EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.18 | expressed in Escherichia coli BL21(DE3) cells | Aspergillus fumigatus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.18 | D84A | the mutation significantly lowers the activity of the enzyme, but this mutant remains a retaining glycosidase | Aspergillus fumigatus |
3.2.1.18 | R171L | the mutant has higher activity on a N-acetylneuraminic acid-based substrate than wild type enzyme | Aspergillus fumigatus |
3.2.1.18 | Y358H | the mutation significantly lowers the activity of the enzyme, but this mutant remains a retaining glycosidase | Aspergillus fumigatus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.18 | Aspergillus fumigatus | Q4WQS0 | - |
- |
3.2.1.18 | Aspergillus fumigatus Af293 | Q4WQS0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.18 | Ni-NTA column chromatography and Sephacryl S-100H gel filtration | Aspergillus fumigatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.18 | 4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid + H2O | preferred substrate | Aspergillus fumigatus | ? | - |
? | |
3.2.1.18 | 4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid + H2O | preferred substrate | Aspergillus fumigatus Af293 | ? | - |
? | |
3.2.1.18 | 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O | - |
Aspergillus fumigatus | ? | - |
? | |
3.2.1.18 | 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O | - |
Aspergillus fumigatus Af293 | ? | - |
? | |
3.2.1.18 | 8-fluoromethylumbelliferyl alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside + H2O | - |
Aspergillus fumigatus | 8-fluoromethylumbelliferyl alpha-D-galactopyranoside + 3-deoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosonate | - |
? | |
3.2.1.18 | 8-fluoromethylumbelliferyl alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside + H2O | - |
Aspergillus fumigatus Af293 | 8-fluoromethylumbelliferyl alpha-D-galactopyranoside + 3-deoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosonate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.18 | KDNase | - |
Aspergillus fumigatus |
3.2.1.18 | sialidase | - |
Aspergillus fumigatus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.18 | 8 | - |
8-fluoromethylumbelliferyl alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside | wild type enzyme, pH 5.2, 16 mM sodium tartrate, at 37°C | Aspergillus fumigatus | |
3.2.1.18 | 200 | - |
8-fluoromethylumbelliferyl alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside | mutant enzyme D84A, pH 5.2, 16 mM sodium tartrate, at 37°C | Aspergillus fumigatus | |
3.2.1.18 | 330 | - |
4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid | mutant enzyme R71L, pH 5.2, 16 mM sodium tartrate, at 37°C | Aspergillus fumigatus | |
3.2.1.18 | 1620 | - |
8-fluoromethylumbelliferyl alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside | mutant enzyme R71L, pH 5.2, 16 mM sodium tartrate, at 37°C | Aspergillus fumigatus | |
3.2.1.18 | 8900 | - |
8-fluoromethylumbelliferyl alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside | mutant enzyme Y358H, pH 5.2, 16 mM sodium tartrate, at 37°C | Aspergillus fumigatus | |
3.2.1.18 | 11600 | - |
4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid | mutant enzyme D84A, pH 5.2, 16 mM sodium tartrate, at 37°C | Aspergillus fumigatus | |
3.2.1.18 | 14500 | - |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid | mutant enzyme Y358H, pH 5.2, 16 mM sodium tartrate, at 37°C | Aspergillus fumigatus | |
3.2.1.18 | 19000 | - |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid | mutant enzyme D84A, pH 5.2, 16 mM sodium tartrate, at 37°C | Aspergillus fumigatus | |
3.2.1.18 | 26800 | - |
4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid | mutant enzyme Y358H, pH 5.2, 16 mM sodium tartrate, at 37°C | Aspergillus fumigatus | |
3.2.1.18 | 47700 | - |
4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid | wild type enzyme, pH 5.2, 16 mM sodium tartrate, at 37°C | Aspergillus fumigatus | |
3.2.1.18 | 53500 | - |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid | wild type enzyme, pH 5.2, 16 mM sodium tartrate, at 37°C | Aspergillus fumigatus | |
3.2.1.18 | 74200 | - |
4-methylumbelliferyl alpha-D-N-acetylneuraminic acid | mutant enzyme R71L, pH 5.2, 16 mM sodium tartrate, at 37°C | Aspergillus fumigatus |