Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Esteban-Torres, M.; Reveron, I.; Mancheno, J.M.; de Las Rivas, B.; Munoz, R.
    Characterization of a feruloyl esterase from Lactobacillus plantarum (2013), Appl. Environ. Microbiol., 79, 5130-5136.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.73 additional information the enzyme activity is not significantly affected by FeCl2, NiCl2, MnCl2, cysteine, CaCl2, MgCl2, DMSO, urea, and Triton X-100 Lactiplantibacillus plantarum
3.1.1.73 Tween 20 250% activation at 1 mM Lactiplantibacillus plantarum
3.1.1.73 Tween 80 250% activation at 1 mM Lactiplantibacillus plantarum
3.1.1.73 ZnCl2 155% activation at 1 mM Lactiplantibacillus plantarum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 gene lp_0796, cloning in Escherichia coli strain DH10B, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) from plasmid pURI3-TEV-0796 Lactiplantibacillus plantarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.73 2-mercaptoethanol
-
 Lactiplantibacillus plantarum
3.1.1.73 CuCl2
-
 Lactiplantibacillus plantarum
3.1.1.73 diethyldicarbonate
-
 Lactiplantibacillus plantarum
3.1.1.73 DTT strong inhibition Lactiplantibacillus plantarum
3.1.1.73 EDTA
-
 Lactiplantibacillus plantarum
3.1.1.73 ethyl acetate
-
 Lactiplantibacillus plantarum
3.1.1.73 HgCl2 strong inhibition Lactiplantibacillus plantarum
3.1.1.73 KCl
-
 Lactiplantibacillus plantarum
3.1.1.73 additional information the enzyme activity is not significantly affected by FeCl2, NiCl2, MnCl2, cysteine, CaCl2, MgCl2, DMSO, urea, and Triton X-100 Lactiplantibacillus plantarum
3.1.1.73 PMSF strong inhibition Lactiplantibacillus plantarum
3.1.1.73 pyridoxal 5'-phosphate
-
 Lactiplantibacillus plantarum
3.1.1.73 SDS strong inhibition Lactiplantibacillus plantarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.73 additional information the enzyme activity is not significantly affected by FeCl2, NiCl2, MnCl2, cysteine, CaCl2, MgCl2, DMSO, urea, and Triton X-100 Lactiplantibacillus plantarum

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Lactiplantibacillus plantarum F9UM18 gene lp_0796
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 benzyl cinnamate + H2O
-
 Lactiplantibacillus plantarum benzyl alcohol + cinnamate
-
 ?
3.1.1.73 butyl acetate + H2O
-
 Lactiplantibacillus plantarum butanol + acetate
-
 ?
3.1.1.73 ethyl acetate + H2O
-
 Lactiplantibacillus plantarum ethanol + acetate
-
 ?
3.1.1.73 ethyl bromoacetate + H2O
-
 Lactiplantibacillus plantarum ethanol + bromoacetate
-
 ?
3.1.1.73 ethyl butanoate + H2O
-
 Lactiplantibacillus plantarum ethanol + butyrate
-
 ?
3.1.1.73 ethyl decanoate + H2O
-
 Lactiplantibacillus plantarum ethanol + decanoate
-
 ?
3.1.1.73 ethyl glycine + H2O
-
 Lactiplantibacillus plantarum ethanol + glycine
-
 ?
3.1.1.73 ethyl hexanoate + H2O
-
 Lactiplantibacillus plantarum ethanol + hexanoate
-
 ?
3.1.1.73 ethyl octanoate + H2O
-
 Lactiplantibacillus plantarum ethanol + octanoate
-
 ?
3.1.1.73 ethyl oleate + H2O
-
 Lactiplantibacillus plantarum ethanol + oleate
-
 ?
3.1.1.73 isobutyl acetate + H2O
-
 Lactiplantibacillus plantarum isobutanol + acetate
-
 ?
3.1.1.73 isopropenyl acetate + H2O
-
 Lactiplantibacillus plantarum isopropenol + acetate
-
 ?
3.1.1.73 methyl (4-hydroxymethyl)benzoate + H2O
-
 Lactiplantibacillus plantarum methanol + (4-hydroxymethyl)benzoate
-
 ?
3.1.1.73 methyl 4-coumarate + H2O
-
 Lactiplantibacillus plantarum 4-coumarate + methanol
-
 ?
3.1.1.73 methyl benzoate + H2O
-
 Lactiplantibacillus plantarum methanol + benzoate
-
 ?
3.1.1.73 methyl bromoacetate + H2O
-
 Lactiplantibacillus plantarum methanol + bromoacetate
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Lactiplantibacillus plantarum caffeic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Lactiplantibacillus plantarum ferulate + methanol
-
 ?
3.1.1.73 methyl hydroxyacetate + H2O
-
 Lactiplantibacillus plantarum methanol + hydroxyacetate
-
 ?
3.1.1.73 methyl mandelate + H2O
-
 Lactiplantibacillus plantarum methanol + mandelate
-
 ?
3.1.1.73 methyl phenylacetate + H2O
-
 Lactiplantibacillus plantarum methanol + phenylacetate
-
 ?
3.1.1.73 methyl sinapinate + H2O
-
 Lactiplantibacillus plantarum sinapate + methanol
-
 ?
3.1.1.73 additional information the enzyme Lp_0796 is an esterase that, among other substrates, is able to efficiently hydrolyze the four model substrates for feruloyl esterases. No or poor activity with vinyl octanoate, ethyl trifluoroacetate, rosmarinic acid, chlorogenic acid, and trilaurin Lactiplantibacillus plantarum ?
-
 ?
3.1.1.73 phenyl acetate + H2O
-
 Lactiplantibacillus plantarum phenol + acetate
-
 ?
3.1.1.73 propyl acetate + H2O
-
 Lactiplantibacillus plantarum propanol + acetate
-
 ?
3.1.1.73 triacetin + H2O
-
 Lactiplantibacillus plantarum ?
-
 ?
3.1.1.73 tributyrin + H2O
-
 Lactiplantibacillus plantarum ?
-
 ?
3.1.1.73 vinyl acetate + H2O
-
 Lactiplantibacillus plantarum vinyl alcohol + acetate
-
 ?
3.1.1.73 vinyl benzoate + H2O
-
 Lactiplantibacillus plantarum vinyl alcohol + benzoate
-
 ?
3.1.1.73 vinyl butanoate + H2O
-
 Lactiplantibacillus plantarum vinyl alcohol + butanoate
-
 ?
3.1.1.73 vinyl decanoate + H2O
-
 Lactiplantibacillus plantarum vinyl alcohol + decanoate
-
 ?
3.1.1.73 vinyl hexadecanoate + H2O
-
 Lactiplantibacillus plantarum vinyl alcohol + hexadecanoate
-
 ?
3.1.1.73 vinyl hexanoate + H2O
-
 Lactiplantibacillus plantarum vinyl alcohol + hexanoate
-
 ?
3.1.1.73 vinyl propanoate + H2O
-
 Lactiplantibacillus plantarum vinyl alcohol + propanoate
-
 ?
3.1.1.73 vinyl tetradecanoate + H2O
-
 Lactiplantibacillus plantarum vinyl alcohol + tetradecanoate
-
 ?
3.1.1.73 vinyl trimethylacetate + H2O
-
 Lactiplantibacillus plantarum vinyl alcohol + trimethylacetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 Lp_0796
-
 Lactiplantibacillus plantarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 30 37
-
 Lactiplantibacillus plantarum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.73 22 45 marginal activity at 45°C Lactiplantibacillus plantarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 7
-
 assay at Lactiplantibacillus plantarum

Expression

EC Number Organism Comment Expression
3.1.1.73 Lactiplantibacillus plantarum the enzyme is induced by 3 mM methyl ferulate up