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Literature summary extracted from

  • Matsumoto, A.; Shimizu, Y.; Takemoto, C.; Ueda, T.; Uchiumi, T.; Ito, K.
    Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Thermus thermophilus HB8 (2013), Acta Crystallogr. Sect. F, 69, 332-335.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.29 expressed in Escherichia coli BL21(DE3) cells Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.29 sitting drop vapor diffusion method, using 100 mM phosphate-citrate buffer pH 4.2, and 50% (w/v) 2-methyl-2,4-pentanediol Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.29 N-substituted aminoacyl-tRNA + H2O Thermus thermophilus
-
 N-substituted amino acid + tRNA
-
 ?
3.1.1.29 N-substituted aminoacyl-tRNA + H2O Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 N-substituted amino acid + tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.29 Thermus thermophilus
-
-
-
3.1.1.29 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.29 HisTrap column chromatography, Ni-NTA agarose column chromatography, and Q Sepharose column chromatography Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.29 N-substituted aminoacyl-tRNA + H2O
-
 Thermus thermophilus N-substituted amino acid + tRNA
-
 ?
3.1.1.29 N-substituted aminoacyl-tRNA + H2O
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 N-substituted amino acid + tRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.29 peptidyl-tRNA hydrolase
-
 Thermus thermophilus
3.1.1.29 PTH
-
 Thermus thermophilus