Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Vandavasi, V.; Taylor-Creel, K.; McFeeters, R.L.; Coates, L.; McFeeters, H.
    Recombinant production, crystallization and X-ray crystallographic structure determination of peptidyl-tRNA hydrolase from Salmonella typhimurium (2014), Acta Crystallogr. Sect. F, 70, 872-877.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.29 expressed in Escherichia coli BL21 pLysS cells Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.29 hanging drop vapor diffusion method, using 0.03 M citric acid, 0.05 M Bis-Tris propane, 1% (v/v) glycerol, 3% (w/v) sucrose, 25% (w/v) PEG 6000 pH 7.6 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.29 25000
-
 x * 25000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.29 Salmonella enterica subsp. enterica serovar Typhimurium A0A0F6B281
-
-
3.1.1.29 Salmonella enterica subsp. enterica serovar Typhimurium 14028s A0A0F6B281
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.29 Ni2+-chelating affinity column chromatography and Superose 12 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
3.1.1.29 22°C, 20 mM Bis-Tris pH 6.6, 50 mM NaCl, 2 mM dithiothreitol, several weeks, without loss of activity or precipitation Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.29 peptidyl-tRNA + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
3.1.1.29 peptidyl-tRNA + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium 14028s ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.29 ? x * 25000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

EC Number Synonyms Comment Organism
3.1.1.29 peptidyl-tRNA hydrolase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
3.1.1.29 Pth1
-
 Salmonella enterica subsp. enterica serovar Typhimurium