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Literature summary extracted from
- Super-perfect enzymes: Structural stabilities and activities of recombinant triose phosphate isomerases from Pyrococcus furiosus and Thermococcus onnurineus produced in Escherichia coli (2015), Biochem. Biophys. Res. Commun., 460, 753-758.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.1 | expression in Escherichia coli | Thermococcus onnurineus |
| 5.3.1.1 | expression in Escherichia coli | Pyrococcus furiosus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.1 | 0.21 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 90°C | Thermococcus onnurineus |  |
| 5.3.1.1 | 0.68 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 90°C | Pyrococcus furiosus | |
| 5.3.1.1 | 1.2 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 25°C | Pyrococcus furiosus | |
| 5.3.1.1 | 1.67 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 25°C | Thermococcus onnurineus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.1 | 25000 | - |
4 * 25000, SDS-PAGE | Thermococcus onnurineus |
| 5.3.1.1 | 25000 | - |
4 * 25000, SDS-PAGE | Pyrococcus furiosus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.1 | Pyrococcus furiosus | P62002 | - |
- |
| 5.3.1.1 | Thermococcus onnurineus | B6YUE5 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.1.1 | - |
Thermococcus onnurineus |
| 5.3.1.1 | - |
Pyrococcus furiosus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.1 | D-Glyceraldehyde 3-phosphate | - |
Thermococcus onnurineus | Glycerone phosphate | - |
? | |
| 5.3.1.1 | D-Glyceraldehyde 3-phosphate | - |
Pyrococcus furiosus | Glycerone phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.1.1 | tetramer | 4 * 25000, SDS-PAGE | Thermococcus onnurineus |
| 5.3.1.1 | tetramer | 4 * 25000, SDS-PAGE | Pyrococcus furiosus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.1.1 | PfuTIM | - |
Pyrococcus furiosus |
| 5.3.1.1 | TonTIM | - |
Thermococcus onnurineus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.1 | 90 | - |
no structural unfolding at 90°C | Thermococcus onnurineus |
| 5.3.1.1 | 90 | - |
no structural unfolding at 90°C | Pyrococcus furiosus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.1 | 1430000 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 25°C | Thermococcus onnurineus | |
| 5.3.1.1 | 1548000 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 25°C | Pyrococcus furiosus | |
| 5.3.1.1 | 25000000 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 90°C | Thermococcus onnurineus | |
| 5.3.1.1 | 57500000 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 90°C | Pyrococcus furiosus | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.1 | 857000 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 25°C | Thermococcus onnurineus | |
| 5.3.1.1 | 1269000 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 25°C | Pyrococcus furiosus | |
| 5.3.1.1 | 84880000 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 90°C | Pyrococcus furiosus | |
| 5.3.1.1 | 120400000 | - |
D-glyceraldehyde 3-phosphate | pH 8.0, 90°C | Thermococcus onnurineus | |
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