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Literature summary extracted from

  • Sakaguchi, M.; Matsushima, Y.; Nankumo, T.; Seino, J.; Miyakawa, S.; Honda, S.; Sugahara, Y.; Oyama, F.; Kawakita, M.
    Glucoamylase of Caulobacter crescentus CB15: Cloning and expression in Escherichia coli and functional identification (2014), AMB Express, 4, 5.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.3 expression in Escherichia coli, as fusion protein with Staphylococcus protein A Caulobacter vibrioides

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.3 additional information CauloGA gene product that is expressed in Escherichia coli is prone to forming inclusion bodies. Most of the gene product is expressed in a soluble and active form when it was expressed as a fusion protein with Staphylococcus Protein A Caulobacter vibrioides

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.3 maltoheptaose
-
 Caulobacter vibrioides
3.2.1.3 maltohexaose
-
 Caulobacter vibrioides
3.2.1.3 maltopentaose
-
 Caulobacter vibrioides
3.2.1.3 maltose
-
 Caulobacter vibrioides
3.2.1.3 maltotetraose
-
 Caulobacter vibrioides
3.2.1.3 maltotriose pronounced inhibition above 2 mM Caulobacter vibrioides
3.2.1.3 additional information enzyme activity is significantly inhibited when the substrate concentration exceeds approximately 10fold the Km value Caulobacter vibrioides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 0.03
-
 maltopentaose pH 5.0, 40°C Caulobacter vibrioides
3.2.1.3 0.04
-
 maltoheptaose pH 5.0, 40°C Caulobacter vibrioides
3.2.1.3 0.05
-
 maltohexaose pH 5.0, 40°C Caulobacter vibrioides
3.2.1.3 0.14
-
 maltotetraose pH 5.0, 40°C Caulobacter vibrioides
3.2.1.3 0.16
-
 maltotriose pH 5.0, 40°C Caulobacter vibrioides
3.2.1.3 0.87
-
 maltose pH 5.0, 40°C Caulobacter vibrioides

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Caulobacter vibrioides Q9A613
-
-
3.2.1.3 Caulobacter vibrioides CB15 Q9A613
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 maltoheptaose + 6 H2O
-
 Caulobacter vibrioides 7 beta-D-glucose
-
 ?
3.2.1.3 maltoheptaose + 6 H2O
-
 Caulobacter vibrioides CB15 7 beta-D-glucose
-
 ?
3.2.1.3 maltohexaose + 5 H2O
-
 Caulobacter vibrioides 6 beta-D-glucose
-
 ?
3.2.1.3 maltohexaose + 5 H2O
-
 Caulobacter vibrioides CB15 6 beta-D-glucose
-
 ?
3.2.1.3 maltopentaose + 4 H2O
-
 Caulobacter vibrioides 5 beta-D-glucose
-
 ?
3.2.1.3 maltopentaose + 4 H2O
-
 Caulobacter vibrioides CB15 5 beta-D-glucose
-
 ?
3.2.1.3 maltose + H2O
-
 Caulobacter vibrioides 2 beta-D-glucose
-
 ?
3.2.1.3 maltotetraose + 3 H2O
-
 Caulobacter vibrioides 4 beta-D-glucose
-
 ?
3.2.1.3 maltotriose + 2 H2O
-
 Caulobacter vibrioides 3 beta-D-glucose
-
 ?
3.2.1.3 maltotriose + 2 H2O
-
 Caulobacter vibrioides CB15 3 beta-D-glucose
-
 ?
3.2.1.3 starch + H2O
-
 Caulobacter vibrioides beta-D-glucose + ?
-
 ?
3.2.1.3 starch + H2O
-
 Caulobacter vibrioides CB15 beta-D-glucose + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 40
-
-
 Caulobacter vibrioides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 42.9
-
 melting temperature Caulobacter vibrioides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.3 38.2
-
 maltose pH 5.0, 40°C Caulobacter vibrioides
3.2.1.3 114
-
 maltoheptaose pH 5.0, 40°C Caulobacter vibrioides
3.2.1.3 122
-
 maltopentaose pH 5.0, 40°C Caulobacter vibrioides
3.2.1.3 125
-
 maltohexaose pH 5.0, 40°C Caulobacter vibrioides
3.2.1.3 150
-
 maltotriose pH 5.0, 40°C Caulobacter vibrioides
3.2.1.3 155
-
 maltotetraose pH 5.0, 40°C Caulobacter vibrioides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 5
-
-
 Caulobacter vibrioides

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.3 0.92
-
 maltopentaose pH 5.0, 40°C Caulobacter vibrioides
3.2.1.3 1.05
-
 maltohexaose pH 5.0, 40°C Caulobacter vibrioides
3.2.1.3 1.1
-
 maltotetraose pH 5.0, 40°C Caulobacter vibrioides
3.2.1.3 1.25
-
 maltoheptaose pH 5.0, 40°C Caulobacter vibrioides
3.2.1.3 4.49
-
 maltotriose pH 5.0, 40°C Caulobacter vibrioides
3.2.1.3 38.2
-
 maltose pH 5.0, 40°C Caulobacter vibrioides

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.3 43.9
-
 maltose pH 5.0, 40°C Caulobacter vibrioides
3.2.1.3 938
-
 maltotriose pH 5.0, 40°C Caulobacter vibrioides
3.2.1.3 1110
-
 maltotetraose pH 5.0, 40°C Caulobacter vibrioides
3.2.1.3 2500
-
 maltohexaose pH 5.0, 40°C Caulobacter vibrioides
3.2.1.3 2850
-
 maltoheptaose pH 5.0, 40°C Caulobacter vibrioides
3.2.1.3 4070
-
 maltopentaose pH 5.0, 40°C Caulobacter vibrioides