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Literature summary extracted from

  • Lu, H.; Tsai, S.; Khosla, C.; Cane, D.
    Expression, site-directed mutagenesis, and steady state kinetic analysis of the terminal thioesterase domain of the methymycin/picromycin polyketide synthase (2002), Biochemistry, 41, 12590-12597.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.239 the thioesterase domain of the methymycin/picromycin synthase (PICS) is functionally expressed in Escherichia coli Streptomyces venezuelae
2.3.1.240 the thioesterase domain of the methymycin/picromycin synthase (PICS) is functionally expressed in Escherichia coli Streptomyces venezuelae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.239 additional information
-
 additional information steady state kinetic parameters for diketides with wild-type and mutant thioesterase domain of methymycin/picromycin synthase Streptomyces venezuelae
2.3.1.240 additional information
-
 additional information steady state kinetic parameters for diketides with wild-type and mutant thioesterase domain of methymycin/picromycin synthase Streptomyces venezuelae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.239 malonyl-CoA + 5 (2S)-methylmalonyl-CoA + 5 NADPH + 5 H+ Streptomyces venezuelae the product 10-deoxymethynolide is an intermediate in the biosynthesis of methymycin 10-deoxymethynolide + 6 CoA + 6 CO2 + 5 NADP+ + 2 H2O
-
 ?
2.3.1.240 malonyl-CoA + 6 (2S)-methylmalonyl-CoA + 5 NADPH + 5 H+ Streptomyces venezuelae the product narbonolide is an intermediate in the biosynthesis of methymycin narbonolide + 7 CoA + 7 CO2 + 5 NADP+ + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.239 Streptomyces venezuelae
-
-
-
2.3.1.240 Streptomyces venezuelae Q9ZGI5 and Q9ZGI4 and Q9ZGI3 and Q9ZGI2 and Q9ZGI1 Q9ZGI5: type I polyketide synthase PikAI, Q9ZGI4: type I polyketide synthase PikAII, Q9ZGI3: type I polyketide synthase PikAIII, Q9ZGI2: type I polyketide synthase PikAIV, Q9ZGI1: thioesterase II PikAV
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.239 malonyl-CoA + 5 (2S)-methylmalonyl-CoA + 5 NADPH + 5 H+ the product 10-deoxymethynolide is an intermediate in the biosynthesis of methymycin Streptomyces venezuelae 10-deoxymethynolide + 6 CoA + 6 CO2 + 5 NADP+ + 2 H2O
-
 ?
2.3.1.239 malonyl-CoA + 5 (2S)-methylmalonyl-CoA + 5 NADPH + 5 H+ the enzyme also produces narbonolide (see EC 2.3.1.240, narbonolide synthase). The enzyme has 29 active sites arranged in four polypeptides (pikAI - pikAIV) with a loading domain, six extension modules and a terminal thioesterase domain. Each extension module contains a ketosynthase (KS), keto reductase (KR), an acyltransferase (AT) and an acyl-carrier protein (ACP). Not all active sites are used in the biosynthesis Streptomyces venezuelae 10-deoxymethynolide + 6 CoA + 6 CO2 + 5 NADP+ + 2 H2O
-
 ?
2.3.1.240 malonyl-CoA + 6 (2S)-methylmalonyl-CoA + 5 NADPH + 5 H+ the product narbonolide is an intermediate in the biosynthesis of methymycin Streptomyces venezuelae narbonolide + 7 CoA + 7 CO2 + 5 NADP+ + 2 H2O
-
 ?
2.3.1.240 malonyl-CoA + 6 (2S)-methylmalonyl-CoA + 5 NADPH + 5 H+ the enzyme also produces 10-deoxymethynolide (see EC 2.3.1.239, 10-deoxymethynolide synthase). The enzyme has 29 active sites arranged in four polypeptides (pikAI - pikAIV) with a loading domain, six extension modules and a terminal thioesterase domain. Each extension module contains a ketosynthase (KS), keto reductase (KR), an acyltransferase (AT) and an acyl-carrier protein (ACP). Not all active sites are used in the biosynthesis Streptomyces venezuelae narbonolide + 7 CoA + 7 CO2 + 5 NADP+ + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.239 methymycin/picromycin polyketide synthase
-
 Streptomyces venezuelae
2.3.1.239 PICS
-
 Streptomyces venezuelae
2.3.1.240 methymycin/picromycin polyketide synthase
-
 Streptomyces venezuelae
2.3.1.240 PICS
-
 Streptomyces venezuelae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.239 additional information
-
 additional information steady state kinetic parameters for diketides with wild-type and mutant thioesterase domain of methymycin/picromycin synthase Streptomyces venezuelae
2.3.1.240 additional information
-
 additional information steady state kinetic parameters for diketides with wild-type and mutant thioesterase domain of methymycin/picromycin synthase Streptomyces venezuelae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.239 additional information
-
 pH-dependence of the hydrolysis of thioesters by the thioesterase domain of methymycin/picromycin synthase with diketides Streptomyces venezuelae
2.3.1.240 additional information
-
 pH-dependence of the hydrolysis of thioesters by the thioesterase domain of methymycin/picromycin synthase with diketides Streptomyces venezuelae

General Information

EC Number General Information Comment Organism
2.3.1.239 metabolism the enzyme is involved in the biosynthesis of methymycin Streptomyces venezuelae
2.3.1.240 metabolism the enzyme is involved in the biosynthesis of narbonomycin Streptomyces venezuelae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.239 additional information
-
 additional information steady state kinetic parameters for diketides with wild-type and mutant thioesterase domain of methymycin/picromycin synthase Streptomyces venezuelae
2.3.1.240 additional information
-
 additional information steady state kinetic parameters for diketides with wild-type and mutant thioesterase domain of methymycin/picromycin synthase Streptomyces venezuelae