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Literature summary extracted from
- Cloning, expression, purification, crystallization and preliminary X-ray studies of argininosuccinate lyase (Rv1659) from Mycobacterium tuberculosis (2013), Acta Crystallogr. Sect. F, 69, 1422-1424.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.3.2.1 | gene argH, expression of Rv1659 as His-tagged protein, with an extra methionine at the N-terminus and an extended sequence LEHHHHHH at the C-terminus, in Escherichia coli strain BL21 (DE3) | Mycobacterium tuberculosis |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 4.3.2.1 | purified recombinant His-tagged enzyme, microbatch-under-oil method, mixing of 0.002 ml of 9.5 mg/ml protein in 25 mM phosphate buffer, pH 7.4, with 0.002 ml of precipitant solution containing 100 mM Bis-Tris, pH 5.5, 25% w/v polyethylene glycol 3350, X-ray diffraction structure determination and analysis at 2.40 A resolution, molecular replacement method | Mycobacterium tuberculosis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.3.2.1 | 50900 | - |
4 * 50900, recombinant His-tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.3.2.1 | Mycobacterium tuberculosis | P9WPY7 | gene Rv1659 | - |
| 4.3.2.1 | Mycobacterium tuberculosis H37Rv | P9WPY7 | gene Rv1659 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.3.2.1 | recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and dialysis to homogeneity | Mycobacterium tuberculosis |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.3.2.1 | homotetramer | 4 * 50900, recombinant His-tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.3.2.1 | ArgH | - |
Mycobacterium tuberculosis |
| 4.3.2.1 | Rv1659 | - |
Mycobacterium tuberculosis |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.3.2.1 | metabolism | last enzyme in the arginine biosynthesis pathway | Mycobacterium tuberculosis |
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