Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Atkinson, S.C.; Dogovski, C.; Newman, J.; Dobson, R.C.; Perugini, M.A.
    Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from the grapevine Vitis vinifera (2011), Acta Crystallogr. Sect. F, 67, 1537-1541.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.3.7 expression of N-terminally His6-tagged enzyme with a thrombin cleavage site in Escherichia coli strain BL21 (DE3) Vitis vinifera

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.3.7 purifed recombinant detagged enzyme in complex with pyruvate, hanging drop vapour diffusion method, mixing of 0.002 ml of 10 mg/ml protein in 20 mM Tris, 150 mM NaCl, pH 8.0, and 20 mM pyruvate, with 0.002 ml of reservoir solution containing 0.1 M Bis-Tris propane, pH 8.2, 0.2 M sodium bromide, and 20% w/v PEG 3350, equilibration against 1 ml of reservoir solution, 20°C, method ooptimization, X-ray diffraction structure determination and analysis at 2.2 A resolution Vitis vinifera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.3.7 (S)-aspartate-4-semialdehyde + pyruvate Vitis vinifera
-
 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.7 Vitis vinifera
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.7 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography, tag cleavage by thrombin, and gel filtration Vitis vinifera

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.7 (S)-aspartate-4-semialdehyde + pyruvate
-
 Vitis vinifera (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.3.7 DHDPS
-
 Vitis vinifera
4.3.3.7 dihydrodipicolinate synthase
-
 Vitis vinifera