EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.1 | expression in Escherichia coli | uncultured organism |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.1 | Ba2+ | 2 mM, 72% residual activity | uncultured organism | |
3.2.1.1 | Bio-tex | liquid commercial detergent, 75% residual activity | uncultured organism | |
3.2.1.1 | Cu2+ | 2 mM, no residual activity | uncultured organism | |
3.2.1.1 | EDTA | 1 mM, almost complete loss of activity | uncultured organism | |
3.2.1.1 | Green Balance | solid commercial detergent, when used with tap water, 51% residual activity | uncultured organism | |
3.2.1.1 | Mg2+ | 2 mM, 58% residual activity | uncultured organism | |
3.2.1.1 | sodium dodecylsulfate | 0.1%, almost complete loss of activity | uncultured organism | |
3.2.1.1 | Zn2+ | 2 mM, no residual activity | uncultured organism |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.1 | additional information | - |
starch | Km value is 1.79 ml/ml at 5°C, pH 8.3 and ,328 mg/ml at 15°C, pH 8.3 | uncultured organism | |
3.2.1.1 | additional information | - |
amylopectin | Km value is 1.9 ml/ml at 5°C, pH 8.3 and 2.5 mg/ml at 15°C, pH 8.3 | uncultured organism |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.1 | Ca2+ | 2 mM, 127% of initial activity | uncultured organism |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.1 | uncultured organism | A0A060DAC6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.1 | recombinant enzyme | uncultured organism |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.1 | amylopectin + H2O | - |
uncultured organism | ? | - |
? | |
3.2.1.1 | amylose + H2O | - |
uncultured organism | ? | - |
? | |
3.2.1.1 | maltoheptaose + 3 H2O | - |
uncultured organism | 2 maltose + maltotriose | additional product: maltotetraose | ? | |
3.2.1.1 | maltopentaose + H2O | - |
uncultured organism | maltose + maltotriose | - |
? | |
3.2.1.1 | maltotetraose + H2O | - |
uncultured organism | 2 maltose | weak activity | ? | |
3.2.1.1 | additional information | no substrates: granular starch or glycogen, maltose, maltotriose | uncultured organism | ? | - |
? | |
3.2.1.1 | starch + H2O | - |
uncultured organism | maltose + ? | and larger maltooligosaccharides | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.1 | AmyI3C6 | - |
uncultured organism |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 10 | 15 | - |
uncultured organism |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 1 | - |
more than 70% of maximum activity | uncultured organism |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 34 | - |
or below, 60 min, no loss of activity | uncultured organism |
3.2.1.1 | 45 | - |
20 min, complete loss of activity | uncultured organism |
3.2.1.1 | 55 | - |
5 min, complete loss of activity | uncultured organism |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.1 | 0.03 | - |
starch | 5°C, pH 8.3 | uncultured organism | |
3.2.1.1 | 0.042 | - |
amylopectin | 5°C, pH 8.3 | uncultured organism | |
3.2.1.1 | 0.15 | - |
amylopectin | 15°C, pH 8.3 | uncultured organism | |
3.2.1.1 | 0.156 | - |
starch | 15°C, pH 8.3 | uncultured organism |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 8 | 9 | - |
uncultured organism |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | 6 | 10 | 20°C, stable for at least 14 h | uncultured organism |