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Literature summary extracted from

  • Salmon, D.N.; Piva, L.C.; Binati, R.L.; Rodrigues, C.; Vandenberghe, L.P.; Soccol, C.R.; Spier, M.R.
    A bioprocess for the production of phytase from Schizophyllum commune: studies of its optimization, profile of fermentation parameters, characterization and stability (2012), Bioprocess Biosyst. Eng., 35, 1067-1079.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.8 acetate 1 mM, 136% of initial activity Schizophyllum commune
3.1.3.8 citrate 1 mM, 121% of initial activity Schizophyllum commune

Application

EC Number Application Comment Organism
3.1.3.8 synthesis a maximal level of phytase of 113.7 U/g of dry substrate is obtained in wheat bran based medium containing 5% sucrose, 50% humidity, 7.5% of biomass at 33°C, pH 7.0 during 72 h and a 285% improvement in enzyme titre is achieved Schizophyllum commune

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.16
-
 myo-inositol hexakisphosphate pH 5.0, 50°C Schizophyllum commune

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.8 CaSO4 1 mM, 343% of initial activity Schizophyllum commune
3.1.3.8 CuSO4 1 mM, 274% of initial activity Schizophyllum commune
3.1.3.8 Fe2(SO4)3 1 mM, 308% of initial activity Schizophyllum commune
3.1.3.8 FeSO4 1 mM, 293% of initial activity Schizophyllum commune
3.1.3.8 K2SO4 1 mM, 356% of initial activity Schizophyllum commune
3.1.3.8 MgSO4 1 mM, 316% of initial activity Schizophyllum commune
3.1.3.8 MnSO4 1 mM, 369% of initial activity Schizophyllum commune
3.1.3.8 Na2SO4 1 mM, 295% of initial activity Schizophyllum commune
3.1.3.8 NiSO4 1 mM, 285% of initial activity Schizophyllum commune
3.1.3.8 ZnSO4 1 mM, 211% of initial activity Schizophyllum commune

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Schizophyllum commune
-
-
-
3.1.3.8 Schizophyllum commune LPB 101
-
-
-

Storage Stability

EC Number Storage Stability Organism
3.1.3.8 4°C, best shelf life, maintaining 38% of its initial activity after 112 days Schizophyllum commune
3.1.3.8 stabilization by PEG 3350 results in 109% of phytase activity from the initial crude extract remaining after 90 days Schizophyllum commune

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Schizophyllum commune myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
 Schizophyllum commune LPB 101 myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 50
-
-
 Schizophyllum commune

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5
-
-
 Schizophyllum commune