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Literature summary extracted from

  • Tang, Z.; Salamanca-Pinzon, S.; Wu, Z.; Xiao, Y.; Guengerich, F.
    Human cytochrome P450 4F11: Heterologous expression in bacteria, purification, and characterization of catalytic function (2010), Arch. Biochem. Biophys., 494, 86-93.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.78 expressed in Escherichia coli BL21 cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.78 0.047
-
 palmitic acid at pH 7.4 and 37°C Homo sapiens
1.14.14.78 0.074
-
 3-hydroxypalmitate at pH 7.4 and 37°C Homo sapiens
1.14.14.78 0.08
-
 arachidonic acid at pH 7.4 and 37°C Homo sapiens
1.14.14.78 0.085
-
 oleic acid at pH 7.4 and 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.78 Fe2+ contains Fe2+ Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.78 50000
-
 x * 50000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.78 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.78 Ni2+-NTA agarose column chromatography, Biogel HTP hydroxylapatite column chromatography, and aminopropyl SPE column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.78 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.78 3-hydroxypalmitate + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens 3,16-dihydroxypalmitate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.78 arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens ?
-
 ?
1.14.14.78 oleic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens 18-hydroxyoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.78 palmitic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens 16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.78 ? x * 50000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.14.14.78 cytochrome P450 4F11
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.78 0.009
-
 arachidonic acid at pH 7.4 and 37°C Homo sapiens
1.14.14.78 0.02
-
 oleic acid at pH 7.4 and 37°C Homo sapiens
1.14.14.78 0.03
-
 palmitic acid at pH 7.4 and 37°C Homo sapiens
1.14.14.78 0.458
-
 3-hydroxypalmitate at pH 7.4 and 37°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.78 NADPH
-
 Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.14.78 0.12
-
 arachidonic acid at pH 7.4 and 37°C Homo sapiens
1.14.14.78 0.2
-
 oleic acid at pH 7.4 and 37°C Homo sapiens
1.14.14.78 0.63
-
 palmitic acid at pH 7.4 and 37°C Homo sapiens
1.14.14.78 6.17
-
 3-hydroxypalmitate at pH 7.4 and 37°C Homo sapiens