BRENDA - Enzyme Database

Picking sides: Distinct roles for CYP76M6 and CYP76M8 in rice oryzalexin biosynthesis

Wu, Y.; Wang, Q.; Hillwig, M.; Peters, R.; Biochem. J. 454, 209-216 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.14.70
expressed in Escherichia coli C41 cells
Oryza sativa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.70
ent-sandaracopimaradiene + NADPH + H+ + O2
Oryza sativa
-
ent-sandaracopimaradien-3beta-ol + NADP+ + H2O
-
-
?
1.14.14.122
ent-sandaracopimaradien-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa
-
oryzalexin E + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.123
ent-sandaracopimaradien-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa
-
oryzalexin D + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.70
Oryza sativa
-
-
-
1.14.14.122
Oryza sativa
-
-
-
1.14.14.123
Oryza sativa
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.70
7beta-hydroxy-ent-sandaracopimaradiene + [reduced NADPH-hemoprotein reductase] + O2
poor reaction
729163
Oryza sativa
oryzalexin D + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.70
ent-sandaracopimaradiene + NADPH + H+ + O2
-
729163
Oryza sativa
ent-sandaracopimaradien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.14.122
ent-sandaracopimaradien-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2
-
729163
Oryza sativa
oryzalexin E + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.123
ent-sandaracopimaradien-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2
-
729163
Oryza sativa
oryzalexin D + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.70
NADPH
-
Oryza sativa
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.70
expressed in Escherichia coli C41 cells
Oryza sativa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.70
NADPH
-
Oryza sativa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.70
ent-sandaracopimaradiene + NADPH + H+ + O2
Oryza sativa
-
ent-sandaracopimaradien-3beta-ol + NADP+ + H2O
-
-
?
1.14.14.122
ent-sandaracopimaradien-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa
-
oryzalexin E + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.123
ent-sandaracopimaradien-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa
-
oryzalexin D + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.70
7beta-hydroxy-ent-sandaracopimaradiene + [reduced NADPH-hemoprotein reductase] + O2
poor reaction
729163
Oryza sativa
oryzalexin D + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.70
ent-sandaracopimaradiene + NADPH + H+ + O2
-
729163
Oryza sativa
ent-sandaracopimaradien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.14.122
ent-sandaracopimaradien-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2
-
729163
Oryza sativa
oryzalexin E + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.123
ent-sandaracopimaradien-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2
-
729163
Oryza sativa
oryzalexin D + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-