Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chen, X.; Xu, X.; Jin, Z.; Chen, H.
    Expression of an endoinulinase from Aspergillus ficuum JNSP5-06 in Escherichia coli and its characterization (2012), Carbohydr. Polym., 88, 748-753.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.7 K+ 5 mM, 113% of initial activity Aspergillus ficuum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.7 expression in Escherichia coli Aspergillus ficuum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.7 Ag+ 5 mM, no residual activity Aspergillus ficuum
3.2.1.7 Al3+ 5 mM, 17% residual activity Aspergillus ficuum
3.2.1.7 Fe2+ 5 mM, 30% residual activity Aspergillus ficuum
3.2.1.7 Fe3+ 5 mM, 54% residual activity Aspergillus ficuum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.7 additional information
-
 inulin Km value is 67.4 mg/ml, pH 5.0, 60°C Aspergillus ficuum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.7 Mn2+ 5 mM, 114% of initial activity Aspergillus ficuum
3.2.1.7 Zn2+ 5 mM, 124% of initial activity Aspergillus ficuum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.7 60000
-
 x * 60000, SDS-PAGE Aspergillus ficuum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.7 Aspergillus ficuum
-
-
-
3.2.1.7 Aspergillus ficuum JNSP5-06
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.7 inulin + H2O
-
 Aspergillus ficuum ? major products of hydrolysis are fructo-oligosaccharides with degree of polymerization from 3 to 4 ?
3.2.1.7 inulin + H2O
-
 Aspergillus ficuum JNSP5-06 ? major products of hydrolysis are fructo-oligosaccharides with degree of polymerization from 3 to 4 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.7 ? x * 60000, SDS-PAGE Aspergillus ficuum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.7 Endo I
-
 Aspergillus ficuum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.7 60
-
-
 Aspergillus ficuum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.7 5
-
-
 Aspergillus ficuum