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Literature summary extracted from

  • Holewinski, R.J.; Creighton, D.J.
    Inhibition by active site directed covalent modification of human glyoxalase I (2014), Bioorg. Med. Chem., 22, 3301-3308.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.5 recombinant expression in Escherichia coli strain JM103 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.5 4-bromoacetoxy-1-(S-glutathionyl)-acetoxy butane competitive inhibition of GLO1, the inhibitor is able to covalently bind to the free sulfhydryl group of Cys60 in the hydrophobic binding pocket adjacent to the enzyme active site and partially inactivate the enzyme, no complete inhibition, binding structure analysis, overview Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.5 0.182
-
 methylglyoxal recombinant enzyme, pH 7.3, 25°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.5 20647
-
 2 * 20647, sequence calculation, 2 * 21000, recombinant enzyme, SDS-PAGE Homo sapiens
4.4.1.5 21000
-
 2 * 20647, sequence calculation, 2 * 21000, recombinant enzyme, SDS-PAGE Homo sapiens
4.4.1.5 42000
-
 reombinant enzyme, native PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.5 glutathione + methylglyoxal Homo sapiens
-
 (R)-S-lactoylglutathione
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.5 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.5 recombinant enzyme from Escherichia coli strain JM103 by S-hexylglutathione affinity chromatography and gel filtration Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.5 1580
-
 purified recombinant enzyme, pH 7.3, 25°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.5 glutathione + methylglyoxal
-
 Homo sapiens (R)-S-lactoylglutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.5 homodimer 2 * 20647, sequence calculation, 2 * 21000, recombinant enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
4.4.1.5 Glo1
-
 Homo sapiens
4.4.1.5 glyoxalase I
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.5 25
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.5 1088
-
 methylglyoxal recombinant enzyme, pH 7.3, 25°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.5 7.3 7.4 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
4.4.1.5 metabolism glyoxalase I is the first enzyme of the methylglyoxal detoxification pathway Homo sapiens
4.4.1.5 physiological function the glyoxalase pathway is responsible for conversion of cytotoxic methylglyoxal to D-lactate. Methylglyoxal toxicity arises from its ability to form advanced glycation end products on proteins, lipids and DNA Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.4.1.5 0.006
-
 methylglyoxal recombinant enzyme, pH 7.3, 25°C Homo sapiens