Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dou, W.; Wei, D.; Li, H.; Li, H.; Rahman, M.M.; Shi, J.; Xu, Z.; Ma, Y.
    Purification and characterisation of a bifunctional alginate lyase from novel Isoptericola halotolerans CGMCC 5336 (2013), Carbohydr. Polym., 98, 1476-1482.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.3 DNA and amino acid sequence determination and analysis, phylogenetic analysis Isoptericola halotolerans
4.2.2.11 DNA and amino acid sequence determination and analysis, phylogenetic analysis Isoptericola halotolerans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.11 additional information
-
 alginate Km value 0.26 mg/ml, pH 7.0, 30°C Isoptericola halotolerans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.3 Ca2+ activates Isoptericola halotolerans
4.2.2.3 Mg2+ activates Isoptericola halotolerans
4.2.2.3 Mn2+ activates Isoptericola halotolerans
4.2.2.3 Na+ activates Isoptericola halotolerans
4.2.2.11 Ca2+ activates Isoptericola halotolerans
4.2.2.11 Ca2+ 158% of initial activity Isoptericola halotolerans
4.2.2.11 Mg2+ activates Isoptericola halotolerans
4.2.2.11 Mg2+ 173% of initial activity Isoptericola halotolerans
4.2.2.11 Mn2+ activates Isoptericola halotolerans
4.2.2.11 Na+ activates Isoptericola halotolerans
4.2.2.11 Na+ optimum concentration 4 M, about 25% activation Isoptericola halotolerans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.3 28000
-
-
 Isoptericola halotolerans
4.2.2.11 28000
-
-
 Isoptericola halotolerans
4.2.2.11 28000
-
 x * 28000, SDS-PAGE Isoptericola halotolerans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.2.3 alginate Isoptericola halotolerans the bifunctional alginate lyase shows substrate specificity for poly(alpha-L-guluronate) and poly(beta-D-mannuronate) units in alginate molecules, cf. EC 4.2.2.11 ?
-
 ?
4.2.2.3 alginate Isoptericola halotolerans CGMCC5336 the bifunctional alginate lyase shows substrate specificity for poly(alpha-L-guluronate) and poly(beta-D-mannuronate) units in alginate molecules, cf. EC 4.2.2.11 ?
-
 ?
4.2.2.11 alginate Isoptericola halotolerans the bifunctional alginate lyase shows substrate specificity for poly(alpha-L-guluronate) and poly(beta-D-mannuronate) units in alginate molecules, cf. EC 4.2.2.3 ?
-
 ?
4.2.2.11 alginate Isoptericola halotolerans CGMCC5336 the bifunctional alginate lyase shows substrate specificity for poly(alpha-L-guluronate) and poly(beta-D-mannuronate) units in alginate molecules, cf. EC 4.2.2.3 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Isoptericola halotolerans AB489222 isolated from rotten seaweed
-
4.2.2.3 Isoptericola halotolerans CGMCC5336 AB489222 isolated from rotten seaweed
-
4.2.2.11 Isoptericola halotolerans
-
-
-
4.2.2.11 Isoptericola halotolerans AB489222 isolated from rotten seaweed
-
4.2.2.11 Isoptericola halotolerans CGMCC 5336
-
-
-
4.2.2.11 Isoptericola halotolerans CGMCC5336 AB489222 isolated from rotten seaweed
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3 native enzyme 1.75fold by ammonium sulfate fractionation and anion exchange chromatography Isoptericola halotolerans
4.2.2.11 native enzyme 1.75fold by ammonium sulfate fractionation and anion exchange chromatography Isoptericola halotolerans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.3 8409
-
 purified native enzyme, pH 7.0, 50°C Isoptericola halotolerans
4.2.2.11 8409
-
 pH 7.0, 30°C Isoptericola halotolerans
4.2.2.11 8409
-
 purified native enzyme, pH 7.0, 50°C Isoptericola halotolerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alginate the bifunctional alginate lyase shows substrate specificity for poly(alpha-L-guluronate) and poly(beta-D-mannuronate) units in alginate molecules, cf. EC 4.2.2.11 Isoptericola halotolerans ?
-
 ?
4.2.2.3 alginate the bifunctional alginate lyase shows substrate specificity for poly(alpha-L-guluronate) and poly(beta-D-mannuronate) units in alginate molecules, cf. EC 4.2.2.11 Isoptericola halotolerans CGMCC5336 ?
-
 ?
4.2.2.3 poly(beta-D-mannuronate)
-
 Isoptericola halotolerans ?
-
 ?
4.2.2.3 poly(beta-D-mannuronate)
-
 Isoptericola halotolerans CGMCC5336 ?
-
 ?
4.2.2.11 alginate the bifunctional alginate lyase shows substrate specificity for poly(alpha-L-guluronate) and poly(beta-D-mannuronate) units in alginate molecules, cf. EC 4.2.2.3 Isoptericola halotolerans ?
-
 ?
4.2.2.11 alginate enzyme shows specificity for polyguluronate and polymannuronate units in alginate molecules Isoptericola halotolerans ?
-
 ?
4.2.2.11 alginate the bifunctional alginate lyase shows substrate specificity for poly(alpha-L-guluronate) and poly(beta-D-mannuronate) units in alginate molecules, cf. EC 4.2.2.3 Isoptericola halotolerans CGMCC5336 ?
-
 ?
4.2.2.11 alginate enzyme shows specificity for polyguluronate and polymannuronate units in alginate molecules Isoptericola halotolerans CGMCC 5336 ?
-
 ?
4.2.2.11 additional information no substrats: pectin, xanthan, guar gum, arabic gum, sesbania gum, guar gum and carrageenan Isoptericola halotolerans ?
-
 ?
4.2.2.11 additional information no substrats: pectin, xanthan, guar gum, arabic gum, sesbania gum, guar gum and carrageenan Isoptericola halotolerans CGMCC 5336 ?
-
 ?
4.2.2.11 poly(alpha-L-guluronate)
-
 Isoptericola halotolerans ?
-
 ?
4.2.2.11 poly(alpha-L-guluronate)
-
 Isoptericola halotolerans CGMCC5336 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 monomer 1 * 28000 Isoptericola halotolerans
4.2.2.11 ? x * 28000, SDS-PAGE Isoptericola halotolerans
4.2.2.11 monomer 1 * 28000 Isoptericola halotolerans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 50
-
-
 Isoptericola halotolerans
4.2.2.11 50
-
-
 Isoptericola halotolerans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.3 25 60 activity range, profile overview Isoptericola halotolerans
4.2.2.11 25 60 activity range, profile overview Isoptericola halotolerans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.3 40
-
 purified enzyme, loss of 40% activity after 180 min Isoptericola halotolerans
4.2.2.3 50
-
 purified enzyme, inactivation after 120 min Isoptericola halotolerans
4.2.2.3 60
-
 purified enzyme, inactivation after 90 min Isoptericola halotolerans
4.2.2.11 40
-
 stable below Isoptericola halotolerans
4.2.2.11 40
-
 purified enzyme, loss of 40% activity after 180 min Isoptericola halotolerans
4.2.2.11 50
-
 purified enzyme, inactivation after 120 min Isoptericola halotolerans
4.2.2.11 60
-
 purified enzyme, inactivation after 90 min Isoptericola halotolerans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 7
-
-
 Isoptericola halotolerans
4.2.2.11 7
-
-
 Isoptericola halotolerans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.3 5.5 9.5 activity range, profile overview Isoptericola halotolerans
4.2.2.11 5.5 9.5 activity range, profile overview Isoptericola halotolerans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.3 5.5 8.5 purified enzyme, 12 h, over 60% activity within this pH range remains pH 5.5 to 8.5, mostly stable at pH 7.5, half inactivation at pH 9.5 Isoptericola halotolerans
4.2.2.11 5.5 8.5 12 h, more than 60% residual activity Isoptericola halotolerans
4.2.2.11 5.5 8.5 purified enzyme, 12 h, over 60% activity within this pH range remains pH 5.5 to 8.5, mostly stable at pH 7.5, half inactivation at pH 9.5 Isoptericola halotolerans