BRENDA - Enzyme Database show

Crystallization and preliminary X-ray diffraction analysis of dihydrodipicolinate synthase 2 from Arabidopsis thaliana

Griffin, M.D.; Billakanti, J.M.; Gerrard, J.A.; Dobson, R.C.; Pearce, F.G.; Acta Crystallogr. Sect. F 67, 1386-1390 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.3.3.7
orf AT2G45440, recombinant His-tagged enzyme expression in Escherichia coli strain BL21 (DE3) Star
Arabidopsis thaliana
Crystallization (Commentary)
EC Number
Crystallization
Organism
4.3.3.7
purified recombinant His-tagged enzyme, sitting drop vapour diffusion method, 0.0015 ml of 14.5 mg/ml protein in 20 mM Tris-HCl, pH 8.0 is mixed with 0.0015 ml of reservoir solution containing 20% w/v PEG 6000, 200 mM sodium chloride, 100 mM Tris-HCl, pH 8.0, including 0.02% w/v sodium azide, 20C, method screening, 5 days to 8 weeks, X-ray diffraction structure determination and analysis at 2.5 A resolution
Arabidopsis thaliana
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.3.3.7
chloroplast
-
Arabidopsis thaliana
9507
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
34680
-
x * 34680, mass spectrometry
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.3.7
(S)-aspartate-4-semialdehyde + pyruvate
Arabidopsis thaliana
-
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.3.7
Arabidopsis thaliana
Q9FVC8
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.3.7
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) Star by nickel affinity chromatography, cleavage of the His-tag by TEV protease
Arabidopsis thaliana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
(S)-aspartate-4-semialdehyde + pyruvate
-
728886
Arabidopsis thaliana
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.3.3.7
?
x * 34680, mass spectrometry
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
orf AT2G45440, recombinant His-tagged enzyme expression in Escherichia coli strain BL21 (DE3) Star
Arabidopsis thaliana
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.3.3.7
purified recombinant His-tagged enzyme, sitting drop vapour diffusion method, 0.0015 ml of 14.5 mg/ml protein in 20 mM Tris-HCl, pH 8.0 is mixed with 0.0015 ml of reservoir solution containing 20% w/v PEG 6000, 200 mM sodium chloride, 100 mM Tris-HCl, pH 8.0, including 0.02% w/v sodium azide, 20C, method screening, 5 days to 8 weeks, X-ray diffraction structure determination and analysis at 2.5 A resolution
Arabidopsis thaliana
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.3.3.7
chloroplast
-
Arabidopsis thaliana
9507
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.3.3.7
34680
-
x * 34680, mass spectrometry
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.3.7
(S)-aspartate-4-semialdehyde + pyruvate
Arabidopsis thaliana
-
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) Star by nickel affinity chromatography, cleavage of the His-tag by TEV protease
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
(S)-aspartate-4-semialdehyde + pyruvate
-
728886
Arabidopsis thaliana
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.3.3.7
?
x * 34680, mass spectrometry
Arabidopsis thaliana
General Information
EC Number
General Information
Commentary
Organism
4.3.3.7
metabolism
the enzyme catalyzes the branch-point reaction in the biosynthetic pathway leading to meso-diaminopimelate and (S)-lysine in plants and bacteria: condensation of (S)-aspartate-4-semialdehyde and pyruvate to form (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid. There are four variants of the meso-diaminopimelate/(S)-lysine pathway, they all share the same enzymatic steps for the synthesis of tetrahydrodipicolinate from aspartate, which includes the reaction catalyzed by the enzyme, overview
Arabidopsis thaliana
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.3.3.7
metabolism
the enzyme catalyzes the branch-point reaction in the biosynthetic pathway leading to meso-diaminopimelate and (S)-lysine in plants and bacteria: condensation of (S)-aspartate-4-semialdehyde and pyruvate to form (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid. There are four variants of the meso-diaminopimelate/(S)-lysine pathway, they all share the same enzymatic steps for the synthesis of tetrahydrodipicolinate from aspartate, which includes the reaction catalyzed by the enzyme, overview
Arabidopsis thaliana