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Literature summary extracted from
- A major phospholipase A2 from Daboia russelii russelii venom shows potent anticoagulant action via thrombin inhibition and binding with plasma phospholipids (2014), Biochimie, 99, 153-161.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.4 | p-bromophenacyl bromide | inhibits both the catalytic and anticoagulant activities. Inhibition of catalytic activity is approximately 6fold higher compared with inhibition of anticoagulant activity | Daboia russelii |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.4 | 0.61 | - |
phosphatidylcholine | pH 8.0, 37°C | Daboia russelii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.4 | 13840 | - |
MALDI-TOF mass spectrometry | Daboia russelii |
| 3.1.1.4 | 14000 | - |
PAGE | Daboia russelii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.4 | Daboia russelii | - |
subsp. russelii | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.4 | - |
Daboia russelii |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.4 | venom | - |
Daboia russelii | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.1.4 | -20°C, five cycles of freezing-thawing do not affect the catalytic or anticoagulant activity | Daboia russelii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.4 | 4-nitro-3-(octanoyloxy) benzoic acid + H2O | - |
Daboia russelii | ? | - |
? | |
| 3.1.1.4 | phosphatidylcholine + H2O | - |
Daboia russelii | 1-acylglycerophosphocholine + fatty acid | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.4 | monomer | 1* 13835, MALDI-TOF mass spectrometry, 1 * 14000, SDS-PAGE | Daboia russelii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.4 | 37 | 45 | - |
Daboia russelii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.4 | 8 | 9 | - |
Daboia russelii |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.1.1.4 | physiological function | enzyme shows strong anticoagulant effect via thrombin inhibition with a Ki value of 380 nM as well as by binding to pro-coagulant phospholipids of plasma. In ex-vivo conditionsthe enzyme at 1.0 microM is non-hemolytic and non-cytotoxic to mammalian cells. It does not inhibit the collagen-induced aggregation of platelets. At a dose of 5 mg/kg, the enzyme is not lethal to mice after 48 h of injection | Daboia russelii |
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Release 2023.1 (January 2023)
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