Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kool, M.M.; Schols, H.A.; Wagenknecht, M.; Hinz, S.W.; Moerschbacher, B.M.; Gruppen, H.
    Characterization of an acetyl esterase from Myceliophthora thermophila C1 able to deacetylate xanthan (2014), Carbohydr. Polym., 111, 222-229.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.6 expressed in Escherichia coli Rosetta 2(DE3) cells Thermothelomyces thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.6 Thermothelomyces thermophilus F2X2G0 formerly termed Chrysosporium lucknowense C1
-
3.1.1.6 Thermothelomyces thermophilus C1 F2X2G0 formerly termed Chrysosporium lucknowense C1
-
3.1.1.72 Thermothelomyces thermophilus
-
-
-
3.1.1.72 Thermothelomyces thermophilus C1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.6 Strep-tactin Superflow Plus column chromatography Thermothelomyces thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.72 0.0013
-
 substrate xanthan, pH 5.6, 40°C Thermothelomyces thermophilus
3.1.1.72 8.3
-
 substrate acetylated xylooligosaccharides, pH 5.6, 40°C Thermothelomyces thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.6 xanthan + H2O the enzyme removes 57% of all acetyl groups present in xanthan after a 72 h incubation which corresponds to the removal of about 70% of all acetyl groups positioned on the inner mannose unit Thermothelomyces thermophilus ?
-
 ?
3.1.1.6 xanthan + H2O the enzyme removes 57% of all acetyl groups present in xanthan after a 72 h incubation which corresponds to the removal of about 70% of all acetyl groups positioned on the inner mannose unit Thermothelomyces thermophilus C1 ?
-
 ?
3.1.1.72 acetylated xylooligosaccharides + H2O
-
 Thermothelomyces thermophilus ?
-
 ?
3.1.1.72 acetylated xylooligosaccharides + H2O
-
 Thermothelomyces thermophilus C1 ?
-
 ?
3.1.1.72 xanthan + H2O bacterial polysaccharide xanthan is a polymer having a (1->4)-linked beta-D-glucopyranosyl backbone, with a beta-D-mannopyranosyl-(1->4)-beta-D-glucopyranosyluronic acid-(1->2)-alpha-D-mannopyranosyl side chain substituted to every other glucopyranosyl residue at the O-3 position. Enzyme activity towards xanthan is only observed when xanthan molecules are in the disordered conformation in complete absence of salt. The enzyme specifically removes the acetyl groups positioned on the inner mannose, acetyl groups positioned on the outer mannose are not removed at all. After a prolonged incubation at optimal conditions, 57% of all acetyl groups, representing 70% of all acetyl groups on the inner mannose units, are hydrolyzed Thermothelomyces thermophilus ?
-
 ?
3.1.1.72 xanthan + H2O bacterial polysaccharide xanthan is a polymer having a (1->4)-linked beta-D-glucopyranosyl backbone, with a beta-D-mannopyranosyl-(1->4)-beta-D-glucopyranosyluronic acid-(1->2)-alpha-D-mannopyranosyl side chain substituted to every other glucopyranosyl residue at the O-3 position. Enzyme activity towards xanthan is only observed when xanthan molecules are in the disordered conformation in complete absence of salt. The enzyme specifically removes the acetyl groups positioned on the inner mannose, acetyl groups positioned on the outer mannose are not removed at all. After a prolonged incubation at optimal conditions, 57% of all acetyl groups, representing 70% of all acetyl groups on the inner mannose units, are hydrolyzed Thermothelomyces thermophilus C1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.6 Acetyl esterase
-
 Thermothelomyces thermophilus
3.1.1.6 acetyl xylan esterase
-
 Thermothelomyces thermophilus
3.1.1.6 Axe3
-
 Thermothelomyces thermophilus
3.1.1.72 acetyl xylan esterase
-
 Thermothelomyces thermophilus
3.1.1.72 Axe3
-
 Thermothelomyces thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.6 53
-
 optimal performance towards xanthan at 53°C Thermothelomyces thermophilus
3.1.1.72 53
-
 with xanthan Thermothelomyces thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.6 50 55
-
 Thermothelomyces thermophilus

General Information

EC Number General Information Comment Organism
3.1.1.72 evolution the enzyme belongs to the carbohydrate esterase family 1, CE1 Thermothelomyces thermophilus