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Literature summary extracted from
- Purification and biochemical characterization of a novel thermo-stable carboxymethyl cellulase from Azorean isolate Bacillus mycoides S122C (2012), Appl. Biochem. Biotechnol., 168, 2191-2204.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.4 | 2-mercaptoethanol | 10 mM, 51% residual activity | Bacillus mycoides |  |
| 3.2.1.4 | CaCl2 | 5 mM, 52% residual activity | Bacillus mycoides | |
| 3.2.1.4 | CuSO4 | 5 mM, 38% residual activity | Bacillus mycoides | |
| 3.2.1.4 | dithiothreitol | 10 mM, 67% residual activity | Bacillus mycoides | |
| 3.2.1.4 | EDTA | 10 mM, 28% residual activity | Bacillus mycoides | |
| 3.2.1.4 | FeCl2 | 5 mM, 38% residual activity | Bacillus mycoides | |
| 3.2.1.4 | HgCl2 | 5 mM, 25% residual activity | Bacillus mycoides | |
| 3.2.1.4 | KCl | 5 mM, 74% residual activity | Bacillus mycoides | |
| 3.2.1.4 | MgCl2 | 5 mM, 52% residual activity | Bacillus mycoides | |
| 3.2.1.4 | NiCl2 | 5 mM, 48% residual activity | Bacillus mycoides | |
| 3.2.1.4 | sodium dodecylsulfate | 1%, 73% residual activity | Bacillus mycoides | |
| 3.2.1.4 | Tween-20 | 1%, 79% residual activity | Bacillus mycoides | |
| 3.2.1.4 | Urea | 10 mM, 45% residual activity | Bacillus mycoides | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.4 | extracellular | - |
Bacillus mycoides | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 62000 | - |
x * 62000, SDS-PAGE | Bacillus mycoides |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.4 | Bacillus mycoides | - |
- |
- |
| 3.2.1.4 | Bacillus mycoides S122C | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.4 | - |
Bacillus mycoides |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.4 | culture medium | - |
Bacillus mycoides | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.4 | beta-glucan + H2O | - |
Bacillus mycoides | ? | - |
? | |
| 3.2.1.4 | beta-glucan + H2O | - |
Bacillus mycoides S122C | ? | - |
? | |
| 3.2.1.4 | carboxymethylcellulose + H2O | best substrate | Bacillus mycoides | ? | - |
? | |
| 3.2.1.4 | carboxymethylcellulose + H2O | best substrate | Bacillus mycoides S122C | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | ? | x * 62000, SDS-PAGE | Bacillus mycoides |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.4 | CMcellulase | - |
Bacillus mycoides |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 50 | - |
- |
Bacillus mycoides |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 40 | 60 | - |
Bacillus mycoides |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 7 | - |
- |
Bacillus mycoides |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.4 | 5 | 10 | more than 60% of maximum activity | Bacillus mycoides |
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