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Literature summary extracted from

  • Wang, L.; Zhang, R.; Ma, Z.; Wang, H.; Ng, T.
    A feruloyl esterase (FAE) characterized by relatively high thermostability from the edible mushroom Russula virescens (2014), Appl. Biochem. Biotechnol., 172, 993-1003.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.73 Al3+ 23% inhibition at 2.5 mM, 45% inhibition at 5 mM Russula virescens
3.1.1.73 Cd2+ 31% inhibition at 5 mM Russula virescens
3.1.1.73 Cu2+ 20% inhibition at 5 mM, 37% at 10 mM Russula virescens
3.1.1.73 Fe2+ complete inhibition at 5 mM Russula virescens
3.1.1.73 Hg2+ 60% inhibition at 1.25 mM, about 70% inhibition at 2.5-10 mM Russula virescens
3.1.1.73 K+ 10% inhibition at 5 mM, 20% at 10 mM Russula virescens
3.1.1.73 Mg2+ 8.5% inhibition at 5 mM, 22% at 10 mM Russula virescens
3.1.1.73 Pb2+ complete inhibition at 2.5 mM Russula virescens
3.1.1.73 Zn2+ 47% inhibition at 10 mM Russula virescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.19
-
 methyl ferulate pH 5.0, 50°C Russula virescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.73 additional information the enzyme activity is not significantly affected by Mn2+ at 2.5 mM Russula virescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 62000
-
 gel filtration Russula virescens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Russula virescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73 native enzyme 29.2fold from fruiting bodies by several steps of cation and anion exchange chromatography, and gel filtration Russula virescens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.73 fruit body fresh Russula virescens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 4-nitrophenyl butanoate + H2O
-
 Russula virescens 4-nitrophenol + butanoate
-
 ?
3.1.1.73 methyl 4-coumarate + H2O
-
 Russula virescens 4-coumarate + methanol
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Russula virescens caffeic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O preferred substrate Russula virescens ferulate + methanol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 monomer 1 * 62000 Russula virescens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FAE
-
 Russula virescens
3.1.1.73 ferulic acid esterase
-
 Russula virescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 50
-
-
 Russula virescens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.73 20 90 activity range, overview Russula virescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 40 60 purified native enzyme, 60 min, stable Russula virescens
3.1.1.73 70
-
 purified native enzyme, 60 min, over 70% residual activity Russula virescens
3.1.1.73 80
-
 purified native enzyme, 60 min, over 34% residual activity Russula virescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 5
-
-
 Russula virescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 4 6 75% of maximal activity at pH 4.0, maximal activity at pH 5.0, and 67% of maximal activity at pH 6.0, enzyme activity declined sharply above Russula virescens

General Information

EC Number General Information Comment Organism
3.1.1.73 evolution feruloyl esterases are a subclass of the carboxylic acid esterases, E.C. 3.1.1.1, that are able to hydrolyze the ester bond between hydroxycinnamic acids and sugars present in the plant cell walls Russula virescens