BRENDA - Enzyme Database show

Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from the grapevine Vitis vinifera

Atkinson, S.C.; Dogovski, C.; Newman, J.; Dobson, R.C.; Perugini, M.A.; Acta Crystallogr. Sect. F 67, 1537-1541 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.3.3.7
expression of N-terminally His6-tagged enzyme with a thrombin cleavage site in Escherichia coli strain BL21 (DE3)
Vitis vinifera
Crystallization (Commentary)
EC Number
Crystallization
Organism
4.3.3.7
purifed recombinant detagged enzyme in complex with pyruvate, hanging drop vapour diffusion method, mixing of 0.002 ml of 10 mg/ml protein in 20 mM Tris, 150 mM NaCl, pH 8.0, and 20 mM pyruvate, with 0.002 ml of reservoir solution containing 0.1 M Bis-Tris propane, pH 8.2, 0.2 M sodium bromide, and 20% w/v PEG 3350, equilibration against 1 ml of reservoir solution, 20°C, method ooptimization, X-ray diffraction structure determination and analysis at 2.2 A resolution
Vitis vinifera
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.3.7
(S)-aspartate-4-semialdehyde + pyruvate
Vitis vinifera
-
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.3.7
Vitis vinifera
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.3.7
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography, tag cleavage by thrombin, and gel filtration
Vitis vinifera
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
(S)-aspartate-4-semialdehyde + pyruvate
-
728889
Vitis vinifera
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
expression of N-terminally His6-tagged enzyme with a thrombin cleavage site in Escherichia coli strain BL21 (DE3)
Vitis vinifera
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.3.3.7
purifed recombinant detagged enzyme in complex with pyruvate, hanging drop vapour diffusion method, mixing of 0.002 ml of 10 mg/ml protein in 20 mM Tris, 150 mM NaCl, pH 8.0, and 20 mM pyruvate, with 0.002 ml of reservoir solution containing 0.1 M Bis-Tris propane, pH 8.2, 0.2 M sodium bromide, and 20% w/v PEG 3350, equilibration against 1 ml of reservoir solution, 20°C, method ooptimization, X-ray diffraction structure determination and analysis at 2.2 A resolution
Vitis vinifera
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.3.7
(S)-aspartate-4-semialdehyde + pyruvate
Vitis vinifera
-
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography, tag cleavage by thrombin, and gel filtration
Vitis vinifera
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
(S)-aspartate-4-semialdehyde + pyruvate
-
728889
Vitis vinifera
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
-
-
?