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Literature summary extracted from

  • Shimanouchi, T.; Kawasaki, H.; Fuse, M.; Umakoshi, H.; Kuboi, R.
    Membrane fusion mediated by phospholipase C under endosomal pH conditions (2013), Colloids Surf. B Biointerfaces, 103, 75-83.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.4.3 pharmacology phospholipase C is considered to be one of key enzymes for the design of drug delivery system using the endocytosis route, because PLC can catalyze the membrane fusion between cell membranes and phospholipid vehicles (liposomes) Bacillus cereus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.3 additional information excess diacylglycerol at pH 7.5 at approximately 12% inhibits the membrane fusion activity of the enzyme Bacillus cereus
3.1.4.3 o-phenanthroline inhibits the phospholipase C hydrolytic activity of the enzyme, but not its membrane fusion activity Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.3 phosphatidylcholine + H2O Bacillus cereus
-
 1,2-sn-diacylglycerol + phosphocholine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Bacillus cereus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.3 commercial preparation
-
 Bacillus cereus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 additional information phospholipase C can catalyze the membrane fusion between cell membranes and phospholipid vehicles (liposomes) at pH 4.0-7.5, highest activity at pH 5.0, 37°C. The liposomes are composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, and cholesterol in a 1:1:1 molar ratio, membrane fusion is dependent of the lipid composition, overview. The enzyme takes a molten-globule state, with a large fluctuation at acidic pH, mechanism of enzyme-induced membrane fusion Bacillus cereus ?
-
 ?
3.1.4.3 phosphatidylcholine + H2O
-
 Bacillus cereus 1,2-sn-diacylglycerol + phosphocholine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.3 More secondary and tertiary structure analysis of the enzyme at different pH values from pH 2.0-7.5, overview Bacillus cereus

Synonyms

EC Number Synonyms Comment Organism
3.1.4.3 PLC
-
 Bacillus cereus

General Information

EC Number General Information Comment Organism
3.1.4.3 additional information secondary and tertiary structure analysis of the enzyme at different pH values from pH 2.0-7.5, overview Bacillus cereus