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Literature summary extracted from

  • Rahman, M.M.; Wang, L.; Inoue, A.; Ojima, T.
    cDNA cloning and bacterial expression of a PL-14 alginate lyase from a herbivorous marine snail Littorina brevicula (2012), Carbohydr. Res., 360, 69-77.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.3 DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Littorina brevicula

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.3 K94A site-directed mutagenesis, the enzyme shows reduced activity compared to the wild-type enzyme Littorina brevicula
4.2.2.3 K97A site-directed mutagenesis, the enzyme shows reduced activity compared to the wild-type enzyme Littorina brevicula
4.2.2.3 R123A site-directed mutagenesis, the enzyme shows reduced activity compared to the wild-type enzyme Littorina brevicula
4.2.2.3 T121A site-directed mutagenesis, the enzyme shows reduced activity compared to the wild-type enzyme, the replacement of T121 by Ala changes the substrate preference of LbAly28 Littorina brevicula
4.2.2.3 Y135A site-directed mutagenesis, the enzyme shows reduced activity compared to the wild-type enzyme Littorina brevicula
4.2.2.3 Y137A site-directed mutagenesis, the enzyme shows reduced activity compared to the wild-type enzyme Littorina brevicula

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.3 28000
-
 x * 28000, recombinant His-tagged wild-type enzyme, SDS-PAGE, x * 28999, sequence calculation Littorina brevicula
4.2.2.3 28999
-
 x * 28000, recombinant His-tagged wild-type enzyme, SDS-PAGE, x * 28999, sequence calculation Littorina brevicula

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.2.3 additional information Littorina brevicula the enzyme is an endolytic polymannuronate lyase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Littorina brevicula G9MBE4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Littorina brevicula

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 additional information the enzyme is an endolytic polymannuronate lyase Littorina brevicula ?
-
 ?
4.2.2.3 poly(beta-D-mannuronate) the region including T121 of LbAly28 is the recognition of poly(MG) region of alginate Littorina brevicula ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 ? x * 28000, recombinant His-tagged wild-type enzyme, SDS-PAGE, x * 28999, sequence calculation Littorina brevicula

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 alginate lyase
-
 Littorina brevicula
4.2.2.3 LbAly28
-
 Littorina brevicula
4.2.2.3 poly(M) lyase
-
 Littorina brevicula

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 30
-
 assay at Littorina brevicula

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 7
-
 assay at Littorina brevicula

General Information

EC Number General Information Comment Organism
4.2.2.3 evolution the enzyme belongs to the family PL-14 Littorina brevicula