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Literature summary extracted from

  • Daughtry, K.D.; Huang, H.; Malashkevich, V.; Patskovsky, Y.; Liu, W.; Ramagopal, U.; Sauder, J.M.; Burley, S.K.; Almo, S.C.; Dunaway-Mariano, D.; Allen, K.N.
    Structural basis for the divergence of substrate specificity and biological function within HAD phosphatases in lipopolysaccharide and sialic acid biosynthesis (2013), Biochemistry, 52, 5372-5386.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.45 expressed in Escherichia coli BL21(DE3) cells Bacteroides thetaiotaomicron
3.1.3.45 expressed in Escherichia coli BL21(DE3) cells Haemophilus influenzae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.45 enzyme bound to Mg2+ and metavanadate, hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol MME 550, 40 mM MgCl2 and 100 mM HEPES (pH 7.5), at 18°C Bacteroides thetaiotaomicron
3.1.3.45 enzyme bound to Mg2+ and metavanadate, hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol MME 550, 40 mM MgCl2 and 100 mM HEPES (pH 7.5), at 18°C Haemophilus influenzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.45 0.0001
-
 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate at pH 7.0 and 25°C Bacteroides thetaiotaomicron
3.1.3.45 0.0009
-
 KDN 9-phosphate at pH 7.0 and 25°C Bacteroides thetaiotaomicron
3.1.3.45 0.16
-
 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate at pH 7.0 and 25°C Haemophilus influenzae
3.1.3.45 1.6
-
 KDN 9-phosphate at pH 7.0 and 25°C Haemophilus influenzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.45 Mg2+ required Bacteroides thetaiotaomicron
3.1.3.45 Mg2+ required Haemophilus influenzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.45 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O Bacteroides thetaiotaomicron best substrate ? + phosphate
-
 ?
3.1.3.45 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O Haemophilus influenzae best substrate ? + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.45 Bacteroides thetaiotaomicron
-
-
-
3.1.3.45 Haemophilus influenzae P45314
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.45 TALON metal affinity resin column chromatography Bacteroides thetaiotaomicron
3.1.3.45 TALON metal affinity resin column chromatography Haemophilus influenzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.45 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O
-
 Bacteroides thetaiotaomicron ? + phosphate
-
 ?
3.1.3.45 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O
-
 Haemophilus influenzae ? + phosphate
-
 ?
3.1.3.45 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O best substrate Bacteroides thetaiotaomicron ? + phosphate
-
 ?
3.1.3.45 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate + H2O best substrate Haemophilus influenzae ? + phosphate
-
 ?
3.1.3.45 KDN 9-phosphate + H2O
-
 Bacteroides thetaiotaomicron 3-deoxy-D-glycero-beta-D-galacto-non-2-ulopyranosonate + phosphate
-
 ?
3.1.3.45 KDN 9-phosphate + H2O
-
 Haemophilus influenzae 3-deoxy-D-glycero-beta-D-galacto-non-2-ulopyranosonate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.45 dimer x-ray crystallography Bacteroides thetaiotaomicron
3.1.3.45 dimer x-ray crystallography Haemophilus influenzae

Synonyms

EC Number Synonyms Comment Organism
3.1.3.45 2-keto-3-deoxy-D-manno-octulosonate 8-phosphate phosphohydrolase
-
 Bacteroides thetaiotaomicron
3.1.3.45 2-keto-3-deoxy-D-manno-octulosonate 8-phosphate phosphohydrolase
-
 Haemophilus influenzae
3.1.3.45 KDO8P phosphatase
-
 Bacteroides thetaiotaomicron
3.1.3.45 KDO8P phosphatase
-
 Haemophilus influenzae
3.1.3.45 KDO8PP
-
 Bacteroides thetaiotaomicron
3.1.3.45 KDO8PP
-
 Haemophilus influenzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.45 0.021
-
 KDN 9-phosphate at pH 7.0 and 25°C Haemophilus influenzae
3.1.3.45 0.061
-
 KDN 9-phosphate at pH 7.0 and 25°C Bacteroides thetaiotaomicron
3.1.3.45 0.78
-
 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate at pH 7.0 and 25°C Haemophilus influenzae
3.1.3.45 1.5
-
 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate at pH 7.0 and 25°C Bacteroides thetaiotaomicron

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.45 0.013
-
 KDN 9-phosphate at pH 7.0 and 25°C Haemophilus influenzae
3.1.3.45 0.069
-
 KDN 9-phosphate at pH 7.0 and 25°C Bacteroides thetaiotaomicron
3.1.3.45 4.8
-
 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate at pH 7.0 and 25°C Haemophilus influenzae
3.1.3.45 15
-
 2-oxo-3-deoxy-D-manno-octulosonate 8-phosphate at pH 7.0 and 25°C Bacteroides thetaiotaomicron