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Literature summary extracted from

  • Wagner, M.; Andreesen, J.R.
    Purification and characterization of threonine dehydrogenase from Clostridium sticklandi (1995), Arch. Microbiol., 163, 286-290.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.103 Co2+ 0.9 mM, 61% inhibiton Acetoanaerobium sticklandii
1.1.1.103 Cu2+ 0.9 mM, 54% inhibiton Acetoanaerobium sticklandii
1.1.1.103 additional information no inhibition by Ni2+, Ca2+, Mg2+, Mn2+ Acetoanaerobium sticklandii
1.1.1.103 Zn2+ 0.9 mM, 23% inhibiton Acetoanaerobium sticklandii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.103 0.1
-
 NAD+ pH 9.0, 37°C Acetoanaerobium sticklandii
1.1.1.103 18
-
 L-threonine pH 9.0, 37°C Acetoanaerobium sticklandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.103 35000
-
 gel filtration, monomeric enzyme Acetoanaerobium sticklandii
1.1.1.103 80000
-
 gel filtration, dimeric enzyme Acetoanaerobium sticklandii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.103 L-threonine + NAD+ Acetoanaerobium sticklandii
-
 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?
1.1.1.103 L-threonine + NAD+ Acetoanaerobium sticklandii DSM 519
-
 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.103 Acetoanaerobium sticklandii E3PS87
-
-
1.1.1.103 Acetoanaerobium sticklandii DSM 519 E3PS87
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.103 chromatography on Q-Sepharose and Reactive Green 19-agarose Acetoanaerobium sticklandii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.103 234
-
 L-threonine, pH 9.0, 37°C Acetoanaerobium sticklandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.103 acetoin + NAD+ 1% of the activity compared to L-threonine Acetoanaerobium sticklandii butan-2,3-dione + NADH + H+
-
 ?
1.1.1.103 acetoin + NAD+ 1% of the activity compared to L-threonine Acetoanaerobium sticklandii DSM 519 butan-2,3-dione + NADH + H+
-
 ?
1.1.1.103 DL-3-hydroxynorvaline + NAD+ 20% of the activity compared to L-threonine Acetoanaerobium sticklandii 3-oxonorvaline + NADH + H+
-
 ?
1.1.1.103 DL-3-hydroxynorvaline + NAD+ 20% of the activity compared to L-threonine Acetoanaerobium sticklandii DSM 519 3-oxonorvaline + NADH + H+
-
 ?
1.1.1.103 L-threonine + NAD+
-
 Acetoanaerobium sticklandii (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?
1.1.1.103 L-threonine + NAD+
-
 Acetoanaerobium sticklandii DSM 519 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?
1.1.1.103 additional information no activity with L-theronine methyl ester, L-threonine amide, DL-threonine hydroxamate, L-homoserine, (R)-1-amino-2-propanol, L-malate, (R)-3-hydroxybutyrate, DL-threo-phenylserine, L-serine Acetoanaerobium sticklandii ?
-
 ?
1.1.1.103 additional information no activity with L-theronine methyl ester, L-threonine amide, DL-threonine hydroxamate, L-homoserine, (R)-1-amino-2-propanol, L-malate, (R)-3-hydroxybutyrate, DL-threo-phenylserine, L-serine Acetoanaerobium sticklandii DSM 519 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.103 homodimer 2 * 35000, SDS-PAGE, acitve in monomeric and in dimeric enzyme form Acetoanaerobium sticklandii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.103 CLOST_1621 locus name Acetoanaerobium sticklandii
1.1.1.103 threonine dehydrogenase
-
 Acetoanaerobium sticklandii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.103 5 8
-
 Acetoanaerobium sticklandii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.103 NAD+ NADP+, thio-NAD+, or 3-acetylpyridine adenine dinucleotide cannot substitute for NAD+ Acetoanaerobium sticklandii