EC Number | Cloned (Comment) | Organism |
---|---|---|
4.3.3.7 | gene dapA, sequence comparison with Escherichia coli enzyme, expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Agrobacterium tumefaciens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.3.3.7 | purified recombinant His-tagged enzyme in the unliganded form and in forms with bound substrate and with bound substrate plus allosteric inhibitor lysine, hanging drop vapour diffusion method, mixing of 0.002 ml of 10 mg/ml protein in 20 mM Tris, pH 8.0, with 0.002 ml of reservoir solution containing 0.1 M Tris, pH 8.5, and 2 M ammonium sulfate, for ligand-bound enzyme from 0.17 M lithium sulfate monohydrate, 0.085 M Tris pH 8.5, 25.5%(w/v) PEG 4000, 15%(v/v) glycerol with addition of 20 mM pyruvate or 20 mM pyruvate and 20 mM lysine, equilibration against 1 ml reservoir solution, 20°C, overnight, X-ray diffraction structure determination and analysis at 1.40-1.55 A resolution | Agrobacterium tumefaciens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.3.3.7 | D-Lysine | allosteric inhibitor | Agrobacterium tumefaciens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.3.7 | (S)-aspartate-4-semialdehyde + pyruvate | Agrobacterium tumefaciens | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O | - |
? | |
4.3.3.7 | (S)-aspartate-4-semialdehyde + pyruvate | Agrobacterium tumefaciens C58 / ATCC 33970 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.3.7 | Agrobacterium tumefaciens | Q8UGL3 | gene dapA | - |
4.3.3.7 | Agrobacterium tumefaciens C58 / ATCC 33970 | Q8UGL3 | gene dapA | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.3.3.7 | recombinant His-tagged enzyme 8fold from Escherichia coli strain BL21 (DE3) by immobilized metal affinity chromatography and gel filtration | Agrobacterium tumefaciens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.3.3.7 | pyruvate + L-aspartate-4-semialdehyde = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O | ping-pong mechanism, in which pyruvate binds as a Schiff base to an active-site lysine residue (Lys162 in Agrobacterium tumefaciens) | Agrobacterium tumefaciens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.3.3.7 | 174 | - |
purified recombinant His-tagged enzyme, coupled assay, pH and temperature not specified in the publication | Agrobacterium tumefaciens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.3.7 | (S)-aspartate-4-semialdehyde + pyruvate | - |
Agrobacterium tumefaciens | (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O | - |
? | |
4.3.3.7 | (S)-aspartate-4-semialdehyde + pyruvate | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.3.3.7 | DHDPS | - |
Agrobacterium tumefaciens |
4.3.3.7 | dihydrodipicolinate synthase | - |
Agrobacterium tumefaciens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.3.3.7 | metabolism | the enzyme catalyses the first committed step in the lysine biosynthesis pathway, the condensation of pyruvate and (S)-aspartate semialdehyde to form (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid | Agrobacterium tumefaciens |