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Literature summary extracted from

  • Zhang, X.;, Shang, G.; Gu, L.; Shen Y.
    Crystallization and preliminary X-ray diffraction analysis of the diterpene cyclooctatin synthase (CYC) from Streptomyces sp. LZ35 (2014), Acta Crystallogr. Sect. F, 70, 366-369.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.146 expressed in Escherichia coli BL21(DE3) cells Streptomyces sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.3.146 sitting drop vapor diffusion method, using 0.8 M NaH2PO4/K2HPO4 pH 7.8 Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.146 geranylgeranyl diphosphate + H2O Streptomyces sp.
-
 cyclooctat-9-en-7-ol + diphosphate
-
 ?
4.2.3.146 geranylgeranyl diphosphate + H2O Streptomyces sp. LZ35
-
 cyclooctat-9-en-7-ol + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.146 Streptomyces sp.
-
-
-
4.2.3.146 Streptomyces sp. LZ35
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.146 Ni-chelating Sepharose affinity column chromatography and Superdex 200gel filtration Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.146 geranylgeranyl diphosphate + H2O
-
 Streptomyces sp. cyclooctat-9-en-7-ol + diphosphate
-
 ?
4.2.3.146 geranylgeranyl diphosphate + H2O
-
 Streptomyces sp. LZ35 cyclooctat-9-en-7-ol + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.146 CYC
-
 Streptomyces sp.
4.2.3.146 diterpene cyclooctatin synthase
-
 Streptomyces sp.