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Literature summary extracted from

  • Siddiqui, T.; Paxman, J.J.; Dogovski, C.; Panjikar, S.; Perugini, M.A.
    Cloning to crystallization of dihydrodipicolinate synthase from the intracellular pathogen Legionella pneumophila (2013), Acta Crystallogr. Sect. F, 69, 1177-1181.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.3.7 DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21(DE3) Legionella pneumophila

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.3.7 purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.002 ml of 11 mg/ml protein in 20 mM Tris, pH 7.5, with 0.002 ml of reservoir solution containing 0.2 M magnesium chloride, 10% w/v PEG 8000, 0.1 M Tris chloride, pH 10, at 8°C, X-ray diffraction structure determination and analysis at 1.65-1.74 A resolution, molecular replacement Legionella pneumophila

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.3.7 (S)-aspartate-4-semialdehyde + pyruvate Legionella pneumophila
-
 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.7 Legionella pneumophila
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.7 recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography, followed by dialysis Legionella pneumophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.7 (S)-aspartate-4-semialdehyde + pyruvate
-
 Legionella pneumophila (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.3.7 DHDPS
-
 Legionella pneumophila
4.3.3.7 dihydrodipicolinate synthase
-
 Legionella pneumophila

General Information

EC Number General Information Comment Organism
4.3.3.7 metabolism the enzyme catalyses the first committed step in the lysine biosynthesis pathway, the condensation of pyruvate and (S)-aspartate semialdehyde to form (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid Legionella pneumophila