BRENDA - Enzyme Database

Rapid triacylglycerol turnover in Chlamydomonas reinhardtii requires a lipase with broad substrate specificity

Li, X.; Benning, C.; Kuo, M.H.; Eukaryot. Cell 11, 1451-1462 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.1.3
expressed in Escherichia coli BL21-CodonPlus cells
Chlamydomonas reinhardtii
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.3
65000
-
x * 65000, SDS-PAGE
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.3
triacylglycerol + H2O
Chlamydomonas reinhardtii
-
diacylglycerol + a carboxylate
-
-
?
3.1.1.3
triacylglycerol + H2O
Chlamydomonas reinhardtii dw15.1
-
diacylglycerol + a carboxylate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.3
Chlamydomonas reinhardtii
-
-
-
3.1.1.3
Chlamydomonas reinhardtii dw15.1
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.3
Ni-NTA column chromatography
Chlamydomonas reinhardtii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.3
diacylglycerol-N,N,N-trimethylhomoserine + H2O
-
729664
Chlamydomonas reinhardtii
?
-
-
-
?
3.1.1.3
digalactosyldiacylglycerol + H2O
-
729664
Chlamydomonas reinhardtii
?
-
-
-
?
3.1.1.3
monogalactosyldiacylglycerol + H2O
-
729664
Chlamydomonas reinhardtii
?
-
-
-
?
3.1.1.3
monogalactosyldiacylglycerol + H2O
-
729664
Chlamydomonas reinhardtii dw15.1
?
-
-
-
?
3.1.1.3
olive oil + H2O
-
729664
Chlamydomonas reinhardtii
?
-
-
-
?
3.1.1.3
olive oil + H2O
-
729664
Chlamydomonas reinhardtii dw15.1
?
-
-
-
?
3.1.1.3
phosphatidylethanolamine + H2O
-
729664
Chlamydomonas reinhardtii
?
-
-
-
?
3.1.1.3
phosphatidylethanolamine + H2O
-
729664
Chlamydomonas reinhardtii dw15.1
?
-
-
-
?
3.1.1.3
phosphatidylglycerol + H2O
-
729664
Chlamydomonas reinhardtii
?
-
-
-
?
3.1.1.3
phosphatidylglycerol + H2O
-
729664
Chlamydomonas reinhardtii dw15.1
?
-
-
-
?
3.1.1.3
triacylglycerol + H2O
-
729664
Chlamydomonas reinhardtii
diacylglycerol + a carboxylate
-
-
-
?
3.1.1.3
triacylglycerol + H2O
-
729664
Chlamydomonas reinhardtii dw15.1
diacylglycerol + a carboxylate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.3
?
x * 65000, SDS-PAGE
Chlamydomonas reinhardtii
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.3
expressed in Escherichia coli BL21-CodonPlus cells
Chlamydomonas reinhardtii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.3
65000
-
x * 65000, SDS-PAGE
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.3
triacylglycerol + H2O
Chlamydomonas reinhardtii
-
diacylglycerol + a carboxylate
-
-
?
3.1.1.3
triacylglycerol + H2O
Chlamydomonas reinhardtii dw15.1
-
diacylglycerol + a carboxylate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.3
Ni-NTA column chromatography
Chlamydomonas reinhardtii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.3
diacylglycerol-N,N,N-trimethylhomoserine + H2O
-
729664
Chlamydomonas reinhardtii
?
-
-
-
?
3.1.1.3
digalactosyldiacylglycerol + H2O
-
729664
Chlamydomonas reinhardtii
?
-
-
-
?
3.1.1.3
monogalactosyldiacylglycerol + H2O
-
729664
Chlamydomonas reinhardtii
?
-
-
-
?
3.1.1.3
monogalactosyldiacylglycerol + H2O
-
729664
Chlamydomonas reinhardtii dw15.1
?
-
-
-
?
3.1.1.3
olive oil + H2O
-
729664
Chlamydomonas reinhardtii
?
-
-
-
?
3.1.1.3
olive oil + H2O
-
729664
Chlamydomonas reinhardtii dw15.1
?
-
-
-
?
3.1.1.3
phosphatidylethanolamine + H2O
-
729664
Chlamydomonas reinhardtii
?
-
-
-
?
3.1.1.3
phosphatidylethanolamine + H2O
-
729664
Chlamydomonas reinhardtii dw15.1
?
-
-
-
?
3.1.1.3
phosphatidylglycerol + H2O
-
729664
Chlamydomonas reinhardtii
?
-
-
-
?
3.1.1.3
phosphatidylglycerol + H2O
-
729664
Chlamydomonas reinhardtii dw15.1
?
-
-
-
?
3.1.1.3
triacylglycerol + H2O
-
729664
Chlamydomonas reinhardtii
diacylglycerol + a carboxylate
-
-
-
?
3.1.1.3
triacylglycerol + H2O
-
729664
Chlamydomonas reinhardtii dw15.1
diacylglycerol + a carboxylate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.3
?
x * 65000, SDS-PAGE
Chlamydomonas reinhardtii
General Information
EC Number
General Information
Commentary
Organism
3.1.1.3
metabolism
the enzyme facilitates triacylglycerol turnover in Chlamydomonas primarily by degrading the diacylglycerol presumably generated from triacylglycerol hydrolysis
Chlamydomonas reinhardtii
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.1.1.3
metabolism
the enzyme facilitates triacylglycerol turnover in Chlamydomonas primarily by degrading the diacylglycerol presumably generated from triacylglycerol hydrolysis
Chlamydomonas reinhardtii