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Literature summary extracted from

  • Farrell, E.K.; Tipton, P.A.
    Functional characterization of AlgL, an alginate lyase from Pseudomonas aeruginosa (2012), Biochemistry, 51, 10259-10266.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.3 gene algL, algL is part of the alginate biosynthetic operon, expression of His-tagged enzyme in Escherichia coli strain C41(DE3) Pseudomonas aeruginosa
4.2.2.11 expression in Escherichia coli Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.3 additional information
-
 additional information steady-state kinetics, overview Pseudomonas aeruginosa
4.2.2.11 0.0026
-
 acetylated alginate pH 7.1, 25°C Pseudomonas aeruginosa
4.2.2.11 0.005
-
 acetylated poly-(beta-(1->4)-D-mannuronan) pH 7.1, 25°C Pseudomonas aeruginosa
4.2.2.11 0.011
-
 alginate pH 7.1, 25°C Pseudomonas aeruginosa
4.2.2.11 0.013
-
 poly-(beta-(1->4)-D-mannuronan) pH 7.1, 25°C Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.2.3 additional information Pseudomonas aeruginosa alginate lyase AlgL catalyzes the cleavage of the polysaccharide alginate through a beta-elimination reaction. AlgL operates preferentially on non-acetylated alginate or its precursor mannuronan. AlgL operates as an exopolysaccharide lyase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Pseudomonas aeruginosa Q06749 gene algL
-
4.2.2.11 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3 recombinant His-tagged enzyme from Escherichia coli strain C41(DE3) by nickel or cobalt affinity chromatography Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
4.2.2.11 -80°C, presence of 10% glycerol, stable for several months Pseudomonas aeruginosa
4.2.2.11 4°C, stable for 2 weeks Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alginate AlgL lacks of stereospecificity, in that it is able to catalyze cleavage adjacent to either mannuronate or guluronate residues in alginate. The enzyme is able to remove the C5 proton from both mannuronate and guluronate, which are C5 epimers. Exhaustive digestion of alginate by AlgL generates dimeric and trimeric products, analysis by 1H NMR spectroscopy and mass spectrometry. AlgL operates as an exopolysaccharide lyase Pseudomonas aeruginosa ?
-
 ?
4.2.2.3 additional information alginate lyase AlgL catalyzes the cleavage of the polysaccharide alginate through a beta-elimination reaction. AlgL operates preferentially on non-acetylated alginate or its precursor mannuronan. AlgL operates as an exopolysaccharide lyase Pseudomonas aeruginosa ?
-
 ?
4.2.2.11 acetylated alginate
-
 Pseudomonas aeruginosa ?
-
 ?
4.2.2.11 acetylated poly-(beta-(1->4)-D-mannuronan)
-
 Pseudomonas aeruginosa ?
-
 ?
4.2.2.11 alginate
-
 Pseudomonas aeruginosa ?
-
 ?
4.2.2.11 additional information operates in a processive manner. It is able to catalyze cleavage adjacent to either mannuronate or guluronate residues in alginate Pseudomonas aeruginosa ?
-
 ?
4.2.2.11 poly-(beta-(1->4)-D-mannuronan) Km value decreases with increasing substrate length, and kcat/Km increases. Oligomers containing fewer than 9-10 residues are not substrates Pseudomonas aeruginosa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 alginate lyase
-
 Pseudomonas aeruginosa
4.2.2.3 AlgL
-
 Pseudomonas aeruginosa
4.2.2.11 AlgL
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 25
-
 assay at Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.2.11 1.2
-
 acetylated alginate pH 7.1, 25°C Pseudomonas aeruginosa
4.2.2.11 1.5
-
 acetylated poly-(beta-(1->4)-D-mannuronan) pH 7.1, 25°C Pseudomonas aeruginosa
4.2.2.11 32
-
 alginate pH 7.1, 25°C Pseudomonas aeruginosa
4.2.2.11 32
-
 poly-(beta-(1->4)-D-mannuronan) pH 7.1, 25°C Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 7.1
-
 assay at Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
4.2.2.3 metabolism AlgL is required for for alginate biosynthesis Pseudomonas aeruginosa
4.2.2.3 physiological function AlgL serves to degrade mislocalized alginate that is trapped in the periplasmic space Pseudomonas aeruginosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.2.11 300
-
 acetylated poly-(beta-(1->4)-D-mannuronan) pH 7.1, 25°C Pseudomonas aeruginosa
4.2.2.11 500
-
 acetylated alginate pH 7.1, 25°C Pseudomonas aeruginosa
4.2.2.11 2500
-
 poly-(beta-(1->4)-D-mannuronan) pH 7.1, 25°C Pseudomonas aeruginosa
4.2.2.11 3100
-
 alginate pH 7.1, 25°C Pseudomonas aeruginosa