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Literature summary extracted from

  • Tian, Q.; Song, P.; Jiang, L.; Li, S.; Huang, H.
    A novel cephalosporin deacetylating acetyl xylan esterase from Bacillus subtilis with high activity toward cephalosporin C and 7-aminocephalosporanic acid (2014), Appl. Microbiol. Biotechnol., 98, 2081-2089.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.41 gene Cah, subcloning in Escherichia coli strain JM109, expression in Escherichia coli strain BL21(DE3), the enzyme is secreted Bacillus subtilis
3.1.1.72 gene Cah, subcloning in Escherichia coli strain JM109, expression in Escherichia coli strain BL21(DE3), the enzyme is secreted Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.41 Ag+
-
 Bacillus subtilis
3.1.1.41 Ba2+ low inhibition at 1 mM Bacillus subtilis
3.1.1.41 Ca2+ low inhibition at 1 mM Bacillus subtilis
3.1.1.41 Cd2+ low inhibition at 1 mM Bacillus subtilis
3.1.1.41 Co2+ low inhibition at 1 mM Bacillus subtilis
3.1.1.41 Cu2+ strong inhibition at 1 mM Bacillus subtilis
3.1.1.41 diethyldicarbonate
-
 Bacillus subtilis
3.1.1.41 Fe2+ low inhibition at 1 mM Bacillus subtilis
3.1.1.41 Fe3+ strong inhibition at 1 mM Bacillus subtilis
3.1.1.41 Mg2+ low inhibition at 1 mM Bacillus subtilis
3.1.1.41 Mn2+ low inhibition at 1 mM Bacillus subtilis
3.1.1.41 additional information no inhibition by EDTA and K+ Bacillus subtilis
3.1.1.41 PMSF
-
 Bacillus subtilis
3.1.1.41 SDS low inhibition at 1 mM Bacillus subtilis
3.1.1.41 Zn2+ strong inhibition at 1 mM Bacillus subtilis
3.1.1.72 Ag+
-
 Bacillus subtilis
3.1.1.72 Ba2+ low inhibition at 1 mM Bacillus subtilis
3.1.1.72 Ca2+ low inhibition at 1 mM Bacillus subtilis
3.1.1.72 Cd2+ low inhibition at 1 mM Bacillus subtilis
3.1.1.72 Co2+ low inhibition at 1 mM Bacillus subtilis
3.1.1.72 Cu2+ strong inhibition at 1 mM Bacillus subtilis
3.1.1.72 diethyldicarbonate
-
 Bacillus subtilis
3.1.1.72 Fe2+ low inhibition at 1 mM Bacillus subtilis
3.1.1.72 Fe3+ strong inhibition at 1 mM Bacillus subtilis
3.1.1.72 Mg2+ low inhibition at 1 mM Bacillus subtilis
3.1.1.72 Mn2+ low inhibition at 1 mM Bacillus subtilis
3.1.1.72 additional information no inhibition by EDTA and K+ Bacillus subtilis
3.1.1.72 PMSF
-
 Bacillus subtilis
3.1.1.72 SDS low inhibition at 1 mM Bacillus subtilis
3.1.1.72 Zn2+ strong inhibition at 1 mM Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.41 36000
-
 6 * 36000, recombinant enzyme, SDS-PAGE Bacillus subtilis
3.1.1.41 223000
-
 recombinant enzyme, gel filtration Bacillus subtilis
3.1.1.72 36000
-
 6 * 36000, recombinant enzyme, SDS-PAGE Bacillus subtilis
3.1.1.72 223000
-
 recombinant enzyme, gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.41 additional information Bacillus subtilis the enzyme has a double specificity on both the acetylated oligosaccharide, cf. EC 3.1.1.72, and cephalosporin C and 7-aminocephalosporanic acid ?
-
 ?
3.1.1.41 additional information Bacillus subtilis CICC 20034 the enzyme has a double specificity on both the acetylated oligosaccharide, cf. EC 3.1.1.72, and cephalosporin C and 7-aminocephalosporanic acid ?
-
 ?
3.1.1.72 additional information Bacillus subtilis the enzyme has a double specificity on both the acetylated oligosaccharide and cephalosporin C and 7-aminocephalosporanic acid, cf. EC 3.1.1.41 ?
-
 ?
3.1.1.72 additional information Bacillus subtilis CICC 20034 the enzyme has a double specificity on both the acetylated oligosaccharide and cephalosporin C and 7-aminocephalosporanic acid, cf. EC 3.1.1.41 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.41 Bacillus subtilis M1JUH6 gene Cah
-
3.1.1.41 Bacillus subtilis CICC 20034 M1JUH6 gene Cah
-
3.1.1.72 Bacillus subtilis
-
 gene Cah
-
3.1.1.72 Bacillus subtilis CICC 20034
-
 gene Cah
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.41 recombinant enzyme 1.2fold from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, gel filtration, dialysis, and ultrafiltration Bacillus subtilis
3.1.1.72 recombinant enzyme 1.2fold from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, gel filtration, dialysis, and ultrafiltration Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.41 2.72
-
 purified recombinant enzyme, pH 7.0, 40°C, substrate 4-nitrophenyl butyrate Bacillus subtilis
3.1.1.41 741
-
 purified recombinant enzyme, pH 7.0, 40°C, substrate alpha-naphthyl acetate Bacillus subtilis
3.1.1.41 962
-
 purified recombinant enzyme, pH 7.0, 40°C, substrate beta-naphthyl acetate Bacillus subtilis
3.1.1.41 1086
-
 purified recombinant enzyme, pH 7.0, 40°C, substrate 4-methylumbelliferyl acetate Bacillus subtilis
3.1.1.41 1245
-
 purified recombinant enzyme, pH 7.0, 40°C, substrate glucose pentacetate Bacillus subtilis
3.1.1.41 2949
-
 purified recombinant enzyme, pH 7.0, 40°C, substrate 4-nitrophenyl acetate Bacillus subtilis
3.1.1.72 2.72
-
 purified recombinant enzyme, pH 7.0, 40°C, substrate 4-nitrophenyl butyrate Bacillus subtilis
3.1.1.72 741
-
 purified recombinant enzyme, pH 7.0, 40°C, substrate alpha-naphthyl acetate Bacillus subtilis
3.1.1.72 962
-
 purified recombinant enzyme, pH 7.0, 40°C, substrate beta-naphthyl acetate Bacillus subtilis
3.1.1.72 1086
-
 purified recombinant enzyme, pH 7.0, 40°C, substrate 4-methylumbelliferyl acetate Bacillus subtilis
3.1.1.72 1245
-
 purified recombinant enzyme, pH 7.0, 40°C, substrate glucose pentacetate Bacillus subtilis
3.1.1.72 2949
-
 purified recombinant enzyme, pH 7.0, 40°C, substrate 4-nitrophenyl acetate Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.41 2-naphthyl acetate + H2O
-
 Bacillus subtilis 2-naphthol + acetate
-
 ?
3.1.1.41 2-naphthyl acetate + H2O
-
 Bacillus subtilis CICC 20034 2-naphthol + acetate
-
 ?
3.1.1.41 3-naphthyl acetate + H2O
-
 Bacillus subtilis 3-naphthol + acetate
-
 ?
3.1.1.41 4-methylumbelliferyl acetate + H2O
-
 Bacillus subtilis 4-methylumbelliferol + acetate
-
 ?
3.1.1.41 4-nitrophenyl acetate + H2O
-
 Bacillus subtilis 4-nitrophenol + acetate
-
 ?
3.1.1.41 4-nitrophenyl acetate + H2O
-
 Bacillus subtilis CICC 20034 4-nitrophenol + acetate
-
 ?
3.1.1.41 4-nitrophenyl butyrate + H2O
-
 Bacillus subtilis 4-nitrophenol + butyrate
-
 ?
3.1.1.41 4-nitrophenyl butyrate + H2O
-
 Bacillus subtilis CICC 20034 4-nitrophenol + butyrate
-
 ?
3.1.1.41 glucose pentacetate + H2O
-
 Bacillus subtilis ?
-
 ?
3.1.1.41 additional information the enzyme has a double specificity on both the acetylated oligosaccharide, cf. EC 3.1.1.72, and cephalosporin C and 7-aminocephalosporanic acid Bacillus subtilis ?
-
 ?
3.1.1.41 additional information the enzyme hydrolyzes the ester linkages of the acetyl groups in both the xylose moieties of the acetylated xylan fragments Bacillus subtilis ?
-
 ?
3.1.1.41 additional information the enzyme has a double specificity on both the acetylated oligosaccharide, cf. EC 3.1.1.72, and cephalosporin C and 7-aminocephalosporanic acid Bacillus subtilis CICC 20034 ?
-
 ?
3.1.1.41 additional information the enzyme hydrolyzes the ester linkages of the acetyl groups in both the xylose moieties of the acetylated xylan fragments Bacillus subtilis CICC 20034 ?
-
 ?
3.1.1.72 2-naphthyl acetate + H2O
-
 Bacillus subtilis 2-naphthol + acetate
-
 ?
3.1.1.72 2-naphthyl acetate + H2O
-
 Bacillus subtilis CICC 20034 2-naphthol + acetate
-
 ?
3.1.1.72 3-naphthyl acetate + H2O
-
 Bacillus subtilis 3-naphthol + acetate
-
 ?
3.1.1.72 4-methylumbelliferyl acetate + H2O
-
 Bacillus subtilis 4-methylumbelliferol + acetate
-
 ?
3.1.1.72 4-nitrophenyl acetate + H2O
-
 Bacillus subtilis 4-nitrophenol + acetate
-
 ?
3.1.1.72 4-nitrophenyl acetate + H2O
-
 Bacillus subtilis CICC 20034 4-nitrophenol + acetate
-
 ?
3.1.1.72 4-nitrophenyl butyrate + H2O
-
 Bacillus subtilis 4-nitrophenol + butyrate
-
 ?
3.1.1.72 4-nitrophenyl butyrate + H2O
-
 Bacillus subtilis CICC 20034 4-nitrophenol + butyrate
-
 ?
3.1.1.72 glucose pentacetate + H2O
-
 Bacillus subtilis ?
-
 ?
3.1.1.72 additional information the enzyme has a double specificity on both the acetylated oligosaccharide and cephalosporin C and 7-aminocephalosporanic acid, cf. EC 3.1.1.41 Bacillus subtilis ?
-
 ?
3.1.1.72 additional information the enzyme hydrolyzes the ester linkages of the acetyl groups in both the xylose moieties of the acetylated xylan fragments Bacillus subtilis ?
-
 ?
3.1.1.72 additional information the enzyme has a double specificity on both the acetylated oligosaccharide and cephalosporin C and 7-aminocephalosporanic acid, cf. EC 3.1.1.41 Bacillus subtilis CICC 20034 ?
-
 ?
3.1.1.72 additional information the enzyme hydrolyzes the ester linkages of the acetyl groups in both the xylose moieties of the acetylated xylan fragments Bacillus subtilis CICC 20034 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.41 hexamer 6 * 36000, recombinant enzyme, SDS-PAGE Bacillus subtilis
3.1.1.72 hexamer 6 * 36000, recombinant enzyme, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.72 acetyl xylan esterase
-
 Bacillus subtilis
3.1.1.72 AXE
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.41 40
-
 assay at Bacillus subtilis
3.1.1.72 40
-
 assay at Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.41 30 75 activity range, profile overview Bacillus subtilis
3.1.1.72 30 75 activity range, profile overview Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.41 40
-
 purified recombinant enzyme, pH 7.0, stable up to, loss of 10% activity after 120 h Bacillus subtilis
3.1.1.41 50
-
 purified recombinant enzyme, pH 7.0, loss of 20% activity after 120 h Bacillus subtilis
3.1.1.41 50
-
 purified recombinant enzyme, pH 7.0, loss of 60% activity after 120 h Bacillus subtilis
3.1.1.41 60
-
 purified recombinant enzyme, pH 7.0, inactivation within 10 h Bacillus subtilis
3.1.1.72 40
-
 purified recombinant enzyme, pH 7.0, stable up to, loss of 10% activity after 120 h Bacillus subtilis
3.1.1.72 50
-
 purified recombinant enzyme, pH 7.0, loss of 20% activity after 120 h Bacillus subtilis
3.1.1.72 50
-
 purified recombinant enzyme, pH 7.0, loss of 60% activity after 120 h Bacillus subtilis
3.1.1.72 60
-
 purified recombinant enzyme, pH 7.0, inactivation within 10 h Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.41 7
-
 assay at Bacillus subtilis
3.1.1.72 7
-
 assay at Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.41 5 10 activity range, profile overview Bacillus subtilis
3.1.1.72 5 10 activity range, profile overview Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.41 4.5 12 purified recombinant enzyme, over 80% of maximal activity within this range Bacillus subtilis
3.1.1.72 4.5 12 purified recombinant enzyme, over 80% of maximal activity within this range Bacillus subtilis

General Information

EC Number General Information Comment Organism
3.1.1.41 evolution the enzyme belongs to carbohydrate esterase family 7 Bacillus subtilis
3.1.1.72 evolution the enzyme belongs to carbohydrate esterase family 7 Bacillus subtilis