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Literature summary extracted from

  • Martínez-Espinosa, R.M.; Richardson, D.J.; Bonete, M.J.
    Characterisation of chlorate reduction in the haloarchaeon Haloferax mediterranei (2015), Biochim. Biophys. Acta, 1850, 587-594.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.97.1.1 2.4
-
 Chlorate pH 8, 40°C Haloferax mediterranei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.97.1.1 NaCl the maximum specific chlorate reductase activity is detected in the presence of low NaCl concentrations at 35 °C. At salt concentrations higher than 0.4 M, the maximum specific activity can be detected at higher temperatures (between 40°C and 45°C) Haloferax mediterranei

Organism

EC Number Organism UniProt Comment Textmining
1.7.7.2 Haloferax mediterranei I3R9M9 and I3R9M8 I3R9M9: alpha-subunit (narG), I3R9M8: beta subunit (narH)
-
1.7.7.2 Haloferax mediterranei ATCC 33500 I3R9M9 and I3R9M8 I3R9M9: alpha-subunit (narG), I3R9M8: beta subunit (narH)
-
1.97.1.1 Haloferax mediterranei I3R9M9 and I3R9M8 I3R9M9: alpha-subunit (narG), I3R9M8: beta subunit (narH)
-
1.97.1.1 Haloferax mediterranei ATCC 33500 I3R9M9 and I3R9M8 I3R9M9: alpha-subunit (narG), I3R9M8: beta subunit (narH)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.7.2
-
 Haloferax mediterranei
1.97.1.1
-
 Haloferax mediterranei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.7.2 nitrite + acceptor
-
 Haloferax mediterranei nitrate + reduced acceptor
-
 ?
1.7.7.2 nitrite + acceptor
-
 Haloferax mediterranei ATCC 33500 nitrate + reduced acceptor
-
 ?
1.97.1.1 chlorate + reduced methyl viologen
-
 Haloferax mediterranei chlorite + H2O + methyl viologen
-
 ?
1.97.1.1 chlorate + reduced methyl viologen
-
 Haloferax mediterranei ATCC 33500 chlorite + H2O + methyl viologen
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.7.2 NarGH
-
 Haloferax mediterranei
1.97.1.1 NarGH
-
 Haloferax mediterranei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.97.1.1 35
-
 the maximum specific chlorate reductase activity is detected in the presence of low NaCl concentrations at 35°C. At salt concentrations higher than 0.4 M, the maximum specific activity can be detected at higher temperatures (between 40°C and 45°C) Haloferax mediterranei
1.97.1.1 40 45 the maximum specific chlorate reductase activity is detected in the presence of low NaCl concentrations at 35°C. At salt concentrations higher than 0.4 M, the maximum specific activity can be detected at higher temperatures (between 40°C and 45°C) Haloferax mediterranei
1.97.1.1 40
-
 assay at Haloferax mediterranei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.97.1.1 46
-
 Chlorate pH 8, 40°C Haloferax mediterranei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.97.1.1 8
-
-
 Haloferax mediterranei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.97.1.1 5.5 9 pH 5.0: about 85% of maximal activity, pH 9.0: about 75% of maximal activity Haloferax mediterranei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.97.1.1 19
-
 Chlorate pH 8, 40°C Haloferax mediterranei