Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dijkman, W.; Fraaije, M.
    Discovery and characterization of a 5-hydroxymethylfurfural oxidase from Methylovorus sp. strain MP688 (2014), Appl. Environ. Microbiol., 80, 1082-1090.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.47 expression in Escherichia coli Methylovorus sp. MP688

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.47 H467A catalysis of the mutant enzyme is severely reduced compared to that of the wild-type enzyme. With vanillyl alcohol as the substrate, the kcat value of the mutant enzyme is 4400 times lower than that of the wild-type enzyme Methylovorus sp. MP688

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.47 1.4
-
 5-(hydroxymethyl)furfural pH 8.0, 25°C Methylovorus sp. MP688

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.47 70000
-
 x * 70000, SDS-PAGE Methylovorus sp. MP688

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.47 5-(hydroxymethyl)furfural + 3 O2 + 2 H2O Methylovorus sp. MP688 the enzyme is involved in the degradation and detoxification of 5-(hydroxymethyl)furfural furan-2,5-dicarboxylate + 3 H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.47 Methylovorus sp. MP688 E4QP00
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.47
-
 Methylovorus sp. MP688

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.47 5-(dihydroxymethyl)furan-2-carbaldehyde + O2
-
 Methylovorus sp. MP688 5-formylfuran-2-carboxylate + H2O2 spontaneous formation of 5-(dihydroxymethyl)furan-2-carboxylate from 5-formylfuran-2-carboxylate ?
1.1.3.47 5-(dihydroxymethyl)furan-2-carboxylate + O2
-
 Methylovorus sp. MP688 furan-2,5-dicarboxylate + H2O2
-
 ?
1.1.3.47 5-(hydroxymethyl)furfural + 3 O2 + 2 H2O overall reaction Methylovorus sp. MP688 furan-2,5-dicarboxylate + 3 H2O2
-
 ?
1.1.3.47 5-(hydroxymethyl)furfural + 3 O2 + 2 H2O the enzyme is involved in the degradation and detoxification of 5-(hydroxymethyl)furfural Methylovorus sp. MP688 furan-2,5-dicarboxylate + 3 H2O2
-
 ?
1.1.3.47 5-(hydroxymethyl)furfural + O2
-
 Methylovorus sp. MP688 furan-2,5-dicarbaldehyde + H2O2 spontaneous formation of 5-(dihydroxymethyl)furan-2-carbaldehyde from furan-2,5-dicarbaldehyde ?
1.1.3.47 additional information the enzyme has a broad substrate range that overlaps with EC 1.1.3.7, aryl-alcohol oxidase Methylovorus sp. MP688 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.47 ? x * 70000, SDS-PAGE Methylovorus sp. MP688

Synonyms

EC Number Synonyms Comment Organism
1.1.3.47 5-hydroxymethylfurfural oxidase
-
 Methylovorus sp. MP688
1.1.3.47 HMF oxidase
-
 Methylovorus sp. MP688
1.1.3.47 HMFO
-
 Methylovorus sp. MP688

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.47 25
-
 assay at Methylovorus sp. MP688

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.47 9.9
-
 5-(hydroxymethyl)furfural pH 8.0, 25°C Methylovorus sp. MP688

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.47 8
-
 assay at Methylovorus sp. MP688

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.47 flavin the flavin cofactor is dissociable Methylovorus sp. MP688

General Information

EC Number General Information Comment Organism
1.1.3.47 metabolism the enzyme is involved in the degradation and detoxification of 5-(hydroxymethyl)furfural Methylovorus sp. MP688

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.47 7.1
-
 5-(hydroxymethyl)furfural pH 8.0, 25°C Methylovorus sp. MP688