10 | additional information | - |
polygalacturonic acid | Km value 2.7 mg/ml, pH 8.0, 75°C | Hylocereus polyrhizus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.2.10 | Ca2+ | 10 mM, 120% of initial activity | Hylocereus polyrhizus | |
| 4.2.2.10 | Mg2+ | 10 mM, 112% of initial activity | Hylocereus polyrhizus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.10 | 34000 | - |
gel filtration | Hylocereus polyrhizus |
| 4.2.2.10 | 34200 | - |
1 * 34200, SDS-PAGE | Hylocereus polyrhizus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.2.10 | Hylocereus polyrhizus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.2.10 | - |
Hylocereus polyrhizus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.2.10 | 4-nitrophenyl alpha-D-glucopyranoside | - |
Hylocereus polyrhizus | ? | - |
? | |
| 4.2.2.10 | arabinan | - |
Hylocereus polyrhizus | ? | - |
? | |
| 4.2.2.10 | oat spelt xylan | - |
Hylocereus polyrhizus | ? | - |
? | |
| 4.2.2.10 | polygalacturonic acid | - |
Hylocereus polyrhizus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.2.10 | monomer | 1 * 34200, SDS-PAGE | Hylocereus polyrhizus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.10 | 75 | - |
- |
Hylocereus polyrhizus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.10 | 8 | - |
stable for 1 h | Hylocereus polyrhizus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.10 | 8 | - |
- |
Hylocereus polyrhizus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.10 | 3 | 11 | - |
Hylocereus polyrhizus |
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Release 2023.1 (January 2023)
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